姜黄素对TNF-α诱导大鼠星形胶质细胞MCP-1表达和释放的影响

doi:10.3760/cma.j.issn.0254-1416.2013.08.003

科研成果详情

题名	姜黄素对TNF-α诱导大鼠星形胶质细胞MCP-1表达和释放的影响
其他题名	Effect of curcumin on tumor necrosis factor-alpha-induced expression and release of monocyte chemoattractant protein-1 in rat astrocytes
作者	王睿娴 ; 袁超 ; 沈露露 ; 李军 ; 曹红
发表日期	2013-08-20
发表期刊	中华麻醉学杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	姜黄素星形细胞趋化因子CCL2 肿瘤坏死因子α
其他关键词	Curcumin ; Astrocytes ; Chemokine CCL2 ; Tumor necrosis factor-alpha
摘要	目的探讨姜黄素对TNF-α诱导大鼠星形胶质细胞单核细胞趋化蛋白-1(MCP-1)表达和释放的影响。方法新生SD大鼠5只,雌雄不拘,处死后取大脑皮层进行原代星形胶质细胞培养,采用神经胶质酸性蛋白免疫荧光染色法鉴定星形胶质细胞纯度。采用随机数字表法,将细胞分为6组(n=15):正常对照组(C组)不做任何处理;TNF-α组(T组):加入TNF-α,终浓度20 ng/ml,孵育2 h;TNF-α+不同浓度姜黄素组(Cur5组、Cur10组、Cur20组):加入姜黄素,终浓度分别为5、10、20μmol/L,孵育24 h,弃去培养液后再加入TNF-α,终浓度20 ng/ml孵育2 h;TNF-α+溶剂对照组(D组):加入DMSO,终浓度1μl/ml,孵育24 h后加入TNF-α,终浓度20 ng/ml孵育2 h。各组处理结束后,采用免疫组化法测定星形胶质细胞MCP-1的表达;采用ELISA方法检测MCP-1胶释放量。结果与C组相比,其余5组星形胶质细胞MCP-1表达和释放量升高(P<0.05);与T组相比,Cur20组MCP-1表达和释放量明显降低(P<0.05),Cur5组、Cur10组和D组星形胶质细胞MCP-1表达和释放量差异无统计学意义(P>0.05);与Cur5组相比,Cur20组星形胶质细胞MCP-1表达和释放量降低(P<0.05),Cur10组差异无统计学意义(P>0.05)。结论姜黄素可抑制TNF-α诱导的大鼠星形胶质细胞MCP-1表达和释放,该作用与剂量有关,该作用可能是其减轻神经病理性痛的机制之一。
其他摘要	Objective To investigate the effect of curcumin on tumor necrosis factor-alpha (TNF-α) -in-duced expression and release of monocyte chemoattractant protein-1 (MCP-1) in rat astrocytes. Methods The primary astrocytes were prepared from the cerebral cortex of 5 neonatal Sprague-Dawley rats and cultured. The cultured cells were identified by immunofluorescence staining with glial fibrillary acid protein. The cells were then divided into 6 groups(n=15 each): control group (group C), TNF-α group (group T), TNF-α+different concentrations of curcumin groups (Cur5, Cur10 and Cur20 groups), and TNF-α+solvent control group (D group). TNF-α with the final concentration of 20 ng/ml was added and the cells were incubated for 2 h in T group. In Cur5, Cur10 and Cur20 groups, curcumin with the final concentrations of 5, 10 and 20 μmol/L was added, respectively, the cells were incubated for 24 h and then the culture medium was abandoned, TNF-α with the final concentration of 20 ng/ml was added and the cells were then incubated for another 2 h. In group D, dimethyl sulfoxide with the final concentration of 1 μl/ml was added, the cells were then incubated for 24 h, then TNF-α with the final concentration of 20 ng/ml was added and the cells were incubated for another 2 h. After treatment in each group, the expression of MCP-1 was determined by immunohistochemistry and the release of MCP-1 was determined by ELISA. Results Compared with group C, the expression and release of MCP-1 was significantly increased in the other five groups (P<0.05) . Compared with group T, the expression and release of MCP-1 was significantly decreased in group Cur20 (P<0.05), and no significant changes in the expression and release of MCP-1 were found in Cur5, Cur10 and D groups (P>0.05). Compared with group Cur5, the expression and release of MCP-1 was significantly decreased in group Cur20 (P<0.05), and no significant change in the expression and release of MCP-1 was found in group Curl0 (P>0.05). Conclusion Curcumin can inhibit TNF-α-induced expression and release of MCP-1 in rat astrocytes and the effect is dose-related and may be one of the mechanisms of curcumin-induced reduction of neurophathic pain.
资助项目	国家自然科学基金（81073125）;浙江省自然科学基金（Y2090252）
ISSN	0254-1416
卷号	33 期号:08 页码:916-919
DOI	10.3760/cma.j.issn.0254-1416.2013.08.003
页数	0
收录类别	CNKI ; 万方 ; 北大核心 ; ISTIC ; CSCD ; PKU
学科领域	医药、卫生 ; 外科学
URL	查看原文
CSCD记录号	CSCD:4943715
万方分类号	R749.16(精神病学) ; R318.08(医用一般科学) ; R651.150.2(外科学各论)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/64880
专题	附属第二医院温州医科大学
作者单位	1.温州医学院附属第二医院麻醉科; 2.温州医学院疼痛医学研究
推荐引用方式 GB/T 7714	王睿娴,袁超,沈露露,等. 姜黄素对TNF-α诱导大鼠星形胶质细胞MCP-1表达和释放的影响[J]. 中华麻醉学杂志,2013,33(08):916-919.
APA	王睿娴, 袁超, 沈露露, 李军, & 曹红. (2013). 姜黄素对TNF-α诱导大鼠星形胶质细胞MCP-1表达和释放的影响. 中华麻醉学杂志, 33(08), 916-919.
MLA	王睿娴,et al."姜黄素对TNF-α诱导大鼠星形胶质细胞MCP-1表达和释放的影响".中华麻醉学杂志 33.08(2013):916-919.