Identification of curcumin-inhibited extracellular matrix receptors in non-small cell lung cancer A549 cells by RNA sequencing

doi:10.1177/1010428317705334

科研成果详情

题名	Identification of curcumin-inhibited extracellular matrix receptors in non-small cell lung cancer A549 cells by RNA sequencing
作者	Li, Huiping1,2; Wu, Hongjin 3; Zhang, Hongfang 3; Li, Ying 3; Li, Shuang 3; Hou, Qiang 3; Wu, Shixiu 3; Yang, Shuan-Ying 1
发表日期	2017-06-15
发表期刊	TUMOR BIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Curcumin RNA sequencing lung cancer cell migration extracellular matrix receptors
其他关键词	FOCAL ADHESION KINASE ; PI3K/AKT SIGNALING PATHWAY ; DOWN-REGULATION ; EXPRESSION ; SURVIVAL ; APOPTOSIS ; INVASION ; RESISTANCE ; MIGRATION ; PROLIFERATION
摘要	Curcumin is a potent anti-cancer drug in several types of human cancers. Despite of several preclinical and clinical studies of curcumin, the precise mechanism of curcumin in cancer prevention has remained unclear. In our study, we for the first time investigated whole transcriptome alteration in A549 non-small cell lung cancer (NSCLC) cell lines after treatment with curcumin using RNA sequencing. We found that lots of genes and signaling pathways were significantly altered after curcumin treatment in A549 cells. With bioinformatics approaches (gene ontology, Kyoto Encyclopedia of Genes and Genomes, and STRING), we found that those curcumin altered genes were not only the genes that induce cell death but also those extracellular matrix receptors and mitogen-activated protein kinase signaling pathway genes which regulate cell migration and proliferation. Among those significantly altered genes, eight genes (COL1A1, COL4A1, COL5A1, LAMA5, ITGA3, ITGA2B, DDIT3, and DUSP1) were further examined by quantitative reverse transcription polymerase chain reaction and western blot analysis in four non-small cell lung cancer cell lines. Both in cell lines and in mouse model, the extracellular matrix receptors including the integrin (ITGA3 and ITGA2B), collagen (COL5A1), and laminin (LAMA5) were significantly inhibited by curcumin at messenger RNA and protein levels. Functional studies confirmed that curcumin not only induced A549 cell death but also repressed cell proliferation and migration by regulating extracellular matrix receptors. Collectively, our study suggests that curcumin may be used as a promising drug candidate for intervening lung cancer in future studies.
资助项目	Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LZ15H220001, LY14H160005]; Zhejiang medical and Health Ministry Training Program [2015PYA009]; National Natural Science Foundation of China (CN)National Natural Science Foundation of China (NSFC) [81402529]
出版者	SAGE PUBLICATIONS LTD
出版地	LONDON
ISSN	1010-4283
EISSN	1423-0380
卷号	39 期号:6
DOI	10.1177/1010428317705334
页数	13
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000403618600001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	28618934
SCOPUSEID	2-s2.0-85025112495
通讯作者地址	[Yang, Shuan-Ying]Department of Respiratory Medicine,The Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an,710004,China
Scopus学科分类	Cancer Research
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/6183
专题	第一临床医学院（信息与工程学院）、附属第一医院_急诊医学附属第一医院
通讯作者	Yang, Shuan-Ying
作者单位	1.Department of Respiratory Medicine,The Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an,China; 2.Department of Emergency Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 3.Cancer Research Institute,Hangzhou Cancer Hospital,Hangzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院; 急诊医学
推荐引用方式 GB/T 7714	Li, Huiping,Wu, Hongjin,Zhang, Hongfang,et al. Identification of curcumin-inhibited extracellular matrix receptors in non-small cell lung cancer A549 cells by RNA sequencing[J]. TUMOR BIOLOGY,2017,39(6).
APA	Li, Huiping., Wu, Hongjin., Zhang, Hongfang., Li, Ying., Li, Shuang., ... & Yang, Shuan-Ying. (2017). Identification of curcumin-inhibited extracellular matrix receptors in non-small cell lung cancer A549 cells by RNA sequencing. TUMOR BIOLOGY, 39(6).
MLA	Li, Huiping,et al."Identification of curcumin-inhibited extracellular matrix receptors in non-small cell lung cancer A549 cells by RNA sequencing".TUMOR BIOLOGY 39.6(2017).