Preliminary study on isolation, purification and hydrolytic activity of cysteine proteinases in Entamoeba histolytica

科研成果详情

题名	Preliminary study on isolation, purification and hydrolytic activity of cysteine proteinases in Entamoeba histolytica
作者	Z Yan; S L Chen; S Z Mao
发表日期	2001
发表期刊	Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Objective: To explore the invading mechanism of amebae in lamina porpria and observe the interaction between the cysteine proteinase (CP) of Entamoeba histolytica and laminin. Methods: CP was identified by laminin-sepharose affinity chromatography, followed by isolation, purification and inhibitor experiment. The hydrolytic activity was measured by gelatin electrophoresis. Results: Purified CP of E. histolytica showed a strong affinity with laminin. The molecular weight of CP is 27 kDa. It can be inhibited by EC-64 and exhibited a protein hydrolytic activity. Conclusion: The specific affinity and hydrolytic activity of CP might play an important role in its invasion to the basement membrane of intestinal mucosa.
ISSN	1000-7423
卷号	19 期号:3 页码:163-5.
收录类别	PUBMED ; SCOPUS
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Pubmed记录号	12571943
Scopus记录号	2-s2.0-0142030595
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/62535
专题	基础医学院（机能实验教学中心）_生物科学系_生物化学与分子生物学
作者单位	Department of Biochemistry, Wenzhou Medical College, Wenzhou 325027.
第一作者单位	生物化学与分子生物学
第一作者的第一单位	生物化学与分子生物学
推荐引用方式 GB/T 7714	Z Yan,S L Chen,S Z Mao. Preliminary study on isolation, purification and hydrolytic activity of cysteine proteinases in Entamoeba histolytica[J]. Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases,2001,19(3):163-5..
APA	Z Yan, S L Chen, & S Z Mao. (2001). Preliminary study on isolation, purification and hydrolytic activity of cysteine proteinases in Entamoeba histolytica. Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 19(3), 163-5..
MLA	Z Yan,et al."Preliminary study on isolation, purification and hydrolytic activity of cysteine proteinases in Entamoeba histolytica".Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 19.3(2001):163-5..

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