Studies on the establishment of a method to detect HPV 6b E7-specific antibodies of serum and cervical secretion from patients with condyloma acuminatum and its epidemiological significance

科研成果详情

题名	Studies on the establishment of a method to detect HPV 6b E7-specific antibodies of serum and cervical secretion from patients with condyloma acuminatum and its epidemiological significance
作者	Xiao-zhi Zhong; Shao Chen; Shan-li Zhu; Xiang-yang Xue; Wen-shu Li; Le-dan Wang; Li-fang Zhang
发表日期	2010-02-01
发表期刊	Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Objective: To establish a method for detection of the human papi11omavirus (HPV) 6b E7-specific antibodies in serum and cervical secretion from patients with condyloma acuminatum (CA). Methods: A full-length HPV 6b E7 gene was amplified by PCR from the CA tissue to construct the recombinant plasmid pET32a(+)/HPV 6b E7. The expression of prokaryotic protein was analyzed by SDS-PAGE and Western blot, then purified with Ni-NTA Agarose affinity column and used as an diagnostic antigen for establishing indirect ELISA method, to detect specific serum IgG and specific cervical secretion sIgA from 56 CA patients, 81 healthy control. Sera from 43 cervical cancer was served as control. HPV 6b DNA from 56 CA patients was identified by PCR. Results: Data showed that the nucleotide homology of cloned sequence was 99.5%, compared to the standard sequences of HPV 6b E7 gene (GenBank accession number: NC001355). A high level expression of E7 fusion protein was obtained in prokaryotic expression system (40 µg/ml). Based on HPV 6b E7 fusion protein being used as coating antigen, results from ELISA showed that the absorbance rates (A) of serum IgG from CA, cervix cancer and healthy control groups were 1.82 ± 0.48, 1.36 ± 0.39 and 1.39 ± 0.27, respectively. The level of IgG antibody in the serum of CA group was significantly higher than that in cervix cancer group and healthy control (P < 0.05). The A values of cervical secretion sIgA in CA and healthy control groups were 0.63 ± 0.26 and 0.53 ± 0.06, respectively, while the level of sIgA antibody in the cervical secretion of CA group was also significantly higher than that in healthy controls (P < 0.05). The positive rate of HPV 6b E7 DNA in CA tissue was 78.6% (44/56) by PCR method. When compared the results measured by PCR, the HPV 6b E7-specific IgG and sIgA antibodies by ELISA used to detect the patients infected with HPV 6b infection, showed that the sensitivity rates were 68.2% (30/44) and 54.6% (24/44) respectively, and the specificity were all 100% (12/12). Conclusion: Based on the serum and cervical secretion specific HPV 6b E7 antibodies from patients with CA to diagnose HPV 6b infection, results showed medium sensitivity and high specificity, and could further be used to investigate the epidemiological characteristics of HPV 6b infection.
ISSN	0254-6450
卷号	31 期号:2 页码:199-203.
收录类别	PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	21215084
Scopus记录号	2-s2.0-84875111936
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/63323
专题	检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系
作者单位	Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Wenzhou Medical College, Wenzhou 325035, China.
第一作者单位	病原生物学与免疫学系; 病原生物学与免疫学系
第一作者的第一单位	病原生物学与免疫学系
推荐引用方式 GB/T 7714	Xiao-zhi Zhong,Shao Chen,Shan-li Zhu,et al. Studies on the establishment of a method to detect HPV 6b E7-specific antibodies of serum and cervical secretion from patients with condyloma acuminatum and its epidemiological significance[J]. Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi,2010,31(2):199-203..
APA	Xiao-zhi Zhong., Shao Chen., Shan-li Zhu., Xiang-yang Xue., Wen-shu Li., ... & Li-fang Zhang. (2010). Studies on the establishment of a method to detect HPV 6b E7-specific antibodies of serum and cervical secretion from patients with condyloma acuminatum and its epidemiological significance. Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi, 31(2), 199-203..
MLA	Xiao-zhi Zhong,et al."Studies on the establishment of a method to detect HPV 6b E7-specific antibodies of serum and cervical secretion from patients with condyloma acuminatum and its epidemiological significance".Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 31.2(2010):199-203..