依达拉奉对惊厥持续状态大鼠海马IRE1 mRNA表达及神经元凋亡的影响

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题名	依达拉奉对惊厥持续状态大鼠海马IRE1 mRNA表达及神经元凋亡的影响
其他题名	Effects of edaravone on IRE1 mRNA expression and neuronal apoptosis in the hippo-campus of rats with status convulsivus
作者	焦颖; 邓小龙; 李光乾
发表日期	2009-06-15
发表期刊	中国当代儿科杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	依达拉奉惊厥持续状态 IRE1 神经元凋亡内质网应激大鼠
摘要	目的探讨依达拉奉(EDA)对惊厥持续状态(SC)后大鼠海马内质网应激关键标志分子IRE1表达及神经元凋亡的影响。方法将19～21d日龄的Sprague-Dawley大鼠随机分为SC组、EDA组、对照组。其中SC组、EDA组再按SC后处死时间点不同分别分为4,12,24,48,72h5个亚组;每组均8只。用RT-PCR检测SC后海马IRE1mRNA的表达。用原位细胞凋亡检测法(TUNEL)观察神经元凋亡细胞数。电镜观察海马CA1区神经元超微结构变化。结果①RT-PCR结果:SC组4h和12h时间点IRE1mRNA含量较对照组明显升高,差异有非常显著性(P<0.01);EDA组4,12,24h时间点IRE1mRNA含量显著高于SC组对应时间点及对照组的含量,差异有非常显著性(P<0.01)。②TUNEL结果:SC组12～72h时间点TUNEL阳性细胞数多于对照组,差异有显著性(P<0.01),且SC组TUNEL阳性细胞数随惊厥持续时间延长而增加。EDA组12～72h时间点TUNEL阳性细胞数较SC组明显减少,差异有显著性(P<0.05或P<0.01),但仍高于对照组,差异有显著性(P<0.05或P<0.01)。③电镜观察结果:SC组及EDA组4h后即开始出现核周细胞器的改变,12h后出现明显细胞核的改变。其中SC组细胞凋亡严重,EDA组凋亡较轻。结论EDA可能通过上调IRE1的表达及维持其活性而缓解内质网压力而发挥对神经元的保护作用,有望成为在体抑制内质网应激的有效药物。
其他摘要	Objective To investigate the expression of the key marker of endoplasmic reticulum stress ( ERS) IRE1 mKNA and neuronal apoptosis in the rat hippocampus after status convulsivus ( SC), and the intervention effects of edaravone, a novel free radical scavenger. Methods Sprague-Dawley (SD) rats aged 19-21 days were randomly assigned to three groups: normal control, SC and edaravone-treated SC. SC was induced in the later two groups. The two groups were subdivided into 5 groups sacrificed at 4, 12, 24, 48, and 72 hrs after SC induction. IRE1 mRNA expression in the hippocampus was detected by RT-PCR. Neuronal apoptosis was observed by TdT-mediated dUTP nick end labeling (TUNEL) . The ultramicrostructural changes of neuron were observed by electron microscopy. Results IRE1 mRNA expression was obviously up-regulated 4 and 12 hrs after SC compared with the normal control group (P < 0. 01). IRE1 mRNA expression in the edaravone-treated SC group was notably higher than the untreated SC group 4, 12 and 24 hrs after SC and the normal control group (P < 0. 01). TUNEL positive cells in the hippocampus in the untreated SC group were significantly more than those in the normal control group ( P <0.01). The number of TUNEL positive cells increased with the prolonged convulsion time. TUNEL positive cells in the edaravone-treated SC group were significantly reduced compared with those in the untreated SC group 12, 24, 48 and 72 hrs after SC (P <0.05 or P <0.01), but remained higher than the normal control group (P <0.05 or P <0. 01). The peri-nucleus cell organ injuries were observed 4 hrs after SC and karyopyenosis and cytoplasm condensation were observed 12 hrs after SC in the SC and the edaravone-treated SC groups. The edaravone-treated SC group demonstrated less severe apoptosis than the untreated SC group. Conclusions Edaravone may have neuroprotections against SC by an up-regulation of IRE1 expression. It might serve as an effective agent for reducing ERS in vivo.
ISSN	1008-8830
卷号	11 期号:06 页码:471-475
页数	5
收录类别	CNKI ; 万方 ; 北大核心 ; PKU ; CSCD ; ISTIC
学科领域	医药、卫生
URL	查看原文
CSCD记录号	CSCD:3525503
CNKI分类号	R742.1
万方分类号	R-33(实验医学、医学实验)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/78567
专题	第二临床医学院，附属第二医院、育英儿童医院_儿科学_儿内神经
作者单位	温州医学院附属育英儿童医院小儿神经内科
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院_儿科学_儿内神经
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院_儿科学_儿内神经
推荐引用方式 GB/T 7714	焦颖,邓小龙,李光乾. 依达拉奉对惊厥持续状态大鼠海马IRE1 mRNA表达及神经元凋亡的影响[J]. 中国当代儿科杂志,2009,11(06):471-475.
APA	焦颖, 邓小龙, & 李光乾. (2009). 依达拉奉对惊厥持续状态大鼠海马IRE1 mRNA表达及神经元凋亡的影响. 中国当代儿科杂志, 11(06), 471-475.
MLA	焦颖,et al."依达拉奉对惊厥持续状态大鼠海马IRE1 mRNA表达及神经元凋亡的影响".中国当代儿科杂志 11.06(2009):471-475.