Inhibitory effect of matrine on proliferation of human ovary malignant teratoma cell line PA-1 in vitro

科研成果详情

题名	Inhibitory effect of matrine on proliferation of human ovary malignant teratoma cell line PA-1 in vitro
作者	Cong-de Chen; Xiang Li; Hao-chuan Zhang
发表日期	2010-07-01
发表期刊	Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine 影响因子和分区
语种	英语
原始文献类型	Journal Article ; Research Support, Non-U.S. Gov't
摘要	Objective: To study the effect of matrine (MAT) on the proliferation of human ovary malignant teratoma cell line PA-1 in vitro. Methods: PA-1 cells allocated in different groups were treated with different concentrations (0.25 mg/mL, 0.5 mg/mL and 1.0 mg/mL) of MAT. The inhibitory effect of MAT and its dose- and time-effect relationship were detected with MTT; the apoptosis rate and cell cycle were evaluated by flow cytometry; and the changes of bcl-2/bax mRNA expression in cells were measured using semi-quantitative RT-PCR. Results: After being exposed to MAT, the PA-1 cell proliferation was decreased in a concentration- and time-dependent manner; cell apoptosis rate raised as the increasing concentration of MAT and acting time; cells retarded at G1 phase in the cell cycle dose-dependently; and the bcl-2/bax mRNA expression in cells dawn-regulated significantly. Conclusion: MAT can dose- and time-dependently inhibit the proliferation of PA-1 cell by reducing bcl-2/bax mRNA ratio to produce a G1 phase arresting in cell cycle.
ISSN	1003-5370
卷号	30 期号:7 页码:723-5.
收录类别	PUBMED
URL	查看原文
PubMed ID	20929131
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/63341
专题	第二临床医学院、附属第二医院、育英儿童医院_儿科学_儿童外科
作者单位	Department of Pediatric Surgery, Yuying Children Hospital of Wenzhou Medical College, Zhejiang. cxm@wzmc.net
第一作者单位	儿童外科
第一作者的第一单位	儿童外科
推荐引用方式 GB/T 7714	Cong-de Chen,Xiang Li,Hao-chuan Zhang. Inhibitory effect of matrine on proliferation of human ovary malignant teratoma cell line PA-1 in vitro[J]. Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine,2010,30(7):723-5..
APA	Cong-de Chen, Xiang Li, & Hao-chuan Zhang. (2010). Inhibitory effect of matrine on proliferation of human ovary malignant teratoma cell line PA-1 in vitro. Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine, 30(7), 723-5..
MLA	Cong-de Chen,et al."Inhibitory effect of matrine on proliferation of human ovary malignant teratoma cell line PA-1 in vitro".Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine 30.7(2010):723-5..

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