Serum connective tissue growth factor is a highly discriminatory biomarker for the diagnosis of rheumatoid arthritis

doi:10.1186/s13075-017-1463-1

科研成果详情

题名	Serum connective tissue growth factor is a highly discriminatory biomarker for the diagnosis of rheumatoid arthritis
作者	Yang, Xinyu1; Lin, Ke 2; Ni, Shanmin 2; Wang, Jianmin 3; Tian, Qingqing 2; Chen, Huaijun 2; Brown, Matthew A.4,5; Zheng, Kaidi2; Zhai, Weitao 6; Sun, Li7; Jin, Shengwei8; Wang, Jianguang2
发表日期	2017-11-22
发表期刊	ARTHRITIS RESEARCH & THERAPY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Rheumatoid arthritis CTGF ACPA Rheumatoid factor Biomarker
其他关键词	CITRULLINATED PEPTIDE ANTIBODIES ; CLASSIFICATION CRITERIA ; COMBINED TESTS ; ACCURACY ; METAANALYSIS ; DISORDERS
摘要	Background: Our previous proteomic study indicated that connective tissue growth factor (CTGF) may be a potential biomarker for rheumatoid arthritis (RA) diagnosis. The aim was to assess the performance of CTGF as a biomarker of RA. Method: Serum and synovial fluid CTGF was detected using a direct high sensitivity sandwich ELISA kit. Serum CTGF levels were tested for discriminatory capacity and optimal assay cutoffs determined in a training cohort of 98 cases of RA with 103 healthy controls. The assay performance was then validated in a further cohort of 572 patients (with RA (n = 217), ankylosing spondylitis (n = 92), gout (n = 74), osteoarthritis (n = 52), systemic lupus erythematosus (n = 72), or primary Sjgren's syndrome (pSS) (n = 65)). Results: Significant elevation of synovial fluid CTGF concentration was found in RA patients, demonstrating excellent diagnostic ability to predict RA (area under the curve (AUC) = 0.97). Similar results were found in serum CTGF detection. At the optimal cutoff value 88.66 pg/mL, the sensitivity, specificity, and the AUC was 0.86, 0.92, and 0.92, respectively, in the training cohort. Similar performance was observed in the validation cohort, with sensitivity, specificity, positive likelihood, and negative likelihood of 0.82, 0.91, 5.74, and 0.12, respectively. Stronger discriminatory capacity was seen with the combination of CTGF and anti-citrullinated protein antibody (ACPA) (AUC = 0.96) than with either ACPA or rheumatoid factor (RF) alone (AUC = 0.80 or 0.79, respectively). The discriminatory performance of serum CTGF was consistent across all inflammatory conditions tested (AUC > 0.92 in all cases), with the sole exception of pSS. Serum CTGF did not vary with symptom duration or disease activity. Conclusions: Serum CTGF is a promising diagnostic biomarker for RA, with performance in the current study better than either ACPA or RF.
资助项目	Public Technology Applied Research Foundation of Zhejiang Province of China [2016C33183]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81472055, 81672129]; National and Zhejiang College Students Innovative Entrepreneurial Training Program of China [201610343001, 2017R413063]; National Health and Medical Council (Australia) Senior Principal Fellowship; 1000 Talents Program fellowship from the Government of Zhejiang Province
出版者	BIOMED CENTRAL LTD
出版地	LONDON
ISSN	1478-6354
EISSN	1478-6362
卷号	19 期号:1 页码:257
DOI	10.1186/s13075-017-1463-1
页数	9
WOS类目	Rheumatology
WOS研究方向	Rheumatology
WOS记录号	WOS:000416172400004
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	29166915
PMC记录号	PMC5700625
Scopus记录号	2-s2.0-85034756965
通讯作者地址	[Sun, Li]The First Affiliated Hospital of Wenzhou Medical University,Department of Immunology and Rheumatology,Wenzhou,325035,China
scopus学科分类	Rheumatology;Immunology and Allergy;Immunology
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/6526
专题	药学院（分析测试中心）附属第二医院附属第一医院第二临床医学院、附属第二医院、育英儿童医院检验医学院（生命科学学院、生物学实验教学中心）_生物化学与分子生物学系第一临床医学院（信息与工程学院）、附属第一医院_精准医学中心实验室基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学免疫学
通讯作者	Sun, Li
作者单位	1.School of Pharmaceutical Sciences,Wenzhou Medical University,Department of Medicinal Chemistry,Wenzhou,China; 2.School of Basic Medical Sciences,Wenzhou Medical University,Department of Biochemistry,Wenzhou,325035,China; 3.Jiamusi Central Hospital,Department of Rheumatology,Jiamusi,China; 4.Queensland University of Technology,Princess Alexandra Hospital,Institute of Health and Biomedical Innovation,Translational Research Institute,Brisbane,Australia; 5.The First Affiliated Hospital of Wenzhou Medical University,Centre for Precision Medicine,Wenzhou,China; 6.Shanghai Guanghua Special Hospital for Rheumatoid Arthritis,Department of Orthopaedic Surgery,Shanghai,China; 7.The First Affiliated Hospital of Wenzhou Medical University,Department of Immunology and Rheumatology,Wenzhou,325035,China; 8.The Second Affiliated Hospital of Wenzhou Medical University,Department of Anesthesia and Critical Care,Wenzhou,325035,China
第一作者单位	药学院（分析测试中心）
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Yang, Xinyu,Lin, Ke,Ni, Shanmin,et al. Serum connective tissue growth factor is a highly discriminatory biomarker for the diagnosis of rheumatoid arthritis[J]. ARTHRITIS RESEARCH & THERAPY,2017,19(1):257.
APA	Yang, Xinyu., Lin, Ke., Ni, Shanmin., Wang, Jianmin., Tian, Qingqing., ... & Wang, Jianguang. (2017). Serum connective tissue growth factor is a highly discriminatory biomarker for the diagnosis of rheumatoid arthritis. ARTHRITIS RESEARCH & THERAPY, 19(1), 257.
MLA	Yang, Xinyu,et al."Serum connective tissue growth factor is a highly discriminatory biomarker for the diagnosis of rheumatoid arthritis".ARTHRITIS RESEARCH & THERAPY 19.1(2017):257.