题名 | Butyrate Enhances the Intestinal Barrier by Facilitating Tight Junction Assembly via Activation of AMP-Activated Protein Kinase in Caco-2 Cell Monolayers |
作者 | |
发表期刊 | JOURNAL OF NUTRITION 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
其他关键词 | CHAIN FATTY-ACIDS ; GASTROINTESTINAL MUCOSAL BARRIER ; PARENTERAL-NUTRITION ; PARACELLULAR PERMEABILITY ; GENE-EXPRESSION ; NORMAL RATS ; COLON ; EPITHELIUM ; MECHANISM ; UPSTREAM |
摘要 | Butyrate, one of the SCFA, promotes the development of the intestinal barrier. However, the molecular mechanisms underlying the butyrate regulation of the intestinal barrier are unknown. To test the hypothesis that the effect of butyrate on the intestinal barrier is mediated by the regulation of the assembly of tight junctions involving the activation of the AMP-activated protein kinase (AMPK), we determined the effect of butyrate on the intestinal barrier by measuring the transepithelial electrical resistance (TER) and inulin permeability in a Caco-2 cell monolayer model. We further used a calcium switch assay to study the assembly of epithelial tight junctions and determined the effect of butyrate on the assembly of epithelial tight junctions and AMPK activity, We demonstrated that the butyrate treatment increased AMPK activity and accelerated the assembly of tight junctions as shown by the reorganization of tight junction proteins, as well as the development of TER. AMPK activity was also upregulated by butyrate during calcium switch-induced tight junction assembly. Compound C, a specific AMPK inhibitor, inhibited the butyrate-induced activation of AMPK. The facilitating effect of butyrate on the increases in TER in standard culture media, as well as after calcium switch, was abolished by compound C. We conclude that butyrate enhances the intestinal barrier by regulating the assembly of tight junctions. This dynamic process is mediated by the activation of AMPK. These results suggest an intriguing link between SCFA and the intracellular energy sensor for the development of the intestinal barrier. J. Nutr. 139: 1619-1625, 2009. |
资助项目 | NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R03-HD060753] |
出版者 | OXFORD UNIV PRESS |
出版地 | OXFORD |
ISSN | 0022-3166 |
EISSN | 1541-6100 |
卷号 | 139期号:9页码:1619-1625 |
DOI | 10.3945/jn.109.104638 |
WOS类目 | Nutrition & Dietetics |
WOS研究方向 | Nutrition & Dietetics |
WOS记录号 | WOS:000269163300003 |
收录类别 | SCIE ; SCOPUS |
发表日期 | 2009-09 |
URL | 查看原文 |
Scopus记录号 | 2-s2.0-69549114504 |
ESI高被引论文 | 2020-01 ; 2020-03 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/6486 |
专题 | 第二临床医学院、附属第二医院、育英儿童医院 |
通讯作者 | Lin, Jing |
作者单位 | 1.Mt Sinai Sch Med, Dept Pediat, New York, NY 10029 USA; 2.Wenzhou Med Coll, Yuying Childrens Hosp, Dept Pediat Surg, Wenzhou 325027, Peoples R China; 3.Tongji Univ, Sch Med, Dept Med Genet, Shanghai 200092, Peoples R China |
推荐引用方式 GB/T 7714 | Peng, Luying,Li, Zhong-Rong,Green, Robert S.,et al. Butyrate Enhances the Intestinal Barrier by Facilitating Tight Junction Assembly via Activation of AMP-Activated Protein Kinase in Caco-2 Cell Monolayers[J]. JOURNAL OF NUTRITION,2009,139(9):1619-1625. |
APA | Peng, Luying, Li, Zhong-Rong, Green, Robert S., Holzman, Ian R., & Lin, Jing. (2009). Butyrate Enhances the Intestinal Barrier by Facilitating Tight Junction Assembly via Activation of AMP-Activated Protein Kinase in Caco-2 Cell Monolayers. JOURNAL OF NUTRITION, 139(9), 1619-1625. |
MLA | Peng, Luying,et al."Butyrate Enhances the Intestinal Barrier by Facilitating Tight Junction Assembly via Activation of AMP-Activated Protein Kinase in Caco-2 Cell Monolayers".JOURNAL OF NUTRITION 139.9(2009):1619-1625. |
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