Evaluation of correlation between NF-κB mediated PAI-1 gene and sepsis

科研成果详情

题名	Evaluation of correlation between NF-κB mediated PAI-1 gene and sepsis
作者	L-X Wang; L-M Tang; G-Q Pan; C-X Yan; L Lu; Y-F Liang
发表日期	2017
发表期刊	European review for medical and pharmacological sciences 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Objective:The present study is aimed to explore the correlation between PAI-1 gene mediated by NF-κB signaling pathway and human sepsis. Patients and methods:In this study, we used 74 cases of sepsis patients preserved by the laboratory as the observation group, and 68 cases of healthy people served the control group. Further methods like fluorescence quantitative PCR, enzyme-linked immunosorbent assay, Western-blotting were used to determine NF-B expression, NF-κB gene mRNA analyses and protein expression on different research subjects. Further, the positive expression rates of PAI-1 gene in the observation group and the control group were determined by immunohistochemistry. Results:The expression levels of NF-κB and PAI-1 gene mRNA in the blood of the observation group significantly increased in comparison to control group (X2 = 3.24, p < 0.05; X2 = 2.81, p < 0.05). Also, NF-κF and PAI-1 gene protein expressions (0.14 ug/l, 0.32 ug/l) were significantly higher in the observation group in comparison to control group (p < 0.05). The results of blood glucose measurement showed that the fasting blood glucose (14.3 mmol/l) in the observation group was significantly higher than that in the control group (4.6 mmol/l). Immunohistochemical were also in sync with above results. Conclusions:The present study concludes that PAI-1 gene expression gets significantly increased via NF-κF signaling pathway during sepsis.
ISSN	1128-3602
EISSN	2284-0729
卷号	21 期号:1 页码:30-36.
收录类别	PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28121360
SCOPUSEID	2-s2.0-85026320677
Scopus学科分类	Pharmacology (medical)
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/55618
专题	第二临床医学院，附属第二医院、育英儿童医院_麻醉学_ICU
作者单位	Department of Pediatric ICU, The Second Affiliated Hospital and Yuying Children&s Hospital of Wenzhou Medical University, Wenzhou, P. R. China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院_麻醉学_ICU
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院_麻醉学_ICU
推荐引用方式 GB/T 7714	L-X Wang,L-M Tang,G-Q Pan,et al. Evaluation of correlation between NF-κB mediated PAI-1 gene and sepsis[J]. European review for medical and pharmacological sciences,2017,21(1):30-36..
APA	L-X Wang, L-M Tang, G-Q Pan, C-X Yan, L Lu, & Y-F Liang. (2017). Evaluation of correlation between NF-κB mediated PAI-1 gene and sepsis. European review for medical and pharmacological sciences, 21(1), 30-36..
MLA	L-X Wang,et al."Evaluation of correlation between NF-κB mediated PAI-1 gene and sepsis".European review for medical and pharmacological sciences 21.1(2017):30-36..