Thioredoxin reductase system mediates iron binding in IscA and iron delivery for the iron-sulfur cluster assembly in IscU

doi:10.1074/jbc.M504638200

科研成果详情

题名	Thioredoxin reductase system mediates iron binding in IscA and iron delivery for the iron-sulfur cluster assembly in IscU
作者	Ding, Huangen 1,3; Harrison, Karyn 1; Lu, Jianxin 2
发表日期	2005-08-26
发表期刊	JOURNAL OF BIOLOGICAL CHEMISTRY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Bacteria Catalysis Escherichia coli Genes Physiology Sulfur Biogenesis Cystein Eukaryots Thioredoxin
其他关键词	FE-S CLUSTER ; ESCHERICHIA-COLI ; AZOTOBACTER-VINELANDII ; CYSTEINE DESULFURASE ; TRANSCRIPTION FACTOR ; SACCHAROMYCES-CEREVISIAE ; CRYSTAL-STRUCTURE ; SCAFFOLD PROTEIN ; 2FE-2S CLUSTERS ; MARITIMA ISCU
摘要	IscA is a key member of the iron-sulfur cluster assembly machinery found in bacteria and eukaryotes. Previously, IscA was characterized as an alternative iron-sulfur cluster assembly scaffold, as purified IscA can host transient iron-sulfur clusters. However, recent studies indicated that IscA is an iron-binding protein that can provide iron for the iron-sulfur cluster assembly in a proposed scaffold IscU ( Ding H., Clark, R. J., and Ding, B. ( 2004) J. Biol. Chem. 279, 37499 - 37504). To further elucidate the roles of IscA in the biogenesis of iron-sulfur clusters, we reevaluate the iron binding activity of IscA under physiologically relevant conditions. The results indicate that in the presence of the thioredoxin reductase system, Escherichia coli IscA binds iron with an iron association constant of 2.0 x 10(19) M-1 in vitro. Whereas all three components (thioredoxin 1, thioredoxin reductase and NADPH) in the thioredoxin reductase system are essential for mediating the iron binding in IscA, only catalytic amounts of thioredoxin 1 and thioredoxin reductase are required. In contrast, IscU fails to bind iron in the presence of the thioredoxin reductase system, suggesting that the iron binding in IscA is specific. Nevertheless, the thioredoxin reductase system can promote the iron-sulfur cluster assembly in IscU in the presence of the iron-loaded IscA, cysteine desulfurase ( IscS), and L-cysteine, demonstrating a physiologically relevant system for the biogenesis of iron-sulfur clusters. The results provide additional evidence for the hypothesis that IscA is capable of recruiting intracellular free iron and delivering the iron for the iron-sulfur cluster assembly in IscU.
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
出版地	BETHESDA
ISSN	0021-9258
EISSN	1083-351X
卷号	280 期号:34 页码:30432-30437
DOI	10.1074/jbc.M504638200
页数	54
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
WOS记录号	WOS:000231362500056
收录类别	SCIE ; EI ; SCOPUS
EI入藏号	2005369345749
EI主题词	Enzymes
URL	查看原文
PubMed ID	15985427
SCOPUSEID	2-s2.0-24044536614
通讯作者地址	[Ding, Huangen]Dept. of Biological Sciences,LA State University,United States
Scopus学科分类	Biochemistry;Molecular Biology;Cell Biology
TOP期刊	TOP期刊
引用统计	被引频次：65[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/5613
专题	检验医学院（生命科学学院、生物学实验教学中心）_细胞与分子生物医学研究所
通讯作者	Ding, Huangen
作者单位	1.Department of Biological Sciences,Louisiana State University,United States; 2.Institute of Cellular and Molecular Medicine,Wenzhou Medical College,China; 3.Dept. of Biological Sciences,LA State University,United States
推荐引用方式 GB/T 7714	Ding, Huangen,Harrison, Karyn,Lu, Jianxin. Thioredoxin reductase system mediates iron binding in IscA and iron delivery for the iron-sulfur cluster assembly in IscU[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2005,280(34):30432-30437.
APA	Ding, Huangen, Harrison, Karyn, & Lu, Jianxin. (2005). Thioredoxin reductase system mediates iron binding in IscA and iron delivery for the iron-sulfur cluster assembly in IscU. JOURNAL OF BIOLOGICAL CHEMISTRY, 280(34), 30432-30437.
MLA	Ding, Huangen,et al."Thioredoxin reductase system mediates iron binding in IscA and iron delivery for the iron-sulfur cluster assembly in IscU".JOURNAL OF BIOLOGICAL CHEMISTRY 280.34(2005):30432-30437.