Preparation and preliminary application of monoclonal antibodies against adult worm of Angiostrongylus cantonensis

科研成果详情

题名	Preparation and preliminary application of monoclonal antibodies against adult worm of Angiostrongylus cantonensis
作者	Feng Tan; Chang-wang Pan; Shao-hui Liiang; Hui-cong Huang
发表日期	2005-08-30
发表期刊	Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Objective: To develop and identify monoclonal antibodies (McAbs) against adult worm of Angiostrongylus cantonensis and observe its applicability. Methods: BALB/c mice were immunized with soluble antigen of adult worms of A. cantonensis. The spleen cells of immunized mice were fused with myeloma cell, and the hybridoma secreting high titer of McAbs with high specificity was screened. By using the McAbs, serum of angiostrongyliasis patient and sera of the rats infected with A. cantonensis were detected by Western blotting and double antibody sandwich ELISA respectively. Results: Three McAbs were established (2A2, 3F1, 4H2), which all showed no cross reaction with antigens of Schistosoma japonicum, Paragonimus westermani, Cysticercus cellulosae and Trichinella spiralis. Western blotting analysis demonstrated that the three McAbs recognized a Mr 15,000 soluble antigen of adult worm of A. cantonensis and recognized the Mr 24,000 and Mr 15,000 circulating antigens from the serum of angiostrongyliasis patient. The double antibody sandwich ELISA detection showed a positive rate of 76.5%. Conclusion: Three hybridoma cell lines against adult worm of A. cantonensis have been established which secret high titer of McAbs with high specificity and seem promising in detecting the circulating antigen of the angiostrongyliasis patient.
ISSN	1000-7423
卷号	23 期号:4 页码:209-12.
收录类别	PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	16296607
Scopus记录号	2-s2.0-59649123522
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/62709
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学寄生虫学
作者单位	Department of Parasitology, Wenzhou Medical College, Wenzhou 325035, China.
第一作者单位	医学寄生虫学
第一作者的第一单位	医学寄生虫学
推荐引用方式 GB/T 7714	Feng Tan,Chang-wang Pan,Shao-hui Liiang,et al. Preparation and preliminary application of monoclonal antibodies against adult worm of Angiostrongylus cantonensis[J]. Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases,2005,23(4):209-12..
APA	Feng Tan, Chang-wang Pan, Shao-hui Liiang, & Hui-cong Huang. (2005). Preparation and preliminary application of monoclonal antibodies against adult worm of Angiostrongylus cantonensis. Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 23(4), 209-12..
MLA	Feng Tan,et al."Preparation and preliminary application of monoclonal antibodies against adult worm of Angiostrongylus cantonensis".Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 23.4(2005):209-12..

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