Preparation and identification of immune serum against recombinant fusion protein of rhoptry 2 and major surface protein 1 from Toxoplasma gondii

科研成果详情

题名	Preparation and identification of immune serum against recombinant fusion protein of rhoptry 2 and major surface protein 1 from Toxoplasma gondii
作者	Wen-shu Li; Shan-li Zhu; Peng-fei Wang; Li-fang Zhang; Tai-shan Min; Wei-da Huang
发表日期	2007-10-01
发表期刊	Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Objective: To prepare and identify immune serum against the recombinant fusion protein of rhoptry 2 (ROP2) and major surface protein 1(P30) from Toxoplasma gondii. Methods: The constructed recombinant plasmid of pET28b/ROP2-P30 was transformed to a bacterium BL21-Codon Plus (DE3)-RIL strain and was expressed under IPTG induction. Cells were lysed by multiple rounds of sonication to obtain supernatant and inclusion body respectively. The inclusion body was washed with 2 mol/L and 4 mol/L urea to remove the nonspecific protein. The washed products dissolved in 8 mol/L urea were received by SDS-PAGE. Two rabbits were immunized with the fusion protein rROP2-P30 and sera from the rabbits were collected. Immune diffusion test, indirect ELISA and Western-blot were used to detect antibody titer and specificity of the immune serum against rROP2-P30. Results: Immune diffusion test demonstrated that specific immune serum were obtained. Indirect ELISA confirmed that the antibody titer in the serum reached 1:12 800 and the rROP2-P30 was recognized by specific IgG in this serum by Western-blot analysis. Conclusion: Specific immune serum against the recombinant fusion protein rROP2-P30 has been prepared.
ISSN	1000-7423
卷号	25 期号:5 页码:397-400.
收录类别	PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	18441993
Scopus记录号	2-s2.0-58149234317
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/63086
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系
作者单位	Department of Microbiology and Immunology of Wenzhou Medical College, Wenzhou 325032, China.
第一作者单位	病原生物学与免疫学系
第一作者的第一单位	病原生物学与免疫学系
推荐引用方式 GB/T 7714	Wen-shu Li,Shan-li Zhu,Peng-fei Wang,et al. Preparation and identification of immune serum against recombinant fusion protein of rhoptry 2 and major surface protein 1 from Toxoplasma gondii[J]. Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases,2007,25(5):397-400..
APA	Wen-shu Li, Shan-li Zhu, Peng-fei Wang, Li-fang Zhang, Tai-shan Min, & Wei-da Huang. (2007). Preparation and identification of immune serum against recombinant fusion protein of rhoptry 2 and major surface protein 1 from Toxoplasma gondii. Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 25(5), 397-400..
MLA	Wen-shu Li,et al."Preparation and identification of immune serum against recombinant fusion protein of rhoptry 2 and major surface protein 1 from Toxoplasma gondii".Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 25.5(2007):397-400..

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