Comprehensive analysis of long noncoding RNA and mRNA in five colorectal cancer tissues and five normal tissues

doi:10.1042/BSR20191139

科研成果详情

题名	Comprehensive analysis of long noncoding RNA and mRNA in five colorectal cancer tissues and five normal tissues
作者	Zhou, Zhen-Xu1,2; Chen, Xiao-Ming3,4; Zhang, Yu-Qi 3,4; Peng, Liu 3,4; Xue, Xiang-Yang5; Li, Guo-Xin 1
发表日期	2020-02-14
发表期刊	BIOSCIENCE REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	colorectal cancer functional analysis lncRNA profiling mRNA profiling target gene.
其他关键词	TRANSCRIPTOME ; ANNOTATION ; MECHANISMS ; EXPRESSION ; INSIGHTS
摘要	The present study investigated the role of abnormally expressed mRNA and long noncoding RNA (lncRNA) in the development of colorectal cancer (CRC). We used lncRNA sequencing to analyze the transcriptome (mRNA and lncRNA) of five pairs of CRC tissues and adjacent normal tissues. The total expression of mRNAs and lncRNAs in each sample was determined using the R package and the gene expression was calculated using normalized FPKM. The structural features and expression of all detected lncRNAs were compared with those of mRNAs. Differentially expressed mRNAs were selected to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. The functional analysis of differentially expressed lncRNAs was performed by analyzing the GO and KEGG enrichment of predicted cis-regulated target genes. A total of 18.2 x 10(8) reads were obtained by sequencing, in which the clean reads reached >= 94.67%, with a total of 245.2 G bases. The number of mRNAs and lncRNAs differentially expressed in CRC tissues and normal tissues were 113 and 6, respectively. Further predictive analysis of target genes of lncRNAs revealed that six lncRNA genes had potential cis-regulatory effects on 13 differentially expressed mRNA genes and co-expressed with 53 mRNAs. Up-regulated CTD-2256P15.4 and RP11-229P13.23 were the most important lncRNAs in these CRC tissues and involved in cell proliferation and pathway in cancer. In conclusion, our study provides evidence regarding the mRNA and lncRNA transcription in CRC tissues, as well as new insights into the lncRNAs and mRNAs involved in the development of CRC.
资助项目	Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LY16H030013]; Wenzhou Science and Technology Bureau [Y20150062]
出版者	PORTLAND PRESS LTD
出版地	LONDON
ISSN	0144-8463
EISSN	1573-4935
卷号	40 期号:2 页码:BSR20191139.
DOI	10.1042/BSR20191139
页数	12
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000518887600001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	32016349
PMC记录号	PMC7028436
Scopus记录号	2-s2.0-85081971321
通讯作者地址	[Li, Guo-Xin]Department of General Surgery,Nanfang Hospital,Southern Medical University,Guangzhou, Guangdong Province,510515,China
scopus学科分类	Biophysics;Biochemistry;Molecular Biology;Cell Biology
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/5570
专题	检验医学院（生命科学学院、生物学实验教学中心）附属第一医院检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Li, Guo-Xin
作者单位	1.Department of General Surgery,Nanfang Hospital,Southern Medical University,Guangzhou, Guangdong Province,510515,China; 2.Department of Hernia and Abdominal Wall Surgery,First Affiliated Hospital,Wenzhou Medical University,Wenzhou, Zhejiang Province,325000,China; 3.Institute of Glycobiological Engineering,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou, Zhejiang Province,325035,China; 4.Key Laboratory of Laboratory Medicine,Ministry of Education of China,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou, Zhejiang Province,325035,China; 5.Department of Microbiology and Immunology,Institute of Molecular Virology and Immunology,Institute of Tropical Medicine,Wenzhou Medical University,Wenzhou, Zhejiang Province,325035,China
第一作者单位	附属第一医院
推荐引用方式 GB/T 7714	Zhou, Zhen-Xu,Chen, Xiao-Ming,Zhang, Yu-Qi,et al. Comprehensive analysis of long noncoding RNA and mRNA in five colorectal cancer tissues and five normal tissues[J]. BIOSCIENCE REPORTS,2020,40(2):BSR20191139..
APA	Zhou, Zhen-Xu, Chen, Xiao-Ming, Zhang, Yu-Qi, Peng, Liu, Xue, Xiang-Yang, & Li, Guo-Xin. (2020). Comprehensive analysis of long noncoding RNA and mRNA in five colorectal cancer tissues and five normal tissues. BIOSCIENCE REPORTS, 40(2), BSR20191139..
MLA	Zhou, Zhen-Xu,et al."Comprehensive analysis of long noncoding RNA and mRNA in five colorectal cancer tissues and five normal tissues".BIOSCIENCE REPORTS 40.2(2020):BSR20191139..