Eukaryotic expression and tumor-targeting modification of human mutated-IL-18 fusion gene

科研成果详情

题名	Eukaryotic expression and tumor-targeting modification of human mutated-IL-18 fusion gene
作者	Jian-Xin Lü; Ying Peng; Zhe-Feng Meng
发表日期	2005-07-01
发表期刊	Yi chuan = Hereditas 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Interleukin-18 (IL-18) is a proinflammatory cytokinin. This protein has a role in regulating immune responses and exhibits significant anti- tumor activities. Epidermal growth factor (EGF) is an important growth factor that plays a central role in the regulation of cell cycle and differentiation. It was proposed that a targeted delivery of IL-18 by generation of IL-18-EGF fusion protein might decrease adverse effects and result in enhancing cytotoxic and antitumor activities. In the present study, a fusion protein, consisting of EGFR binding domain fused to human IL18 mature peptide via a linker peptide of (Gly4ser) 3, was constructed and expressed in the insect cell line Sf9 using Bac-to-Bac baculovirus expression system. We showed that the purified recombinant fusion protein induced similar levels of IFN-gamma to that of native IL-18 protein in human PBMC in the presence of ConA. Furthermore, EGF Receptor competitive test in human epithelial cancer A431 cell line showed that EGF- IL18 fusion protein can specifically bind with EGFR by competing with native EGF protein. The results suggested that EGF-IL18 fusion protein could specifically be targeted on tumor cells. The work may provide a new insight into the cytokinin for the tumor treatment.
ISSN	0253-9772
卷号	27 期号:4 页码:557-60.
收录类别	PUBMED ; SCOPUS
URL	查看原文
PubMed ID	16120577
SCOPUSEID	2-s2.0-55249113265
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/62919
专题	检验医学院（生命科学学院、生物学实验教学中心）_细胞与分子生物医学研究所
作者单位	Institute of Cellular and Molecular Medicine, Wenzhou Medical College, Wenzhou 325035, China. jx@wz.zj.cn
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）_细胞与分子生物医学研究所
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）_细胞与分子生物医学研究所
推荐引用方式 GB/T 7714	Jian-Xin Lü,Ying Peng,Zhe-Feng Meng. Eukaryotic expression and tumor-targeting modification of human mutated-IL-18 fusion gene[J]. Yi chuan = Hereditas,2005,27(4):557-60..
APA	Jian-Xin Lü, Ying Peng, & Zhe-Feng Meng. (2005). Eukaryotic expression and tumor-targeting modification of human mutated-IL-18 fusion gene. Yi chuan = Hereditas, 27(4), 557-60..
MLA	Jian-Xin Lü,et al."Eukaryotic expression and tumor-targeting modification of human mutated-IL-18 fusion gene".Yi chuan = Hereditas 27.4(2005):557-60..