Study on soluble guanylate cyclase in pulmonary hypertension rat model

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题名	Study on soluble guanylate cyclase in pulmonary hypertension rat model
作者	Xiao-dong Xia; Zheng-jie Xu; Yun-tian Bi; Yuan-rong Dai; Shu-zhen Wu
发表日期	2003-09-01
发表期刊	Zhonghua nei ke za zhi 影响因子和分区
语种	英语
原始文献类型	Journal Article ; Research Support, Non-U.S. Gov't
摘要	Objective: To investigate the mRNA and protein expressions of soluble guanylate cyclase (sGC) and its enzyme activity in pulmonary hypertension rat model which was reproduced by hypoxia and hypercapnia. Methods: Male Sprague-Dawley rats were randomly divided into hypoxic and hypercapnic group (HH group) and control group (C group). The protein expressions of sGC alpha(1) and sGC beta(1) subunits in medial and small pulmonary arteries was measured by immunohistochemistry method with a polycolonal antibody. The mRNA expression of sGC alpha(1) subunit of lung tissue was detected by in situ hybridization using sGC oligonuclear probe. Basal sGC enzyme activity and sodium nitroprusside (SNP)-stimulated sGC activity in lung homogenates were assayed with enzyme kinetic analysis. Results: The mean pulmonary artery pressure (mPAP), the ratio of right ventricle/left ventricle + septum [RV/(LV + S)] and the ratio of right ventricle/body weight (RV/BW) were significantly higher in HH group than those in C group. The protein expressions of sGC alpha(1) and sGC beta(1) subunits and mRNA expressions of sGC alpha(1) subunit were significantly decreased in the small and medium pulmonary arteries in HH group as compared with those in C group (P < 0.01). Basal sGC enzyme activity in HH group (32.03 +/- 7.17 pmol cGMP synthesized.mg protein(-1).min(-1)) was significantly lower than that in C group (114.76 +/- 18.37 pmol cGMP synthesized.mg protein(-1).min(-1), P < 0.01). The SNP significantly increased the sGC enzyme activity but the SNP-stimulated sGC enzyme activity of lung homogenates in HH group was significantly lower than that in C group (P < 0.01). Conclusions: The mRNA and protein expressions of sGC subunits and their enzyme activities in lung tissue of pulmonary hypertension rat model were reduced.
ISSN	0578-1426
卷号	42 期号:9 页码:628-31.
收录类别	PUBMED
URL	查看原文
PubMed ID	14514391
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/62870
专题	附属第二医院_呼吸内科
作者单位	Department of Respiratory Medicine, The Second Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang 325027, China.
第一作者单位	附属第二医院_呼吸内科
第一作者的第一单位	附属第二医院_呼吸内科
推荐引用方式 GB/T 7714	Xiao-dong Xia,Zheng-jie Xu,Yun-tian Bi,et al. Study on soluble guanylate cyclase in pulmonary hypertension rat model[J]. Zhonghua nei ke za zhi,2003,42(9):628-31..
APA	Xiao-dong Xia, Zheng-jie Xu, Yun-tian Bi, Yuan-rong Dai, & Shu-zhen Wu. (2003). Study on soluble guanylate cyclase in pulmonary hypertension rat model. Zhonghua nei ke za zhi, 42(9), 628-31..
MLA	Xiao-dong Xia,et al."Study on soluble guanylate cyclase in pulmonary hypertension rat model".Zhonghua nei ke za zhi 42.9(2003):628-31..

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