The ETS-Domain Transcription Factor Elk-1 Regulates COX-2 Gene Expression and Inhibits Glucose-Stimulated Insulin Secretion in the Pancreatic beta-Cell Line INS-1

doi:10.1155/2013/843462

科研成果详情

题名	The ETS-Domain Transcription Factor Elk-1 Regulates COX-2 Gene Expression and Inhibits Glucose-Stimulated Insulin Secretion in the Pancreatic beta-Cell Line INS-1
作者	Zhang, Xiong-Fei1; Zhu, Yi 2,3; Liang, Wen-Biao 4; Zhang, Jing-Jing 2,3
发表日期	2013-12-31
发表期刊	INTERNATIONAL JOURNAL OF ENDOCRINOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	CYCLOOXYGENASE-2 PROMOTER ACTIVITY ; SODIUM-SALICYLATE ; KAPPA-B ; GROWTH ; FAMILY ; INVOLVEMENT ; CRE
摘要	Cyclooxygenase-2 (COX-2) expression is associated with many aspects of physiological and pathological conditions, including pancreatic beta-cell dysfunction. Prostaglandin E2 (PGE2) production, as a consequence of COX-2 gene induction, has been reported to impair beta-cell function. The molecular mechanisms involved in the regulation of COX-2 gene expression are not fully understood. We previously demonstrated that transcription factor Elk-1 significantly upregulated COX-2 gene promoter activity. In this report, we used pancreatic beta-cell line (INS-1) to explore the relationships between Elk-1 and COX-2. We first investigated the effects of Elk-1 on COX-2 transcriptional regulation and expression in INS-1 cells. We thus undertook to study the binding of Elk-1 to its putative binding sites in the COX-2 promoter. We also analysed glucose-stimulated insulin secretion (GSIS) in INS-1 cells that overexpressed Elk-1. Our results demonstrate that Elk-1 efficiently upregulates COX-2 expression at least partly through directly binding to the -82/-69 region of COX-2 promoter. Overexpression of Elk-1 inhibits GSIS in INS-1 cells. These findings will be helpful for better understanding the transcriptional regulation of COX-2 in pancreatic beta-cell. Moreover, Elk-1, the transcriptional regulator of COX-2 expression, will be a potential target for the prevention of beta-cell dysfunction mediated by PGE2.
资助项目	Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [Y2110310]; Science and Technology Project of Wenzhou City [Y20100243]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81001079, 81101802]; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) [JX10231801]
出版者	HINDAWI LTD
出版地	LONDON
ISSN	1687-8337
EISSN	1687-8345
卷号	2013 页码:843462
DOI	10.1155/2013/843462
页数	8
WOS类目	Endocrinology & Metabolism
WOS研究方向	Endocrinology & Metabolism
WOS记录号	WOS:000320519700001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	23818898
PMC记录号	PMC3684088
SCOPUSEID	2-s2.0-84879307977
通讯作者地址	[Zhang, Jing-Jing]Department of General Surgery,First Affiliated Hospital of Nanjing Medical University,300 Guangzhou Road,China
Scopus学科分类	Endocrinology, Diabetes and Metabolism;Endocrinology;Endocrine and Autonomic Systems
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7708
专题	基础医学院（机能实验教学中心）_生物科学系_生物化学与分子生物学
通讯作者	Zhang, Jing-Jing
作者单位	1.Department of Biochemistry,Wenzhou Medical College,China; 2.Department of General Surgery,First Affiliated Hospital of Nanjing Medical University,300 Guangzhou Road,China; 3.Jiangsu Province Academy of Clinical Medicine,Institute of Tumor Biology,300 Guangzhou Road,China; 4.Transfusion Laboratory,Jiangsu Province Blood Center,China
第一作者单位	基础医学院（机能实验教学中心）_生物科学系_生物化学与分子生物学
第一作者的第一单位	基础医学院（机能实验教学中心）_生物科学系_生物化学与分子生物学
推荐引用方式 GB/T 7714	Zhang, Xiong-Fei,Zhu, Yi,Liang, Wen-Biao,et al. The ETS-Domain Transcription Factor Elk-1 Regulates COX-2 Gene Expression and Inhibits Glucose-Stimulated Insulin Secretion in the Pancreatic beta-Cell Line INS-1[J]. INTERNATIONAL JOURNAL OF ENDOCRINOLOGY,2013,2013:843462.
APA	Zhang, Xiong-Fei, Zhu, Yi, Liang, Wen-Biao, & Zhang, Jing-Jing. (2013). The ETS-Domain Transcription Factor Elk-1 Regulates COX-2 Gene Expression and Inhibits Glucose-Stimulated Insulin Secretion in the Pancreatic beta-Cell Line INS-1. INTERNATIONAL JOURNAL OF ENDOCRINOLOGY, 2013, 843462.
MLA	Zhang, Xiong-Fei,et al."The ETS-Domain Transcription Factor Elk-1 Regulates COX-2 Gene Expression and Inhibits Glucose-Stimulated Insulin Secretion in the Pancreatic beta-Cell Line INS-1".INTERNATIONAL JOURNAL OF ENDOCRINOLOGY 2013(2013):843462.