Assessing the drug resistance profiles of oral probiotic lozenges

doi:10.1080/20002297.2021.2019992

科研成果详情

题名	Assessing the drug resistance profiles of oral probiotic lozenges
作者	Wang, Yi1; Dong, Jingya1; Wang, Junyi 2; Chi, Wei 2; Zhou, Wei 2; Tian, Qiwen 2; Hong, Yue 1; Zhou, Xuan 2; Ye, Hailv 2; Tian, Xuechen 3,4; Hu, Rongdang1; Wong, Aloysius 2,3,4
发表日期	2022-12-31
发表期刊	JOURNAL OF ORAL MICROBIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Probiotics antibiotic resistance probiotic supplements oral probiotics probiotic lozenges dental probiotics lactobacillus horizontal gene transfer
其他关键词	LACTIC-ACID BACTERIA ; ANTIBIOTIC-RESISTANCE ; CONJUGAL TRANSFER ; TETRACYCLINE RESISTANCE ; VANCOMYCIN RESISTANCE ; GENE-TRANSFER ; HUMAN-ORIGIN ; MOLECULAR CHARACTERIZATION ; ANTIMICROBIAL RESISTANCE ; ERYTHROMYCIN-RESISTANT
摘要	Background Probiotic lozenges have been developed to harvest the benefits of probiotics for oral health, but their long-term consumption may encourage the transfer of resistance genes from probiotics to commensals, and eventually to disease-causing bacteria. Aim To screen commercial probiotic lozenges for resistance to antibiotics, characterize the resistance determinants, and examine their transferability in vitro. Results Probiotics of all lozenges were resistant to glycopeptide, sulfonamide, and penicillin antibiotics, while some were resistant to aminoglycosides and cephalosporins. High minimum inhibitory concentrations (MICs) were detected for streptomycin (>128 mu g/mL) and chloramphenicol (> 512 mu g/mL) for all probiotics but only one was resistant to piperacillin (MIC = 32 mu g/mL). PCR analysis detected erythromycin (erm(T), ermB or mefA) and fluoroquinolone (parC or gyr(A)) resistance genes in some lozenges although there were no resistant phenotypes. The dfrD, cat-TC, vatE, aadE, vanX, and aph(3)-III or ant(2)-I genes conferring resistance to trimethoprim, chloramphenicol, quinupristin/dalfopristin, vancomycin, and streptomycin, respectively, were detected in resistant probiotics. The rifampicin resistance gene rpoB was also present. We found no conjugal transfer of streptomycin resistance genes in our co-incubation experiments. Conclusion Our study represents the first antibiotic resistance profiling of probiotics from oral lozenges, thus highlighting the health risk especially in the prevailing threat of drug resistance globally.
资助项目	Wenzhou-Kean University under the Student Partnering with Faculty (SpF) research program [WKU201920009, SpF2021002]; Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LQ20H140002]; Wenzhou Science and Technology bureau [Y20190100]
出版者	TAYLOR & FRANCIS LTD
出版地	ABINGDON
ISSN	2000-2297
EISSN	2000-2297
卷号	14 期号:1 页码:2019992
DOI	10.1080/20002297.2021.2019992
页数	20
WOS类目	Microbiology
WOS研究方向	Microbiology
WOS记录号	WOS:000740042700001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	35024089
PMC记录号	PMC8745366
SCOPUSEID	2-s2.0-85122470350
通讯作者地址	[Hu, Rongdang]Department of Orthodontics,School and Hospital of Stomatology,Wenzhou Medical University,University Town,Wenzhou, Zhejiang Province,Chashan,325060,China
Scopus学科分类	Dentistry (miscellaneous);Microbiology (medical);Infectious Diseases
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7784
专题	口腔医学院、附属口腔医院
通讯作者	Hu, Rongdang
作者单位	1.Department of Orthodontics,School and Hospital of Stomatology,Wenzhou Medical University,University Town,Zhejiang Province,Wenzhou,China; 2.Department of Biology,College of Science and Technology,Wenzhou-Kean University,Zhejiang Province,Wenzhou,China; 3.Zhejiang Bioinformatics International Science and Technology Cooperation Center,Zhejiang Province,Wenzhou,China; 4.Wenzhou Municipal Key Lab for Applied Biomedical and Biopharmaceutical Informatics,Zhejiang Province,Wenzhou,China
第一作者单位	口腔医学院、附属口腔医院
通讯作者单位	口腔医学院、附属口腔医院
第一作者的第一单位	口腔医学院、附属口腔医院
推荐引用方式 GB/T 7714	Wang, Yi,Dong, Jingya,Wang, Junyi,et al. Assessing the drug resistance profiles of oral probiotic lozenges[J]. JOURNAL OF ORAL MICROBIOLOGY,2022,14(1):2019992.
APA	Wang, Yi., Dong, Jingya., Wang, Junyi., Chi, Wei., Zhou, Wei., ... & Wong, Aloysius. (2022). Assessing the drug resistance profiles of oral probiotic lozenges. JOURNAL OF ORAL MICROBIOLOGY, 14(1), 2019992.
MLA	Wang, Yi,et al."Assessing the drug resistance profiles of oral probiotic lozenges".JOURNAL OF ORAL MICROBIOLOGY 14.1(2022):2019992.