Inhibition of forkhead box O1 protects pancreatic β-cells against dexamethasone-induced dysfunction

doi:10.1210/en.2009-0343

科研成果详情

题名	Inhibition of forkhead box O1 protects pancreatic β-cells against dexamethasone-induced dysfunction
作者	Zhang, Xiongfei1,4; Yong, Wei 1; Lv, Jinghuan 1; Zhu, Yunxia 1; Zhang, Jingjing 3; Chen, Fang 1; Zhang, Rihua 1; Yang, Tao 2; Sun, Yujie 1,2; Han, Xiao 1,2
发表日期	2009-09-01
发表期刊	Endocrinology 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Forkhead Box O1 (FoxO1) is a key transcription regulator of insulin/IGF-I signaling pathway, and its activity can be increased by dexamethasone (DEX) in several cell types. However, the role of FoxO1 in DEX-induced pancreatic β-cell dysfunction has not been fully understood. Therefore, in this study, we investigated whether FoxO1 could mediate DEX-induced β-cell dysfunction and the possible underlying mechanisms in pancreatic β-cell line RINm5F cells and primary rat islet. We found that DEX markedly increased FoxO1 mRNA and protein expression and decreased FoxO1 phosphorylation through the Akt pathway, which resulted in an increase in active FoxO1 in RINm5F cells and isolated rat islets. Activated FoxO1 subsequently inhibited pancreatic duodenal homeobox-1 expression and induced nuclear exclusion of pancreatic duodenal homeobox-1. Knockdown of FoxO1 by RNA interference restored the expression of pancreatic duodenal homeobox-1 and prevented DEX-induced dysfunction of glucose-stimulated insulin secretion in rat islets. Together, the results of present study demonstrate that FoxO1 is integrally involved in DEX-induced inhibition of pancreatic duodenal homeobox-1 and glucose-stimulated insulin secretion dysfunction in pancreatic islet β-cells. Inhibition of FoxO1 can effectively protect β-cells against DEX-induced dysfunction. Copyright © 2009 by The Endocrine Society.
ISSN	0013-7227
EISSN	0013-7227
卷号	150 期号:9 页码:4065-4073
DOI	10.1210/en.2009-0343
收录类别	SCOPUS
URL	查看原文
PubMed ID	19443572
SCOPUSEID	2-s2.0-69249139619
通讯作者地址	[Han, Xiao]Key Laboratory of Human Functional Genomics of Jiangsu Province,Jiangsu Diabetes Center,Nanjing Medical University,China
Scopus学科分类	Endocrinology
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/97578
专题	基础医学院（机能实验教学中心）_生物科学系_生物化学与分子生物学
通讯作者	Han, Xiao
作者单位	1.Key Laboratory of Human Functional Genomics of Jiangsu Province,Jiangsu Diabetes Center,Nanjing Medical University,China; 2.Department of Endocrinology,Jiangsu Diabetes Center,Nanjing Medical University,China; 3.Department of General Surgery,Nanjing Medical University,China; 4.Department of Biochemistry,Wenzhou Medical College,China
第一作者单位	基础医学院（机能实验教学中心）_生物科学系_生物化学与分子生物学
推荐引用方式 GB/T 7714	Zhang, Xiongfei,Yong, Wei,Lv, Jinghuan,et al. Inhibition of forkhead box O1 protects pancreatic β-cells against dexamethasone-induced dysfunction[J]. Endocrinology,2009,150(9):4065-4073.
APA	Zhang, Xiongfei., Yong, Wei., Lv, Jinghuan., Zhu, Yunxia., Zhang, Jingjing., ... & Han, Xiao. (2009). Inhibition of forkhead box O1 protects pancreatic β-cells against dexamethasone-induced dysfunction. Endocrinology, 150(9), 4065-4073.
MLA	Zhang, Xiongfei,et al."Inhibition of forkhead box O1 protects pancreatic β-cells against dexamethasone-induced dysfunction".Endocrinology 150.9(2009):4065-4073.