Detection of Angiostrongylus cantonensis circulating antigen by monoclonal antibodies

科研成果详情

题名	Detection of Angiostrongylus cantonensis circulating antigen by monoclonal antibodies
作者	Shao-hui Liang; Hui-cong Huang; Chang-wang Pan; Feng Tan
发表日期	2005-11-16
发表期刊	Zhonghua yi xue za zhi 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Angiostrongylus cantonensis Antibodies, monoclonal Antigen Enzyme-linked immunosorbent assay
摘要	Objective: To prepare monoclonal antibodies (McAbs) against soluble antigens of adult worms of Angiostrongylus cantonensis (A. cantonensis) on the purpose to detect CAg of A. cantonensis. Methods: Female BALB/c mice were immunized with soluble antigens of adult worms of A. cantonensis and the spleen cells were fused with myeloma SP2/0 cells. The hybridoma cell strains were screened by enzyme-linked immunosorbent assay (ELISA) and Western blotting. Two McAbs (3F1 and 4H2) were applied to detect the CAg in the sera of rats and mice infected with A. cantonensis and angiostrongyliasis patients respectively by double antibody sandwich ELISA. Results: Three McAbs against A. cantonensis adult were obtained. Two McAbs (3F1, 4H2) were identified as IgG1 and one McAb (2A2) was identified as IgM. The titers of culture fluid and ascites was 1:25,600, 1:25,600, 1:12,800 and 1:80,000, 1:80,000, 1:40,000 respectively. Western blotting results showed three McAb could be used to identify 15,000 protein of adult worms of A. cantonensis. The detection rates of the CAg in the sera of infected rats and mice were 84.2% (48/57) and 87.2% (41/47) respectively. The detection rate of the CAg in the sera of angiostrongyliasis patients was 86.4% (19/22), and no cross reactions with sera from patients with schistosomiasis, cysticercosis cellulose, paragonimiasis and trichinellosis were observed. The CAg in the sera from mice examined at different periods after infection revealed positive 2 week after inoculation and the titer of CAg peaked 4 week after inoculation. Conclusion: A new method of sandwich ELISA with high sensitivity and specificity to detect the serum A. cantonensis CAg has been obtained, it could be applicable to the diagnosis, observation of curative effect and epidemiology of angiostrongyliasis.
ISSN	0376-2491
卷号	85 期号:43 页码:3057-61.
收录类别	PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	16324407
Scopus记录号	2-s2.0-31344463767
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/62745
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学寄生虫学
作者单位	Department of Parasitology, Wenzhou Medical College, Wenzhou 325035, China.
第一作者单位	医学寄生虫学
第一作者的第一单位	医学寄生虫学
推荐引用方式 GB/T 7714	Shao-hui Liang,Hui-cong Huang,Chang-wang Pan,et al. Detection of Angiostrongylus cantonensis circulating antigen by monoclonal antibodies[J]. Zhonghua yi xue za zhi,2005,85(43):3057-61..
APA	Shao-hui Liang, Hui-cong Huang, Chang-wang Pan, & Feng Tan. (2005). Detection of Angiostrongylus cantonensis circulating antigen by monoclonal antibodies. Zhonghua yi xue za zhi, 85(43), 3057-61..
MLA	Shao-hui Liang,et al."Detection of Angiostrongylus cantonensis circulating antigen by monoclonal antibodies".Zhonghua yi xue za zhi 85.43(2005):3057-61..

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