An anticaries experimental study of SD rat immunized by recombinant plasmid pEGFP-N1-Srv+ encoding the V-region of surface protein of Streptococcus mutans

科研成果详情

题名	An anticaries experimental study of SD rat immunized by recombinant plasmid pEGFP-N1-Srv+ encoding the V-region of surface protein of Streptococcus mutans
作者	Ning-ning Yang; Kui-fang He; Yi-huai Pan; Hui-ning Wang; Hui Deng; Jian-feng Ma
发表日期	2012-12-01
发表期刊	Shanghai kou qiang yi xue = Shanghai journal of stomatology 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Purpose: An anticaries DNA vaccine pEGFP-N1-Srv+ was used to immunize rats by different immune pathways. The expression of recombinant plasmid in different tissues in vivo and the specific immune response and protection effects against dental caries were observed. Methods: 20 SD rats were divided into 4 groups, immunized with the recombinant plasmid pEGFP-N1-SrV+ by submandibular gland-target injection(TSG), and intramuscular injection,respectively; then the expression of recombinant plasmid in different tissues were detected by immunohistochemistry technique. 24 SD rats were divided into 4 groups, immunized with recombinant plasmid pEGFP-N1-SrV+ of 100 μg, then boosted once after two weeks, through the same routes as above;then indirect ELISA technique was used to detect the specific antibodies. Keyes caries scores were used to evaluate the anticaries effects. The data was analyzed by using SPSS17.0 software package. Results: (1)Recombinant plasmid was positively expressed in the muscle fibers and submandibular glands.(2)The specific salivary anti-SR IgA and serum IgG were detected, and the peak time of the antibodies level appeared 4 weeks after initial . At the 4th week, the levels of specific anti-SR antibodies were higher in the experimental group than that in the negative control group. The levels of salivary specific anti-SR IgA were significantly higher in TSG immunization group than that in the intramuscular injection group. (3)Keyes caries scores were not significantly different between the experimental groups and negative control groups. recombinant plasmid pEGFP-N1-Srv+ expressed in vivo and effectively increased specific salivary anti-SR IgA and serum IgG, and TSG immunization route significantly increased the specific salivary anti-SR IgA compared with the intramuscular immunization route;however, the recombinant plasmid pEGFP-N1-Srv+ alone can not protect the rats from dental caries. Conclusions: The recombinant plasmid pEGFP-N1-SrV+ expressed in vivo and effectively increased specific salivary anti-SR IgA and serum IgC, and TSG immunization route significantly increased the specific salivary anti-SR IgA compared with the intramuscular immunization route; however, the recombinant plasmid pEGFP-N1-Srv+ alone can not protect the rats from dental caries.
ISSN	1006-7248
卷号	21 期号:6 页码:637-42.
收录类别	PUBMED ; SCOPUS
URL	查看原文
PubMed ID	23364546
SCOPUSEID	2-s2.0-84936753133
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/63543
专题	第一临床医学院（信息与工程学院）、附属第一医院_口腔医学_口腔科
作者单位	Department of Oral Medicine, School of Stomatology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China. yningning@126.com
第一作者单位	第一临床医学院（信息与工程学院）、附属第一医院_口腔医学_口腔科
第一作者的第一单位	第一临床医学院（信息与工程学院）、附属第一医院_口腔医学_口腔科
推荐引用方式 GB/T 7714	Ning-ning Yang,Kui-fang He,Yi-huai Pan,et al. An anticaries experimental study of SD rat immunized by recombinant plasmid pEGFP-N1-Srv+ encoding the V-region of surface protein of Streptococcus mutans[J]. Shanghai kou qiang yi xue = Shanghai journal of stomatology,2012,21(6):637-42..
APA	Ning-ning Yang, Kui-fang He, Yi-huai Pan, Hui-ning Wang, Hui Deng, & Jian-feng Ma. (2012). An anticaries experimental study of SD rat immunized by recombinant plasmid pEGFP-N1-Srv+ encoding the V-region of surface protein of Streptococcus mutans. Shanghai kou qiang yi xue = Shanghai journal of stomatology, 21(6), 637-42..
MLA	Ning-ning Yang,et al."An anticaries experimental study of SD rat immunized by recombinant plasmid pEGFP-N1-Srv+ encoding the V-region of surface protein of Streptococcus mutans".Shanghai kou qiang yi xue = Shanghai journal of stomatology 21.6(2012):637-42..

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