Diphenhydramine interferes with MTT reduction assay

科研成果详情

题名	Diphenhydramine interferes with MTT reduction assay
作者	Yao Shen; Chen-Hui Zhang; Wei-Wei Hu; Zhong Chen
发表日期	2011-03-01
发表期刊	Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Objective: To determine the effects of organic amine diphenhydramine on the 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide dye (MTT) reduction assay. Methods: The primarily cultured cortical astrocytes were incubated with various concentrations of diphenhydramine for 24 h. To analyze the effects of diphenhydramine and other organic amines on the MTT assay, the data obtained from the MTT assay were compared with the results obtained from morphological observation and hoechst 33342 and propidium iodide (PI) nucleus double staining. Result: The MTT assay showed that diphenhydramine (10(-4)mol/L), pyrilamine (10 (-4)mol/L) and zolantidine (10 (-5)mol/L) caused a significant increase in MTT reduction in astrocytes. However there was no proliferation, apoptosis or necrosis detected by hoechst and PI nucleus double staining. Light microscopy revealed that exocytosis of formazan granules was inhibited by diphenhydramine. Conclusion: Diphenhydramine and other organic amines may enhance MTT reduction by suppression of MTT formazan exocytosis in astrocytes, which may affect the results of cell viability studies.
ISSN	1008-9292
卷号	40 期号:2 页码:163-8.
收录类别	PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	21488212
Scopus记录号	2-s2.0-79957946991
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/63470
专题	基础医学院（机能实验教学中心）_生物科学系
作者单位	School of Biology Science, Wenzhou Medical College, Wenzhou 325035, China.
第一作者单位	生物科学系
第一作者的第一单位	生物科学系
推荐引用方式 GB/T 7714	Yao Shen,Chen-Hui Zhang,Wei-Wei Hu,et al. Diphenhydramine interferes with MTT reduction assay[J]. Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences,2011,40(2):163-8..
APA	Yao Shen, Chen-Hui Zhang, Wei-Wei Hu, & Zhong Chen. (2011). Diphenhydramine interferes with MTT reduction assay. Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 40(2), 163-8..
MLA	Yao Shen,et al."Diphenhydramine interferes with MTT reduction assay".Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences 40.2(2011):163-8..

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