The effect of acetylcholine and atropine on proliferation and differentiation and mAchR1 expression of human SK-N-SH cells

科研成果详情

题名	The effect of acetylcholine and atropine on proliferation and differentiation and mAchR1 expression of human SK-N-SH cells
作者	Xin Hu; Jian Xin Lu
发表日期	2005-08-01
发表期刊	Shi yan sheng wu xue bao 影响因子和分区
语种	英语
原始文献类型	Article
摘要	The SK-N-SH human neuroblastoma cells were treated with acetylcholine (Ach) and atropine (Atro), the proliferation activity of cells was detected by CCK-8 method and the cell cycle distribution was determined by flow cytometry. The changes of the mRNA and protein of mAchR1 and c-fos were analysed by fluorescence quantitative PCR, western blot and flow cytometry indirect-immunofluorescence. Results showed 1 mmol/L Ach could significantly stimulate the proliferation of SK-N-SH cells and 1 mmol/L Atro could prevent SK-N-SH cells from S phase growing into G2/M phase; 1 mmol/L Ach and 1 mmol/L Atro had feedback adjustment on the protein level of mAchR1, but had no significant effect on mRNA level of mAchR1. 1 mmol/L Ach increased the both levels of mRNA and protein of Fos obviously, this effect was reversed by Atro. All phenomena clue us cholinergic receptors joining the events of cell proliferation.
ISSN	0001-5334
卷号	38 期号:4 页码:287-96.
收录类别	PUBMED ; SCOPUS
URL	查看原文
PubMed ID	16231694
SCOPUSEID	2-s2.0-34648868771
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/62905
专题	检验医学院（生命科学学院、生物学实验教学中心）_细胞与分子生物医学研究所
作者单位	Institute of Cell and Molecular Biology, Wenzhou Medical College, Wenzhou.
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）_细胞与分子生物医学研究所
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）_细胞与分子生物医学研究所
推荐引用方式 GB/T 7714	Xin Hu,Jian Xin Lu. The effect of acetylcholine and atropine on proliferation and differentiation and mAchR1 expression of human SK-N-SH cells[J]. Shi yan sheng wu xue bao,2005,38(4):287-96..
APA	Xin Hu, & Jian Xin Lu. (2005). The effect of acetylcholine and atropine on proliferation and differentiation and mAchR1 expression of human SK-N-SH cells. Shi yan sheng wu xue bao, 38(4), 287-96..
MLA	Xin Hu,et al."The effect of acetylcholine and atropine on proliferation and differentiation and mAchR1 expression of human SK-N-SH cells".Shi yan sheng wu xue bao 38.4(2005):287-96..

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