Synergistic effects of Lactobacillus rhamnosus culture supernatant and bone marrow mesenchymal stem cells on the development of alcoholic steatohepatitis in mice

科研成果详情

题名	Synergistic effects of Lactobacillus rhamnosus culture supernatant and bone marrow mesenchymal stem cells on the development of alcoholic steatohepatitis in mice
作者	Cai, Chao1; Chen, Da-Zhi 2; Ge, Li-Chao 1; Chen, Wen-Kai 1; Ye, Sha-Sha1; Ye, Wei-Wei 1,3; Tao, Ying 1; Wang, Rui 1; Li, Ji 1; Lin, Zhuo1; Wang, Xiao-Dong 1; Xu, Lan-Man1,4,5; Chen, Yong-Ping 1
发表日期	2019
发表期刊	American journal of translational research 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Gut microbiota alcoholic liver disease bone marrow mesenchymal stem cells mTOR natural killer-like B cells. natural killer-like B cells
其他关键词	AUTOIMMUNE HEPATITIS ; AUTOPHAGY ; MICROBIOTA ; APOPTOSIS ; PATHWAY
摘要	The gut microbiota has been shown to play an important role in chronic liver disease. It has been found that both Lactobacillus rhamnosus and its culture supernatant have the potential to mitigate alcoholic steatohepatitis. However, the exact mechanism is still not fully understood. Bone marrow mesenchymal stem cells have immunosuppressive effects with few side effects. The synergistic effect between Lactobacillus rhamnosus culture supernatant and bone marrow mesenchymal stem cells (BMMSCs) deserves further observation. In this study, a mouse model of chronic alcoholic hepatitis was established by eight weeks of Lieber-DeCarli liquid diet feeding; and LGG-s, BMMSCs or a combination of the two were used to explore a new therapeutic method for alcoholic liver disease and to study the mechanism. The results showed that the combined LGG-s and BMMSC treatment might have a synergistic effect and could improve the symptoms of alcoholic hepatitis by regulating inflammation, autophagy and lymphocyte subsets through the PI3k/NF-kB and PI3K/mTOR pathways. With the treatment, the autophagy rate accelerated, and alcohol-induced natural killer B (NKB) cell and follicular helper T (TFH) cell numbers decreased. These findings suggest that the development of alcoholic hepatitis may occur via PI3K/NF-kB and PI3K/mTOR pathway overactivation as well as through NKB and TFH cell imbalances. Moreover, LGG-s and BMMSCs can regulate these factors and alleviate the disease.
资助项目	National Natural Science Foundation of China[2017Z-X10202201];
出版者	E-CENTURY PUBLISHING CORP
出版地	MADISON
ISSN	1943-8141
卷号	11 期号:9 页码:5703-5715.
页数	13
WOS类目	Oncology ; Medicine, Research & Experimental
WOS研究方向	Oncology ; Research & Experimental Medicine
WOS记录号	WOS:000488232800032
收录类别	PUBMED ; SCOPUS ; SCIE
URL	查看原文
PubMed ID	31632541
PMC记录号	PMC6789285
SCOPUSEID	2-s2.0-85076973685
通讯作者地址	[Wang, Xiao-Dong]Department of Infectious Diseases,Wenzhou Medical University,Hepatology Institute of Wenzhou Medical University,Wenzhou Key Laboratory of Hepatology,Wenzhou,Zhejiang,325000,China
Scopus学科分类	Molecular Medicine;Clinical Biochemistry;Cancer Research
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/55925
专题	附属第一医院其他_附属义乌医院（义乌市中心医院）
通讯作者	Wang, Xiao-Dong
作者单位	1.Department of Infectious Diseases,Wenzhou Medical University,Hepatology Institute of Wenzhou Medical University,Wenzhou Key Laboratory of Hepatology,Wenzhou,Zhejiang,325000,China; 2.Department of Gastroenterology,First Hospital of Peking University,Beijing,China; 3.Affiliated Yiwu Central Hospital,Wenzhou Medical University,Wenzhou,China; 4.Department of Infectious Diseases and Liver Diseases,Ningbo Medical Center Lihuili Eastern Hospital,Ningbo,315040,China; 5.Department of Infectious Diseases and Liver Diseases,Taipei Medical University Ningbo Medical Center,Ningbo,315040,China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Cai, Chao,Chen, Da-Zhi,Ge, Li-Chao,et al. Synergistic effects of Lactobacillus rhamnosus culture supernatant and bone marrow mesenchymal stem cells on the development of alcoholic steatohepatitis in mice[J]. American journal of translational research,2019,11(9):5703-5715..
APA	Cai, Chao., Chen, Da-Zhi., Ge, Li-Chao., Chen, Wen-Kai., Ye, Sha-Sha., ... & Chen, Yong-Ping. (2019). Synergistic effects of Lactobacillus rhamnosus culture supernatant and bone marrow mesenchymal stem cells on the development of alcoholic steatohepatitis in mice. American journal of translational research, 11(9), 5703-5715..
MLA	Cai, Chao,et al."Synergistic effects of Lactobacillus rhamnosus culture supernatant and bone marrow mesenchymal stem cells on the development of alcoholic steatohepatitis in mice".American journal of translational research 11.9(2019):5703-5715..