题名 | Dual Delivery of NGF and bFGF Coacervater Ameliorates Diabetic Peripheral Neuropathy via Inhibiting Schwann Cells Apoptosis |
作者 | |
发表日期 | 2017-12-31 |
发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
关键词 | diabetic peripheral neuropathy (DPN) schwann cells (SCs) basic fibroblast growth factor (bFGF) nerve growth factor (NGF) coacervate |
其他关键词 | ENDOPLASMIC-RETICULUM STRESS ; NERVE GROWTH-FACTOR ; HEPARIN-BASED COACERVATE ; PROLIFERATION ; REGENERATION ; RATS ; PATHOGENESIS ; INDUCTION ; MELLITUS ; AXONS |
摘要 | Diabetic neuropathy is a kind of insidious complications that impairs neural and vascular function and ultimately leads to somatic and visceral denervation. Basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) are important neurotrophic factors for stimulating angiogenesis and improving peripheral nerve function. Administrating a single factor has good therapeutic effect on diabetic peripheral neuropathy (DPN). However, the short half-life and rapid diffusion of growth factors under physiological conditions limits its clinical applications. Here, we used a biodegradable coacervate, composed of heparin and polycation, to dominate the combined release of bFGF and NGF in a steady fashion. We found this combined growth factors (GFs) coacervate, administered as a single injection, improved motor and sensory functions, restored morphometric structure and decreased apoptosis of Schwann cells in a rat model of prolonged DPN. Similarly the GFs coacervate, as compared with free bFGF and NGF combination, markedly reduced the apoptosis level of a rat Schwann cell line, RSC 96 cells in vitro. We also demonstrated that neuroprotective effects of the GFs coacervate in both rat DPN model and hyperglycemia-induced RSC 96 cell model is likely due to suppression of endocytoplasmic reticulum stress (ERS). |
资助项目 | National Natural Science Funding of ChinaNational Natural Science Foundation of China (NSFC) [81372112]; Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LQ15E030003]; Wenzhou Municipal Science & Technology Bureau of China [Y20140574] |
出版者 | IVYSPRING INT PUBL |
出版地 | LAKE HAVEN |
ISSN | 1449-2288 |
卷号 | 13期号:5页码:640-651 |
DOI | 10.7150/ijbs.18636 |
页数 | 12 |
WOS类目 | Biochemistry & Molecular Biology ; Biology |
WOS研究方向 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics |
WOS记录号 | WOS:000401904400009 |
收录类别 | SCIE ; PUBMED ; SCOPUS |
URL | 查看原文 |
PubMed ID | 28539836 |
PMC记录号 | PMC5441180 |
SCOPUSEID | 2-s2.0-85019557104 |
通讯作者地址 | [Xiao, Jian]WZMU-JCU Joint Research Group for Stem Cell and Tissue Engineering,Institute of Stem Cell and Tissue Engineering,School of Pharmacy,Wenzhou Medical University,Wenzhou,325035,China |
Scopus学科分类 | Ecology, Evolution, Behavior and Systematics;Applied Microbiology and Biotechnology;Molecular Biology;Developmental Biology;Cell Biology |
TOP期刊 | TOP期刊 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/7324 |
专题 | 口腔医学院、附属口腔医院_干细胞与组织工程研究所 药学院(分析测试中心) 附属第一医院 |
通讯作者 | Xiao, Jian |
作者单位 | 1.WZMU-JCU Joint Research Group for Stem Cell and Tissue Engineering,Institute of Stem Cell and Tissue Engineering,School of Pharmacy,Wenzhou Medical University,Wenzhou,325035,China; 2.UQ-WMU Joint Research Group for Regenerative Medicine,Oral Health Centre,University of Queensland,Brisbane,4006,Australia; 3.The Institute of Life Sciences,Wenzhou University,Wenzhou,325035,China; 4.Department of Peripheral Neurosurgery,The First Affiliated Hospital Wenzhou Medical University,Wenzhou,325035,China |
第一作者单位 | 药学院(分析测试中心) |
通讯作者单位 | 药学院(分析测试中心) |
第一作者的第一单位 | 药学院(分析测试中心) |
推荐引用方式 GB/T 7714 | Li, Rui,Ma, Jianfeng,Wu, Yanqing,et al. Dual Delivery of NGF and bFGF Coacervater Ameliorates Diabetic Peripheral Neuropathy via Inhibiting Schwann Cells Apoptosis[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2017,13(5):640-651. |
APA | Li, Rui., Ma, Jianfeng., Wu, Yanqing., Nangle, Matthew., Zou, Shuang., ... & Xiao, Jian. (2017). Dual Delivery of NGF and bFGF Coacervater Ameliorates Diabetic Peripheral Neuropathy via Inhibiting Schwann Cells Apoptosis. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 13(5), 640-651. |
MLA | Li, Rui,et al."Dual Delivery of NGF and bFGF Coacervater Ameliorates Diabetic Peripheral Neuropathy via Inhibiting Schwann Cells Apoptosis".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 13.5(2017):640-651. |
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