葛根素对急性肺血栓栓塞溶栓治疗后再灌注损伤的影响

doi:10.3321/j.issn:1001-1978.2004.11.014

科研成果详情

题名	葛根素对急性肺血栓栓塞溶栓治疗后再灌注损伤的影响
其他题名	Effect of puerarin on reperfusion injury after thrombolytic therapy in acute pulmonary thromboembolism
作者	陈少贤 ; 姜琴华 ; 王良兴 ; 陈彦凡 ; 谢于鹏 ; 李焕斌 ; 张琦 ; 范小芳
发表日期	2004-11-30
发表期刊	中国药理学通报影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	葛根素肺血栓栓塞溶栓治疗再灌注损伤
摘要	目的　探讨葛根素对急性肺血栓栓塞溶栓治疗后再灌注损伤的影响及作用机制。方法　 2 4只日本大耳白兔 ,随机分为假手术组 (S组 )、单纯溶栓组 (T组 )、葛根素组 (Pur组 ) ,经右心导管注入条柱状自体血栓 ,心导管法监测血流动力学 ,制备急性肺血栓栓塞实验模型。测定栓塞前、栓塞后2h、溶栓后 2h、4h血浆中超氧化物歧化酶 (SOD)、丙二醛(MDA)的含量 ;术毕肺组织行病理及电镜观察。结果　①T组和Pur组在溶栓开始后 1h ,PAMP即显著下降 (P <0 0 1) ;在溶栓开始后 1hPur组PAMP即明显低于T组 (P<0 0 5 )。②在栓塞后 2h、溶栓后 2h、4h时 ,T组和Pur组血浆SOD活力均明显低于栓塞前 (P <0 0 5 ) ,而MDA含量在栓塞后 2h无明显变化 ,在溶栓后 2h、4h才开始明显升高 (P <0 0 5 ) ;与T组同期比较 ,Pur组在溶栓后 2h、4h血浆SOD活力和MDA含量分别有明显升高和下降 (P <0 0 5 ,P <0 0 1)。③组织病理及超微结构研究显示 ,Pur组血管内皮细胞和肺泡上皮细胞损伤较T组明显减轻。结论　葛根素对急性肺血栓栓塞溶栓后再灌注损伤有保护作用 ,其机制可能与其抗氧化损害的作用有关。
其他摘要	Aim To study the effect of puerarin on reper-fusion injury after thrornbolytic therapy in acute pulmo-nary thromboembolism and its mechanism. Methods Thirty-two Japanese rabbits were randomly divided into sham operation group ( group S) , Thrombolysis-only group (group T) , and Puerarin group (group Pur). A-cute pulmonary thromboembolism models of rabbits were established with injection of autologous blood clots through the right heart catheters, haemodynamic monito-ring was performed by introducing heart catheter through right jugular vein. The activity of plasma super oxide dismutase (SOD) and content of plasma malondi-aldehyde (MDA) were detected before embolization,2 h after embolization, 2 h and 4 h after thrombolysis. At the end, the rabbits were sacrificed and their lung, re-moved for histopathologic and electron microscopic in-vestigations. Results CD Pulmonary arterial mean pressure ( PAMP) were decreased at 1 hour after thrombolysis both in group T and group Pur (P < 0. 01) , PAMP in group Pur was significantly lower than those in group T at 1 h after thrombolysis (P <0. 05) . ? The activity of SOD began to decline at 2 h after embolization both in group T and group Pur (P<0.05), the content of MDA began to increase at 2 h after thromboloysis (P<0.05); At 2 h and 4h after throumbolysis, the activity of SOD was higher (P<0.05, P<0.01) and the content of MDA was lower (P<0.05, P<0.01) in group Pur than those in group T. ③ Histopathologic analysis showed the injuries of endothel and type II alvelolar cells in group T were more severe than those in group Pur. Conclusion Chinese herb puerarin can protect lung against reperfusion injury after thrompolytic therapy in acute pulmonary thrombembolism which may be associated with antioxidation action.
资助项目	浙江省中医药科技计划资助项目,No2004C125
ISSN	1001-1978
卷号	20 期号:11 页码:1245-1250
DOI	10.3321/j.issn:1001-1978.2004.11.014
页数	6
收录类别	CNKI ; CSCD ; 万方 ; 北大核心 ; PKU ; ISTIC
学科领域	医药、卫生 ; 中国医学 ; 基础医学
URL	查看原文
CSCD记录号	CSCD:1795959
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/87120
专题	附属第一医院附属第二医院_CT室
作者单位	1.温州医学院附属一院呼吸内科、肺心病研究室 2.温州医学院ECT室
推荐引用方式 GB/T 7714	陈少贤,姜琴华,王良兴,等. 葛根素对急性肺血栓栓塞溶栓治疗后再灌注损伤的影响[J]. 中国药理学通报,2004,20(11):1245-1250.
APA	陈少贤., 姜琴华., 王良兴., 陈彦凡., 谢于鹏., ... & 范小芳. (2004). 葛根素对急性肺血栓栓塞溶栓治疗后再灌注损伤的影响. 中国药理学通报, 20(11), 1245-1250.
MLA	陈少贤,et al."葛根素对急性肺血栓栓塞溶栓治疗后再灌注损伤的影响".中国药理学通报 20.11(2004):1245-1250.