题名 | Comprehensive analysis of long noncoding RNA and mRNA in five colorectal cancer tissues and five normal tissues |
作者 | |
发表日期 | 2020-02-14 |
发表期刊 | BIOSCIENCE REPORTS 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
关键词 | colorectal cancer functional analysis lncRNA profiling mRNA profiling target gene. |
其他关键词 | TRANSCRIPTOME ; ANNOTATION ; MECHANISMS ; EXPRESSION ; INSIGHTS |
摘要 | The present study investigated the role of abnormally expressed mRNA and long noncoding RNA (lncRNA) in the development of colorectal cancer (CRC). We used lncRNA sequencing to analyze the transcriptome (mRNA and lncRNA) of five pairs of CRC tissues and adjacent normal tissues. The total expression of mRNAs and lncRNAs in each sample was determined using the R package and the gene expression was calculated using normalized FPKM. The structural features and expression of all detected lncRNAs were compared with those of mRNAs. Differentially expressed mRNAs were selected to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. The functional analysis of differentially expressed lncRNAs was performed by analyzing the GO and KEGG enrichment of predicted cis-regulated target genes. A total of 18.2 x 10(8) reads were obtained by sequencing, in which the clean reads reached >= 94.67%, with a total of 245.2 G bases. The number of mRNAs and lncRNAs differentially expressed in CRC tissues and normal tissues were 113 and 6, respectively. Further predictive analysis of target genes of lncRNAs revealed that six lncRNA genes had potential cis-regulatory effects on 13 differentially expressed mRNA genes and co-expressed with 53 mRNAs. Up-regulated CTD-2256P15.4 and RP11-229P13.23 were the most important lncRNAs in these CRC tissues and involved in cell proliferation and pathway in cancer. In conclusion, our study provides evidence regarding the mRNA and lncRNA transcription in CRC tissues, as well as new insights into the lncRNAs and mRNAs involved in the development of CRC. |
资助项目 | Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LY16H030013]; Wenzhou Science and Technology Bureau [Y20150062] |
出版者 | PORTLAND PRESS LTD |
出版地 | LONDON |
ISSN | 0144-8463 |
EISSN | 1573-4935 |
卷号 | 40期号:2页码:BSR20191139. |
DOI | 10.1042/BSR20191139 |
页数 | 12 |
WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
WOS记录号 | WOS:000518887600001 |
收录类别 | SCIE ; PUBMED ; SCOPUS |
URL | 查看原文 |
Pubmed记录号 | 32016349 |
PMC记录号 | PMC7028436 |
Scopus记录号 | 2-s2.0-85081971321 |
通讯作者地址 | [Li, Guo-Xin]Department of General Surgery,Nanfang Hospital,Southern Medical University,Guangzhou, Guangdong Province,510515,China |
scopus学科分类 | Biophysics;Biochemistry;Molecular Biology;Cell Biology |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/5570 |
专题 | 检验医学院(生命科学学院、生物学实验教学中心) 附属第一医院 检验医学院(生命科学学院、生物学实验教学中心)_病原生物学与免疫学系 基础医学院(机能实验教学中心)_病原生物学与免疫学系 |
通讯作者 | Li, Guo-Xin |
作者单位 | 1.Department of General Surgery,Nanfang Hospital,Southern Medical University,Guangzhou, Guangdong Province,510515,China; 2.Department of Hernia and Abdominal Wall Surgery,First Affiliated Hospital,Wenzhou Medical University,Wenzhou, Zhejiang Province,325000,China; 3.Institute of Glycobiological Engineering,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou, Zhejiang Province,325035,China; 4.Key Laboratory of Laboratory Medicine,Ministry of Education of China,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou, Zhejiang Province,325035,China; 5.Department of Microbiology and Immunology,Institute of Molecular Virology and Immunology,Institute of Tropical Medicine,Wenzhou Medical University,Wenzhou, Zhejiang Province,325035,China |
第一作者单位 | 附属第一医院 |
推荐引用方式 GB/T 7714 | Zhou, Zhen-Xu,Chen, Xiao-Ming,Zhang, Yu-Qi,et al. Comprehensive analysis of long noncoding RNA and mRNA in five colorectal cancer tissues and five normal tissues[J]. BIOSCIENCE REPORTS,2020,40(2):BSR20191139.. |
APA | Zhou, Zhen-Xu, Chen, Xiao-Ming, Zhang, Yu-Qi, Peng, Liu, Xue, Xiang-Yang, & Li, Guo-Xin. (2020). Comprehensive analysis of long noncoding RNA and mRNA in five colorectal cancer tissues and five normal tissues. BIOSCIENCE REPORTS, 40(2), BSR20191139.. |
MLA | Zhou, Zhen-Xu,et al."Comprehensive analysis of long noncoding RNA and mRNA in five colorectal cancer tissues and five normal tissues".BIOSCIENCE REPORTS 40.2(2020):BSR20191139.. |
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