The Anti-Scar Effects of Basic Fibroblast Growth Factor on the Wound Repair In Vitro and In Vivo

doi:10.1371/journal.pone.0059966

科研成果详情

题名	The Anti-Scar Effects of Basic Fibroblast Growth Factor on the Wound Repair In Vitro and In Vivo
作者	Shi, Hong-Xue1; Lin, Cai2; Lin, Bei-Bei1; Wang, Zhou-Guang 1; Zhang, Hong-Yu1; Wu, Fen-Zan1; Cheng, Yi 1; Xiang, Li-Jun 1; Guo, Di-Jiong 3; Luo, Xu2; Zhang, Guo-You4; Fu, Xiao-Bing 5; Bellusci, Saverio 6; Li, Xiao-Kun1; Xiao, Jian1
发表日期	2013-04-02
发表期刊	PLOS ONE 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	SMOOTH MUSCLE ACTIN ; RABBIT EAR MODEL ; HYPERTROPHIC SCARS ; FACTOR-BETA ; TEMPORAL EXPRESSION ; DERMAL FIBROBLASTS ; APOPTOSIS ; TISSUE ; MECHANISMS ; BIOLOGY
摘要	Hypertrophic scars (HTS) and keloids are challenging problems. Their pathogenesis results from an overproduction of fibroblasts and excessive deposition of collagen. Studies suggest a possible anti-scarring effect of basic fibroblast growth factor (bFGF) during wound healing, but the precise mechanisms of bFGF are still unclear. In view of this, we investigated the therapeutic effects of bFGF on HTS animal model as well as human scar fibroblasts (HSF) model. We show that bFGF promoted wound healing and reduced the area of flattened non-pathological scars in rat skin wounds and HTS in the rabbit ear. We provide evidence of a new therapeutic strategy: bFGF administration for the treatment of HTS. The scar elevation index (SEI) and epidermal thickness index (ETI) was also significantly reduced. Histological reveal that bFGF exhibited significant amelioration of the collagen tissue. bFGF regulated extracellular matrix (ECM) synthesis and degradation via interference in the collagen distribution, the alpha-smooth muscle actin (alpha-SMA) and transforming growth factor-1 (TGF-beta 1) expression. In addition, bFGF reduced scarring and promoted wound healing by inhibiting TGF beta 1/SMAD-dependent pathway. The levels of fibronectin (FN), tissue inhibitor of metalloproteinase-1 (TIMP-1) collagen I, and collagen III were evidently decreased, and matrix metalloproteinase-1 (MMP-1) and apoptosis cells were markedly increased. These results suggest that bFGF possesses favorable therapeutic effects on hypertrophic scars in vitro and in vivo, which may be an effective cure for human hypertrophic scars.
资助项目	Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents; State Key Basic Research Development Program [2012CB518105]; Zhejiang Provincial Project of Key Group [2010R50042]; Ninbo City Natural Science Foundation [R2090550]; Zhejiang Natural Science FoundationNatural Science Foundation of Zhejiang Province [Z2080985]
出版者	PUBLIC LIBRARY SCIENCE
出版地	SAN FRANCISCO
ISSN	1932-6203
卷号	8 期号:4 页码:e59966.
DOI	10.1371/journal.pone.0059966
页数	12
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000317717300047
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	23565178
PMC记录号	PMC3615060
SCOPUSEID	2-s2.0-84875674654
通讯作者地址	[Li, Xiao-Kun]Wenzhou Med Coll, Sch Pharm, Key Lab Biotechnol & Pharmaceut Engn, Wenzhou, Peoples R China.
引用统计	被引频次：132[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7093
专题	药学院（分析测试中心）附属第二医院第二临床医学院、附属第二医院、育英儿童医院附属第一医院_烧伤科其他_附属慈溪医院（慈溪市人民医院）
通讯作者	Li, Xiao-Kun
作者单位	1.Wenzhou Med Coll, Sch Pharm, Key Lab Biotechnol & Pharmaceut Engn, Wenzhou, Peoples R China; 2.Wenzhou Med Coll, Dept Burns, Affiliated Hosp 1, Wenzhou, Peoples R China; 3.Wenzhou Med Coll, Cixi Hosp, Dept Gen Surg, Ningbo, Zhejiang, Peoples R China; 4.Wenzhou Med Coll, Dept Orthopaed, Affiliated Hosp 2, Wenzhou, Peoples R China; 5.Chinese Peoples Liberat Army Gen Hosp, Inst Basic Med Sci, Wound Healing & Cell Biol Lab, Beijing, Peoples R China; 6.Univ Giessen, German Ctr Lung Res, D-35390 Giessen, Hessen, Germany
第一作者单位	药学院（分析测试中心）
通讯作者单位	药学院（分析测试中心）
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Shi, Hong-Xue,Lin, Cai,Lin, Bei-Bei,et al. The Anti-Scar Effects of Basic Fibroblast Growth Factor on the Wound Repair In Vitro and In Vivo[J]. PLOS ONE,2013,8(4):e59966..
APA	Shi, Hong-Xue., Lin, Cai., Lin, Bei-Bei., Wang, Zhou-Guang., Zhang, Hong-Yu., ... & Xiao, Jian. (2013). The Anti-Scar Effects of Basic Fibroblast Growth Factor on the Wound Repair In Vitro and In Vivo. PLOS ONE, 8(4), e59966..
MLA	Shi, Hong-Xue,et al."The Anti-Scar Effects of Basic Fibroblast Growth Factor on the Wound Repair In Vitro and In Vivo".PLOS ONE 8.4(2013):e59966..