Butyrate Enhances the Intestinal Barrier by Facilitating Tight Junction Assembly via Activation of AMP-Activated Protein Kinase in Caco-2 Cell Monolayers

doi:10.3945/jn.109.104638

科研成果详情

题名	Butyrate Enhances the Intestinal Barrier by Facilitating Tight Junction Assembly via Activation of AMP-Activated Protein Kinase in Caco-2 Cell Monolayers
作者	Peng, Luying 1,3; Li, Zhong-Rong2; Green, Robert S.1; Holzman, Ian R.1; Lin, Jing 1
发表日期	2009-09
发表期刊	JOURNAL OF NUTRITION 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	CHAIN FATTY-ACIDS ; GASTROINTESTINAL MUCOSAL BARRIER ; PARENTERAL-NUTRITION ; PARACELLULAR PERMEABILITY ; GENE-EXPRESSION ; NORMAL RATS ; COLON ; EPITHELIUM ; MECHANISM ; UPSTREAM
摘要	Butyrate, one of the SCFA, promotes the development of the intestinal barrier. However, the molecular mechanisms underlying the butyrate regulation of the intestinal barrier are unknown. To test the hypothesis that the effect of butyrate on the intestinal barrier is mediated by the regulation of the assembly of tight junctions involving the activation of the AMP-activated protein kinase (AMPK), we determined the effect of butyrate on the intestinal barrier by measuring the transepithelial electrical resistance (TER) and inulin permeability in a Caco-2 cell monolayer model. We further used a calcium switch assay to study the assembly of epithelial tight junctions and determined the effect of butyrate on the assembly of epithelial tight junctions and AMPK activity, We demonstrated that the butyrate treatment increased AMPK activity and accelerated the assembly of tight junctions as shown by the reorganization of tight junction proteins, as well as the development of TER. AMPK activity was also upregulated by butyrate during calcium switch-induced tight junction assembly. Compound C, a specific AMPK inhibitor, inhibited the butyrate-induced activation of AMPK. The facilitating effect of butyrate on the increases in TER in standard culture media, as well as after calcium switch, was abolished by compound C. We conclude that butyrate enhances the intestinal barrier by regulating the assembly of tight junctions. This dynamic process is mediated by the activation of AMPK. These results suggest an intriguing link between SCFA and the intracellular energy sensor for the development of the intestinal barrier. J. Nutr. 139: 1619-1625, 2009.
资助项目	NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R03-HD060753]
出版者	OXFORD UNIV PRESS
出版地	OXFORD
ISSN	0022-3166
EISSN	1541-6100
卷号	139 期号:9 页码:1619-1625
DOI	10.3945/jn.109.104638
页数	7
WOS类目	Nutrition & Dietetics
WOS研究方向	Nutrition & Dietetics
WOS记录号	WOS:000269163300003
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	19625695
SCOPUSEID	2-s2.0-69549114504
ESI高被引论文	2020-01 ; 2020-03
通讯作者地址	[Lin, Jing]Department of Pediatrics,Mount Sinai School of Medicine,United States
Scopus学科分类	Medicine (miscellaneous);Nutrition and Dietetics
引用统计	被引频次：929[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/6486
专题	第二临床医学院、附属第二医院、育英儿童医院
通讯作者	Lin, Jing
作者单位	1.Department of Pediatrics,Mount Sinai School of Medicine,United States; 2.Department of Pediatric Surgery,Yuying Children's Hospital,Wenzhou Medical College,China; 3.Department of Medical Genetics,Tongji University School of Medicine,China
推荐引用方式 GB/T 7714	Peng, Luying,Li, Zhong-Rong,Green, Robert S.,et al. Butyrate Enhances the Intestinal Barrier by Facilitating Tight Junction Assembly via Activation of AMP-Activated Protein Kinase in Caco-2 Cell Monolayers[J]. JOURNAL OF NUTRITION,2009,139(9):1619-1625.
APA	Peng, Luying, Li, Zhong-Rong, Green, Robert S., Holzman, Ian R., & Lin, Jing. (2009). Butyrate Enhances the Intestinal Barrier by Facilitating Tight Junction Assembly via Activation of AMP-Activated Protein Kinase in Caco-2 Cell Monolayers. JOURNAL OF NUTRITION, 139(9), 1619-1625.
MLA	Peng, Luying,et al."Butyrate Enhances the Intestinal Barrier by Facilitating Tight Junction Assembly via Activation of AMP-Activated Protein Kinase in Caco-2 Cell Monolayers".JOURNAL OF NUTRITION 139.9(2009):1619-1625.