Mechanism of the formation of a special inv(Y): a case study

科研成果详情

题名	Mechanism of the formation of a special inv(Y): a case study
作者	Bo Zhou; Yan-Ping Tang; Yong-Zhang Liu
发表日期	2006-02-01
发表期刊	Yi chuan = Hereditas 影响因子和分区
语种	英语
原始文献类型	Article
摘要	We report for the first time in China, the characterization of a special abnormal inv(Y) with dual-color fluorescence in situ hybridization (D-FISH). We also study the mechanism of the formation of chromosome Y inversion and its relationship with the phenotype of recurrent spontaneous abortion. Using biotin-11-dUTP-labeled Yp11.3 breakpoint probe (No. 889) and CY3-labeled Yq12 heterochromatic region DNA probe (PY3.4), we performed D-FISH on a balanced translocation carrier with a [46, XY(90%)/46, X, inv(Y)(p11.3; q12)] karyotype determined by G-banding karyotyping, whose wife had recurrent miscarriages. The result of D-FISH shows that the percentage of cells with abnormal karyotypes is about 22%, higher than that determined by G-banding analysis. Moreover, besides the type of inversion diagnosed by G-banding, there are the two other abnormal karyotypes, including a type of chromatid inversion, which is difficult to be detected by conventional G-banding technique. The present of the three types of inversion confirms that the breakpoints of inv(Y) are heterogeneous. D-FISH is a powerful tool for the detection of chromosomal inversion due to its sensitivity and specificity.
ISSN	0253-9772
卷号	28 期号:2 页码:148-52.
收录类别	PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	16520308
Scopus记录号	2-s2.0-60549095999
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/62966
专题	基础医学院（机能实验教学中心）_生物科学系
作者单位	Department of Biology, College of Life Sciences, Wenzhou Medical College, Wenzhou, Zhejiang 325035, China. cabina@126.com
第一作者单位	生物科学系; 检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	生物科学系
推荐引用方式 GB/T 7714	Bo Zhou,Yan-Ping Tang,Yong-Zhang Liu. Mechanism of the formation of a special inv(Y): a case study[J]. Yi chuan = Hereditas,2006,28(2):148-52..
APA	Bo Zhou, Yan-Ping Tang, & Yong-Zhang Liu. (2006). Mechanism of the formation of a special inv(Y): a case study. Yi chuan = Hereditas, 28(2), 148-52..
MLA	Bo Zhou,et al."Mechanism of the formation of a special inv(Y): a case study".Yi chuan = Hereditas 28.2(2006):148-52..