Early-Stage Primary Anti-inflammatory Therapy Enhances the Regenerative Efficacy of Platelet-Rich Plasma in a Rabbit Achilles Tendinopathy Model

doi:10.1177/03635465211037354

科研成果详情

题名	Early-Stage Primary Anti-inflammatory Therapy Enhances the Regenerative Efficacy of Platelet-Rich Plasma in a Rabbit Achilles Tendinopathy Model
作者	Ruan, Dengfeng 1; Fei, Yang ; Qian, Shengjun ; Huang, Zizhan ; Chen, Weishan 2,26,27; Tang, Chenqi 3,18,19,20,21,22; Xiang, Xinyu 4; Xu, Jialu 5; Yin, Zi 6,23,24,25; Chen, Xiao 7,13,14,15; Heng, Boon Chin 8; Liu, Wanlu 9,16,17; Shen, Weiliang 10; Ouyang, Hongwei 11
发表日期	2021-10
发表期刊	AMERICAN JOURNAL OF SPORTS MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	tendinopathy anti-inflammatory glucocorticoids platelet-rich plasma sequential therapy
其他关键词	ROTATOR CUFF REPAIR ; TENDON INJURY ; CELL-PROLIFERATION ; CLINICAL-OUTCOMES ; LEUKOCYTE ; TENOCYTES ; SCAFFOLD ; INFLAMMATION ; MECHANISMS ; INJECTIONS
摘要	Background: Tendinopathy is a pervasive clinical problem that afflicts both athletes and the general public. Although the inflammatory changes in tendinopathy are well characterized, how the therapeutic effects of platelet-rich plasma (PRP) on tendinopathy are being modulated by the inflammatory environment is not well defined. Purpose/Hypothesis: In this study, we aimed to compare the therapeutic effects of PRP alone versus a combination of PRP with a primary glucocorticoid (GC) injection at the early stage of tendinopathy. We hypothesized that PRP treatment could promote better tendon regeneration through the suppression of inflammation with GC. Study Design: Controlled laboratory study. Methods: The gene expression profile of tendon stem/progenitor cells (TSPCs) cultured with PRP was analyzed with RNA sequencing. To evaluate the cell viability, senescence, and apoptosis of TSPCs under different conditions, TSPCs were treated with 0.1 mg/mL triamcinolone acetonide (TA) and/or 10% PRP in an IL1B-induced inflammatory environment. To further verify the effects of the sequential therapy of GCs and PRP, an early tendinopathy animal model was established through a local injection of collagenase in the rabbit Achilles tendon. The tendinopathy model was then treated with isopycnic normal saline (NS group), TA (TA group), PRP (PRP group), or TA and PRP successively (TA+PRP group). At 8 weeks after treatment, the tendons were assessed with magnetic resonance imaging (MRI), histological examination, transmission electron microscopy (TEM), and mechanical testing. Results: Gene Ontology enrichment analysis indicated that PRP treatment of TPSCs induced an inflammatory response, regulated cell migration, and remodeled the extracellular matrix. Compared with the sole use of PRP, successive treatment with TA followed by PRP yielded similar results in cell viability and senescence but less cell apoptosis in vitro. In vivo experiments demonstrated that the TA+PRP group achieved significantly better tendon regeneration, as confirmed by MRI, histological examination, TEM, and mechanical testing. Conclusion: This study showed that the primary use of GCs did not exert any obvious deleterious side effects on the treated tendon but instead enhanced the regenerative effects of PRP in early inflammatory tendinopathy.
资助项目	National Natural Science Foundation of China[2017YFA0104900];
出版者	SAGE PUBLICATIONS INC
出版地	THOUSAND OAKS
ISSN	0363-5465
EISSN	1552-3365
卷号	49 期号:12 页码:3357-3371
DOI	10.1177/03635465211037354
页数	15
WOS类目	Orthopedics ; Sport Sciences
WOS研究方向	Orthopedics ; Sport Sciences
WOS记录号	WOS:000701731300001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	34549620
SCOPUSEID	2-s2.0-85115624623
通讯作者地址	[Shen, Weiliang]Department of Orthopedic Surgery,Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou,China ; [Ouyang, Hongwei]Dr Li Dak Sum Yip Yio Chin Center for Stem Cells and Regenerative Medicine,Zhejiang University School of Medicine,Department of Orthopedic Surgery,Hangzhou,China
Scopus学科分类	Physical Therapy, Sports Therapy and Rehabilitation;Orthopedics and Sports Medicine
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/8955
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_浙江省胰脏肝脏危重性疾病重点实验室
通讯作者	Shen, Weiliang; Ouyang, Hongwei
作者单位	1.Department of Orthopedic Surgery,Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou,China; 2.Department of Orthopedic Surgery,Hospital,Zhejiang University School of Medicine,Hangzhou,China; 3.Department of Orthopedic Surgery,Hospital,Zhejiang University School of Medicine,Hangzhou,China; 4.Zhejiang University–University of Edinburgh Institute,Zhejiang University School of Medicine,Haining,China; 5.Department of Infectious Diseases,First Affiliated Hospital,Wenzhou Medical University,Wenzhou,China; 6.Center for Stem Cells and Regenerative Medicine,Zhejiang University School of Medicine,Hangzhou,China; 7.Center for Stem Cells and Regenerative Medicine,Zhejiang University School of Medicine,Hangzhou,China; 8.Peking University School of Stomatology,Beijing,China; 9.Department of Orthopedic Surgery,Hospital,Zhejiang University School of Medicine,Hangzhou,China; 10.Department of Orthopedic Surgery,Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou,China; 11.Dr Li Dak Sum Yip Yio Chin Center for Stem Cells and Regenerative Medicine,Zhejiang University School of Medicine,Department of Orthopedic Surgery,Hangzhou,China; 12.Department of Orthopedic Surgery,Zhejiang University School of Medicine,Hangzhou,China; 13.Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province,Zhejiang University School of Medicine,Hangzhou,China; 14.China Orthopedic Regenerative Medicine Group,Hangzhou,China; 15.Zhejiang University–University of Edinburgh Institute,Zhejiang University School of Medicine,Haining,China; 16.Center for Stem Cells and Regenerative Medicine,Zhejiang University School of Medicine,Hangzhou,China; 17.Orthopedics Research Institute,Zhejiang University,Hangzhou,China; 18.Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province,Zhejiang University School of Medicine,Hangzhou,China; 19.Institute of Sports Medicine,Zhejiang University,Hangzhou,China; 20.Center for Stem Cells and Regenerative Medicine,Zhejiang University School of Medicine,Hangzhou,China; 21.Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province,Zhejiang University School of Medicine,Hangzhou,China; 22.Department of Orthopedic Surgery,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou,China; 23.Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province,Zhejiang University School of Medicine,Hangzhou,China; 24.China Orthopedic Regenerative Medicine Group,Hangzhou,China; 25.Orthopedics Research Institute,Zhejiang University,Hangzhou,China; 26.Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province,Zhejiang University School of Medicine,Hangzhou,China; 27.Institute of Sports Medicine,Zhejiang University,Hangzhou,China
推荐引用方式 GB/T 7714	Ruan, Dengfeng,Fei, Yang,Qian, Shengjun,et al. Early-Stage Primary Anti-inflammatory Therapy Enhances the Regenerative Efficacy of Platelet-Rich Plasma in a Rabbit Achilles Tendinopathy Model[J]. AMERICAN JOURNAL OF SPORTS MEDICINE,2021,49(12):3357-3371.
APA	Ruan, Dengfeng., Fei, Yang., Qian, Shengjun., Huang, Zizhan., Chen, Weishan., ... & Ouyang, Hongwei. (2021). Early-Stage Primary Anti-inflammatory Therapy Enhances the Regenerative Efficacy of Platelet-Rich Plasma in a Rabbit Achilles Tendinopathy Model. AMERICAN JOURNAL OF SPORTS MEDICINE, 49(12), 3357-3371.
MLA	Ruan, Dengfeng,et al."Early-Stage Primary Anti-inflammatory Therapy Enhances the Regenerative Efficacy of Platelet-Rich Plasma in a Rabbit Achilles Tendinopathy Model".AMERICAN JOURNAL OF SPORTS MEDICINE 49.12(2021):3357-3371.