Dual Delivery of NGF and bFGF Coacervater Ameliorates Diabetic Peripheral Neuropathy via Inhibiting Schwann Cells Apoptosis

doi:10.7150/ijbs.18636

科研成果详情

题名	Dual Delivery of NGF and bFGF Coacervater Ameliorates Diabetic Peripheral Neuropathy via Inhibiting Schwann Cells Apoptosis
作者	Li, Rui 1; Ma, Jianfeng 1,2; Wu, Yanqing 3; Nangle, Matthew 2; Zou, Shuang 1; Li, Yiyang1; Yin, Jiayu 1; Zhao, Yingzheng1; Xu, Helin1; Zhang, Hongyu1; Li, Xiaokun 3; Ye, Qing Song 1,2; Wang, Jian4; Xiao, Jian 1
发表日期	2017-12-31
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	diabetic peripheral neuropathy (DPN) schwann cells (SCs) basic fibroblast growth factor (bFGF) nerve growth factor (NGF) coacervate
其他关键词	ENDOPLASMIC-RETICULUM STRESS ; NERVE GROWTH-FACTOR ; HEPARIN-BASED COACERVATE ; PROLIFERATION ; REGENERATION ; RATS ; PATHOGENESIS ; INDUCTION ; MELLITUS ; AXONS
摘要	Diabetic neuropathy is a kind of insidious complications that impairs neural and vascular function and ultimately leads to somatic and visceral denervation. Basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) are important neurotrophic factors for stimulating angiogenesis and improving peripheral nerve function. Administrating a single factor has good therapeutic effect on diabetic peripheral neuropathy (DPN). However, the short half-life and rapid diffusion of growth factors under physiological conditions limits its clinical applications. Here, we used a biodegradable coacervate, composed of heparin and polycation, to dominate the combined release of bFGF and NGF in a steady fashion. We found this combined growth factors (GFs) coacervate, administered as a single injection, improved motor and sensory functions, restored morphometric structure and decreased apoptosis of Schwann cells in a rat model of prolonged DPN. Similarly the GFs coacervate, as compared with free bFGF and NGF combination, markedly reduced the apoptosis level of a rat Schwann cell line, RSC 96 cells in vitro. We also demonstrated that neuroprotective effects of the GFs coacervate in both rat DPN model and hyperglycemia-induced RSC 96 cell model is likely due to suppression of endocytoplasmic reticulum stress (ERS).
资助项目	National Natural Science Funding of ChinaNational Natural Science Foundation of China (NSFC) [81372112]; Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LQ15E030003]; Wenzhou Municipal Science & Technology Bureau of China [Y20140574]
出版者	IVYSPRING INT PUBL
出版地	LAKE HAVEN
ISSN	1449-2288
卷号	13 期号:5 页码:640-651
DOI	10.7150/ijbs.18636
页数	12
WOS类目	Biochemistry & Molecular Biology ; Biology
WOS研究方向	Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS记录号	WOS:000401904400009
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28539836
PMC记录号	PMC5441180
SCOPUSEID	2-s2.0-85019557104
通讯作者地址	[Xiao, Jian]WZMU-JCU Joint Research Group for Stem Cell and Tissue Engineering,Institute of Stem Cell and Tissue Engineering,School of Pharmacy,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类	Ecology, Evolution, Behavior and Systematics;Applied Microbiology and Biotechnology;Molecular Biology;Developmental Biology;Cell Biology
引用统计	被引频次：32[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7324
专题	口腔医学院、附属口腔医院_干细胞与组织工程研究所附属第一医院
通讯作者	Xiao, Jian
作者单位	1.WZMU-JCU Joint Research Group for Stem Cell and Tissue Engineering,Institute of Stem Cell and Tissue Engineering,School of Pharmacy,Wenzhou Medical University,Wenzhou,325035,China; 2.UQ-WMU Joint Research Group for Regenerative Medicine,Oral Health Centre,University of Queensland,Brisbane,4006,Australia; 3.The Institute of Life Sciences,Wenzhou University,Wenzhou,325035,China; 4.Department of Peripheral Neurosurgery,The First Affiliated Hospital Wenzhou Medical University,Wenzhou,325035,China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Li, Rui,Ma, Jianfeng,Wu, Yanqing,et al. Dual Delivery of NGF and bFGF Coacervater Ameliorates Diabetic Peripheral Neuropathy via Inhibiting Schwann Cells Apoptosis[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2017,13(5):640-651.
APA	Li, Rui., Ma, Jianfeng., Wu, Yanqing., Nangle, Matthew., Zou, Shuang., ... & Xiao, Jian. (2017). Dual Delivery of NGF and bFGF Coacervater Ameliorates Diabetic Peripheral Neuropathy via Inhibiting Schwann Cells Apoptosis. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 13(5), 640-651.
MLA	Li, Rui,et al."Dual Delivery of NGF and bFGF Coacervater Ameliorates Diabetic Peripheral Neuropathy via Inhibiting Schwann Cells Apoptosis".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 13.5(2017):640-651.