TBBPA exposure during a sensitive developmental window produces neurobehavioral changes in larval zebrafish

doi:10.1016/j.envpol.2016.05.059

科研成果详情

题名	TBBPA exposure during a sensitive developmental window produces neurobehavioral changes in larval zebrafish
作者	Chen, Jiangfei1,2; Tanguay, Robert L.3; Xiao, Yanyan 2; Haggard, Derik E.3; Ge, Xiaoqing 2; Jia, Yinhang 2; Zheng, Yi 2; Dong, Qiaoxiang2; Huang, Changjiang 2; Lin, Kuangfei 1
发表日期	2016-09
发表期刊	ENVIRONMENTAL POLLUTION 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Zebrafish TBBPA Sensitive window Neurotoxicity Motor behavior ugt expression
其他关键词	BROMINATED FLAME RETARDANTS ; TETRABROMOBISPHENOL-A TBBPA ; DANIO-RERIO ; BISPHENOL-A ; TOXICITY ; OLIGODENDROCYTE ; METABOLISM ; APOPTOSIS ; NEURONS ; BDE-47
摘要	Tetrabromobisphenol A (TBBPA), one of the most widely used brominated flame retardants (BFRs), is a ubiquitous contaminant in the environment and in the human body. This study demonstrated that zebrafish embryos exposed to TBBPA during a sensitive window of 8-48 h post-fertilization (hpf) displayed morphological malformations and mortality. Zebrafish exposed exclusively between 48 and 96 hpf were phenotypically normal. TBBPA was efficiently absorbed and accumulated in zebrafish embryos, but was eliminated quickly when the exposure solution was removed. Larval behavior assays conducted at 120 hpf indicated that exposure to 5 mu M TBBPA from 8 to 48 hpf produced larvae with significantly lower average activity and speed of movement in the normal condition than in those exposed from 48 to 96 hpf. Specifically, 8-48 hpf-exposed larvae spent significantly less time in both activity bursts and gross movements compared to control or 48-96 hpf exposed larvae. Consistent with the motor deficits, TBBPA induced apoptotic cell death, delayed cranial motor neuron development, inhibited, primary motor neuron development and loosed muscle fiber during the early developmental stages. To further explore TBBPA-induced developmental and neurobehavioral toxicity, RNA-Seq analysis was used to identify early transcriptional changes following TBBPA exposure. In total, 1969 transcripts were significantly differentially expressed (P < 0.05, FDR < 0.05, 1.5-FC) upon TBBPA exposure. Functional and pathway analysis of the TBBPA transcriptional profile identified biological processes involved in nerve development, muscle filament sliding and contraction, and extracellular matrix disassembly and organization changed significantly. In addition, TBBPA also led to an elevation in the expression of genes encoding uridine diphosphate glucuronyl transferases (ugt), which could affect thyroxine (T4) metabolism and subsequently lead to neurobehavioral changes. In summary, TBBPA exposure during a narrow, sensitive developmental window perturbs various molecular pathways and results in neurobehavioral deficits in zebrafish. (C) 2016 Published by Elsevier Ltd.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21307096]; National Environmental Protection Public Welfare Science and Technology Research Program of China [201309047]; US EPA STAR grantUnited States Environmental Protection Agency [R835796]
出版者	ELSEVIER SCI LTD
出版地	OXFORD
ISSN	0269-7491
EISSN	1873-6424
卷号	216 页码:53-63
DOI	10.1016/j.envpol.2016.05.059
页数	56
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
WOS记录号	WOS:000383930500007
收录类别	SCIE ; PUBMED ; EI ; SCOPUS
EI入藏号	20162202450053
EI主题词	Flame retardants
URL	查看原文
PubMed ID	27239688
SCOPUSEID	2-s2.0-84970966305
通讯作者地址	[Lin, Kuangfei]State Environ. Protect. Key Lab. of Environmental Risk Assessment and Control on Chemical Process,School of Resources and Environmental Engineering,East China University of Science and Technology,No.130, Mei Long Road,Shanghai,200237,China
Scopus学科分类	Toxicology;Pollution;Health, Toxicology and Mutagenesis
TOP期刊	TOP期刊
引用统计	被引频次：56[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/7073
专题	公共卫生学院_健康与环境生态研究所公共卫生学院_环境安全与健康风险评估研究院
通讯作者	Lin, Kuangfei
作者单位	1.State Environ. Protect. Key Lab. of Environmental Risk Assessment and Control on Chemical Process,School of Resources and Environmental Engineering,East China University of Science and Technology,No.130, Mei Long Road,Shanghai,200237,China; 2.Institute of Environmental Safety and Human Health,Wenzhou Medical University,Wenzhou,325035,China; 3.Environmental and Molecular Toxicology,Sinnhuber Aquatic Research Laboratory,Environmental Health Sciences Center,Oregon State University,Corvallis,97333,United States
第一作者单位	温州医科大学
推荐引用方式 GB/T 7714	Chen, Jiangfei,Tanguay, Robert L.,Xiao, Yanyan,et al. TBBPA exposure during a sensitive developmental window produces neurobehavioral changes in larval zebrafish[J]. ENVIRONMENTAL POLLUTION,2016,216:53-63.
APA	Chen, Jiangfei., Tanguay, Robert L.., Xiao, Yanyan., Haggard, Derik E.., Ge, Xiaoqing., ... & Lin, Kuangfei. (2016). TBBPA exposure during a sensitive developmental window produces neurobehavioral changes in larval zebrafish. ENVIRONMENTAL POLLUTION, 216, 53-63.
MLA	Chen, Jiangfei,et al."TBBPA exposure during a sensitive developmental window produces neurobehavioral changes in larval zebrafish".ENVIRONMENTAL POLLUTION 216(2016):53-63.