The Prevention of Diabetic Cardiomyopathy by Non-Mitogenic Acidic Fibroblast Growth Factor Is Probably Mediated by the Suppression of Oxidative Stress and Damage

doi:10.1371/journal.pone.0082287

科研成果详情

题名	The Prevention of Diabetic Cardiomyopathy by Non-Mitogenic Acidic Fibroblast Growth Factor Is Probably Mediated by the Suppression of Oxidative Stress and Damage
作者	Zhang, Chi 1,2; Zhang, Linbo 3,4; Chen, Shali 3; Feng, Biao 3; Lu, Xuemian2; Bai, Yang 5; Liang, Guang1,6; Tan, Yi1,7,8; Shao, Minglong1,2; Skibba, Melissa 7; Jin, Litai1,6; Li, Xiaokun1,5; Chakrabarti, Subrata 3; Cai, Lu1,7,8
发表日期	2013-12-09
发表期刊	PLOS ONE 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	NF-KAPPA-B ; CARDIAC MYOCYTES ; MYOCARDIAL FIBROSIS ; FACTOR FAMILY ; DNA-DAMAGE ; APOPTOSIS ; HEART ; HYPERTROPHY ; DYSFUNCTION ; RATS
摘要	Background: Emerging evidence showed the beneficial effect of acidic fibroblast growth factor (aFGF) on heart diseases. The present study investigated whether non-mitogenic aFGF (nm-aFGF) can prevent diabetic cardiomyopathy and the underlying mechanisms, if any. Methodology/Principal Findings: Type 1 diabetes was induced in mice by multiple intraperitoneal injections of low-dose streptozotocin. Hyperglycemic and age-matched control mice were treated with or without nm-aFGF at 10 mu g/kg daily for 1 and 6 months. Blood pressure and cardiac function were assessed. Cardiac H9c2 cell, human microvascular endothelial cells, and rat cardiomyocytes were exposed to high glucose (25 mM) for mimicking an in vitro diabetic condition for mechanistic studies. Oxidative stress, DNA damage, cardiac hypertrophy and fibrosis were assessed by real-time qPCR, immunofluorescent staining, Western blotting, and pathological examination. Nm-aFGF significantly prevented diabetes-induced hypertension and cardiac dysfunction at 6 months. Mechanistic studies demonstrated that nm-aFGF showed the similar preventive effect as the native aFGF on high glucose-induced oxidative stress (increase generation of reactive oxygen species) and damage (cellular DNA oxidation), cell hypertrophy, and fibrotic response (increased mRNA expression of fibronectin) in three kinds of cells. These in vitro findings were recaptured by examining the heart of the diabetic mice with and without nm-aFGF. Conclusions: These results suggest that nm-aFGF can prevent diabetic cardiomyopathy, probably through attenuation of cardiac oxidative stress, hypertrophy, and fibrosis.
资助项目	National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81000294]; Wenzhou Medical University [QTJ13005]; China-Canada Joint Health Research Initiative [81061120517]; Starting-Up Fund for Chinese-American Research Institute for Diabetic Complications from Wenzhou Medical University
出版者	PUBLIC LIBRARY SCIENCE
出版地	SAN FRANCISCO
ISSN	1932-6203
卷号	8 期号:12 页码:e82287.
DOI	10.1371/journal.pone.0082287
页数	10
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000328705200073
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	24349248
PMC记录号	PMC3857250
SCOPUSEID	2-s2.0-84891937623
通讯作者地址	[Chakrabarti, Subrata]Department of Pathology,Western University,Canada
Scopus学科分类	Multidisciplinary
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/9957
专题	温州医科大学药学院（分析测试中心）其他_附属第三医院（瑞安市人民医院）
通讯作者	Chakrabarti, Subrata
作者单位	1.Chinese-American Research Institute for Diabetic Complications,Wenzhou Medical University,China; 2.Ruian Center of the Chinese-American Research Institute for Diabetic Complications,Third Affiliated Hospital,Wenzhou Medical University,China; 3.Department of Pathology,Western University,Canada; 4.Department of Pharmaceutical Engineering,Jilin Agriculture University,China; 5.Department of Cardiac Surgery,First Hospital,Jilin University,China; 6.School of Pharmacy,Wenzhou Medical University,China; 7.Department of Pharmacology and Toxicology,University of Louisville,United States; 8.Kosair Children's Hospital Research Institute,Department of Pediatrics,University of Louisville,United States
第一作者单位	温州医科大学; 其他_附属第三医院（瑞安市人民医院）
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Zhang, Chi,Zhang, Linbo,Chen, Shali,et al. The Prevention of Diabetic Cardiomyopathy by Non-Mitogenic Acidic Fibroblast Growth Factor Is Probably Mediated by the Suppression of Oxidative Stress and Damage[J]. PLOS ONE,2013,8(12):e82287..
APA	Zhang, Chi., Zhang, Linbo., Chen, Shali., Feng, Biao., Lu, Xuemian., ... & Cai, Lu. (2013). The Prevention of Diabetic Cardiomyopathy by Non-Mitogenic Acidic Fibroblast Growth Factor Is Probably Mediated by the Suppression of Oxidative Stress and Damage. PLOS ONE, 8(12), e82287..
MLA	Zhang, Chi,et al."The Prevention of Diabetic Cardiomyopathy by Non-Mitogenic Acidic Fibroblast Growth Factor Is Probably Mediated by the Suppression of Oxidative Stress and Damage".PLOS ONE 8.12(2013):e82287..