鱼油干预对大鼠“术后疲劳”的影响

doi:10.3760/cma.j.issn.1674-635X.2012.03.005

科研成果详情

题名	鱼油干预对大鼠“术后疲劳”的影响
其他题名	Effect of perioperative use of fish oil on "post-operative fatigue" of rat
作者	王强 1; 杜璐迪 1; 陈必成 2; 周峰1; 闫竞一1; 余震1
发表日期	2012-06-30
发表期刊	中华临床营养杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	小动物“术后疲劳” 鱼油炎症开放式腹部手术行为学
其他关键词	Post-operative fatigue ; Fish oil ; Inflammatory ; Open abdominal surgery ; Behavior
摘要	目的探讨围手术期应用鱼油对大鼠"术后疲劳"的影响。方法 60只SD大鼠,经术前1周行为学训练,以其结果为依据筛选出表现较差的12只为血清空白对照组,余48只按随机数字表法随机均分为模型组和鱼油干预组。鱼油干预组于术前3 d至术后7 d腹腔注射鱼油[2 ml/(kg·d)],模型组同时段注射生理盐水[2 ml/(kg·d)],术后1、3、5、7 d,Morris水迷宫和悬尾实验行为学评估,检测外周血白介素(IL)-1β、IL-6、肿瘤坏死因子-α(TNF-α)和超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-PX)活力。结果血清指标:鱼油干预组术后1、3 d IL-6[(66.22±8.80)、(56.03±1.19)pg/ml]明显低于模型组[(83.30±10.69)、(82.72±24.27)pg/ml](P=0.034,P=0.038);术后1、3、5、7 d TNF-α[(104.36±5.02)、(84.49±7.81)、(64.47±2.89)、(39.29±2.52)pg/ml]明显低于模型组[(120.01±14.99)、(119.68±8.84)、(75.29±2.58)、(41.96±1.65)pg/ml](P=0.014,P=0.003,P=0.000,P=0.004);术后1、3、5、7 d IL-1β[(155.11±9.08)、(79.39±5.86)、(57.26±16.07)、(35.42±1.53)pg/ml]明显低于模型组[(204.87±30.61)、(198.82±54.83)、(152.12±29.06)、(64.35±2.70)pg/ml](P=0.024,P=0.002,P=0.000,P=0.000);术后5 d SOD(1.08±0.08)μmol/L明显高于模型组(0.71±0.06)μmol/L(P=0.000);术后5、7 d GSH-PX[(31.21±1.30)、(30.78±1.83)μmol/L]明显高于模型组[(25.03±1.74)、(27.57±3.57)μmol/L](P=0.000,P=0.036)。行为学:悬尾实验鱼油干预组术后1、3 d挣扎阈上面积[(6620±1390)、(7011±1472)mv·s]较模型组[(4739±1040)、(4344±1130)mv·s]明显增加(P=0.048,P=0.043);累积不动时间[(118.42±10.05)、(101.02±8.68)s]和单次最长静止时间[(55.39±7.70),(56.60±5.88)s]较模型组[(135.08±12.44)、(131.02±9.24)s;(65.73±3.78)、(64.93±3.25)s]明显减少(P=0.042,P=0.012,P=0.043,P=0.042)。Morris水迷宫鱼油干预组术后3、5 d逃避潜伏期[(48.263±1.815)、(44.955±2.567)s]较模型组[(51.543±1.990)、(49.956±2.888)s]明显减少(P=0.035,P=0.035);术后1、3、5、7 d穿越平台次数[(1.04±0.25)、(1.95±0.49)、(2.42±0.41)、(3.21±0.53)次]较模型组[(0.58±0.26)、(1.20±0.33)、(1.50±0.39)、(2.17±0.68)次]明显增加(P=0.002,P=0.003,P=0.018,P=0.035)。结论围手术期鱼油干预能有效改善大鼠术后的炎症反应,可能增强抗氧化防御能力,改善"术后疲劳"。
其他摘要	Objective To investigate the effect of perioperative use of fish oil on "post-operative fatigue"(POF) of rat.Methods After one week's preoperative behavior training,12 rats presented poor behavior were excluded from 60 healthy adult male SD rats as the normal controls of serum parameters.The remaining 48 rats were randomly divided into model group and fish oil treatment group by random number table.The fish oil treatment group received 10 days' (3 days before surgery and 7 days after surgery) intraperitoneal injection of fish oil [2 ml/( kg · d) ],and the model group with saline.On the 1st,3rd,5th,and 7th post-operative day,rats were assessed by Morris water-maze and tail suspension test.Serum levels of interleukin ( IL)-1β,IL-6,tumor necrosis factor-α (TNF-α),superoxide dismutase (SOD),and glutathione peroxidase (GSH-PX) were measured.Results Serum parameters:on the 1st and 3rd post-operative day,the IL-6 level in the fish oil treatment group [ (66.22 ±8.80),(56.03 ± 1.19) pg/ml] was significantly lower than in model group [ (83.30 ± 10.69),(82.72 ± 24.27) pg/ml ] (P =0.034,P =0.038 ) ; on the 1 st,3rd,5th,and 7th post-operative day,the TNF-α level in the fish oil treatment group [ ( 104.36 ±5.02),(84.49 ±7.81 ),(64.47 ±2.89),(39.29 ±2.52)pg/ml ] was significantly lower than in model group [ ( 120.01 ± 14.99 ),( 119.68 ± 8.84),(75.29 ± 2.58 ),(41.96±1.65) pg/ml] (P=0.014,P=0.003,P=0.000,P=0.004); onthe1st,3rd,5th,and 7th postoperative day,the IL-1β level [(155.11 ±9.08),(79.39±5.86),(57.26±16.07),(35.42±1.53) pg/ml]was significantly lower than model group [ (204.87±30.61), (198.82±54.83), (152.12±29.06),(64.35 ± 2.70) pg/ml ] ( P =0.024,P =0.002,P =0.000,P =0.000) ; on the 5th postoperative day,SOD ( 1.08±0.08) μmol/L was significantly higher than model group (0.71±0.06) μmoL/L (P=0.000) ; on the 5th and 7th postoperative day,GSH-PX [ (31.21 ± 1.30), (30.78 ± 1.83) μmol/L] was significantly higher than model group [ (25.03 ±1.74),(27.57±3.57) μ mol/L] (P=0.000,P=0.036).Behavior:in tail suspension test,on the 1st and 3rd postoperative day,value of struggle in fish oil treatment group [ (6620 ± 1390),(7011 ± 1472) mv · s] was significantly higher than in model group [ (4739 ± 1040),(4344 ± 1130) mv · s](P=0.048,P=0.043); cumulative fixed time [ (118.42±10.05), (101.02±8.68) s] and single rest time [ (55.39±7.70),(56.60±5.88) s] was lower thanin modelgroup [ (135.08+12.44),(131.02±9.24) s; (65.73±3.78),(64.93±3.25) s] (P=0.042,P=0.012,P=0.043,and P=0.042).In Morris water-maze,on the 3rd and 5th postoperative day,escape latent period of fish oil treatment group [ (48.263 ±1.815),(44.955±2.567) s] was lower than model group [ (51.543±1.990),(49.956±2.888) s] (P=0.035,P=0.035) ; on the 1st,3rd,5th,and 7th postoperative day,the cross platform number (1.04±0.25,1.95±0.49,2.42 ±0.41,3.21 ±0.53) was significantly higher than in model group (0.58 ±0.26,1.20±0.33,1.50±0.39,2.17±0.68) (P=0.002,P=0.003,P=0.018,P=0.035).Conclusion Perioperative use of fish oil can reduce postoperative inflammatory response,enhance antioxidant defense capability,and mitigate post-operative fatigue.
资助项目	国家自然科学基金（81070702）;浙江省重点扶植学科资助（07-F03）;浙江省医药卫生科技计划（2011ZDA018）
ISSN	1674-635X
卷号	20 期号:03 页码:153-157
DOI	10.3760/cma.j.issn.1674-635X.2012.03.005
页数	0
收录类别	CNKI ; 万方 ; ISTIC
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引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/67948
专题	附属第一医院附属第一医院_外科实验室
作者单位	1.温州医学院附属第一医院普外科; 2.温州医学院外科实验室
第一作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	王强,杜璐迪,陈必成,等. 鱼油干预对大鼠“术后疲劳”的影响[J]. 中华临床营养杂志,2012,20(03):153-157.
APA	王强, 杜璐迪, 陈必成, 周峰, 闫竞一, & 余震. (2012). 鱼油干预对大鼠“术后疲劳”的影响. 中华临床营养杂志, 20(03), 153-157.
MLA	王强,et al."鱼油干预对大鼠“术后疲劳”的影响".中华临床营养杂志 20.03(2012):153-157.