A blood-based 22-gene expression signature for hepatocellular carcinoma identification

doi:10.21037/atm.2020.01.93

科研成果详情

题名	A blood-based 22-gene expression signature for hepatocellular carcinoma identification
作者	Zheng, Jie1; Zhu, Ming-Yu 2; Wu, Fei 3; Kang, Bin 3; Liang, Ji 3; Heskia, Fabienne 4; Shan, Yun-Feng5; Zhang, Xin-Xin 6,7
发表日期	2020-03
发表期刊	ANNALS OF TRANSLATIONAL MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Diagnosis carcinoma hepatocellular blood gene expression
其他关键词	CANCER ; THROMBOCYTOSIS ; PLATELETS ; DISEASE ; VIRUS
摘要	Background: Hepatocellular carcinoma (HCC) is one of the most common and lethal malignancies. Early detection of HCC could largely reduce mortalities. Ultrasonography (US) and serum Alpha Fetoprotein (AFP) test are the screening methods that are most frequently applied to high-risk populations. Due to the poor performance of AFP testing, and the highly operator-dependent nature of US, a biomarker for HCC early diagnosis is highly sought after. We developed a method for I ICC screening using a 22-gene expression signature. Methods: Peripheral whole blood of 98 patients were processed through microarrays for the first round of feature selection via two strategies, Minimal Redundancy Maximal Relevance and Least Absolute Shrinkage and Selection Operator combined with Support Vector Machine (SVM). Candidate genes were combined for further validation through qPCR in an enlarged population with 316 samples with 104 chronic hepatitis, 112 liver cirrhosis (LC), and 100 HCC. Results: A 22-gene signature was established in classifying HCC and non-cancer samples with good performance. The area under curve reached 0.94 in all of the samples and 0.93 in the AFP -negative samples. Conclusions: We have established a blood mRNA signature with high performance for HCC screening. Our results show transcriptome of peripheral blood could be valuable source for biomarkers.
出版者	AME PUBL CO
出版地	SHATIN
ISSN	2305-5839
EISSN	2305-5847
卷号	8 期号:5 页码:195
DOI	10.21037/atm.2020.01.93
页数	12
WOS类目	Oncology ; Medicine, Research & Experimental
WOS研究方向	Oncology ; Research & Experimental Medicine
WOS记录号	WOS:000520850100047
收录类别	SCIE ; PUBMED
URL	查看原文
PubMed ID	32309342
PMC记录号	PMC7154425
通讯作者地址	[Shan, Yun-Feng]Wenzhou Med Univ, Affiliated Hosp 1, Dept Surg, Wenzhou 325000, Peoples R China. ; [Zhang, Xin-Xin]Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Res Lab Clin Virol, Shanghai 200025, Peoples R China. ; [Zhang, Xin-Xin]Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp North, Shanghai 200025, Peoples R China.
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/8354
专题	第一临床医学院（信息与工程学院）、附属第一医院_影像医学与核医学_介入科附属第一医院
通讯作者	Shan, Yun-Feng; Zhang, Xin-Xin
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 1, Dept Intervent Radiol, Wenzhou 325000, Peoples R China; 2.Shanghai Jiao Tong Univ, Ruijin Hosp North, Sch Med, Dept Gastroenterol, Shanghai 201800, Peoples R China; 3.Fudan Univ, Canc Inst, Inst Merieux Lab, Shanghai Canc Ctr, Shanghai 200032, Peoples R China; 4.BioMerieux, Med Diagnost Discovery Dept, Marcy Letoile, France; 5.Wenzhou Med Univ, Affiliated Hosp 1, Dept Surg, Wenzhou 325000, Peoples R China; 6.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Res Lab Clin Virol, Shanghai 200025, Peoples R China; 7.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp North, Shanghai 200025, Peoples R China
第一作者单位	第一临床医学院（信息与工程学院）、附属第一医院_影像医学与核医学_介入科
通讯作者单位	附属第一医院
第一作者的第一单位	第一临床医学院（信息与工程学院）、附属第一医院_影像医学与核医学_介入科
推荐引用方式 GB/T 7714	Zheng, Jie,Zhu, Ming-Yu,Wu, Fei,et al. A blood-based 22-gene expression signature for hepatocellular carcinoma identification[J]. ANNALS OF TRANSLATIONAL MEDICINE,2020,8(5):195.
APA	Zheng, Jie., Zhu, Ming-Yu., Wu, Fei., Kang, Bin., Liang, Ji., ... & Zhang, Xin-Xin. (2020). A blood-based 22-gene expression signature for hepatocellular carcinoma identification. ANNALS OF TRANSLATIONAL MEDICINE, 8(5), 195.
MLA	Zheng, Jie,et al."A blood-based 22-gene expression signature for hepatocellular carcinoma identification".ANNALS OF TRANSLATIONAL MEDICINE 8.5(2020):195.