Application of citrate as a tricarboxylic acid (TCA) cycle intermediate, prevents diabetic-induced heart damages in mice

科研成果详情

题名	Application of citrate as a tricarboxylic acid (TCA) cycle intermediate, prevents diabetic-induced heart damages in mice
作者	Liang, Qianqian 1; Wang, Baoyu 1; Pang, Lingxia2; Wang, Youpei2; Zheng, Meiqin3; Wang, Qing 3; Yan, Jingbin 4; Xu, Jinzhong 5
发表日期	2016
发表期刊	Iranian journal of basic medical sciences 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Citrate Diabetes Heart Nitration Tricarboxylic acid. Tricarboxylic acid
其他关键词	CARDIAC PATHOLOGICAL-CHANGES ; OXIDATIVE STRESS ; METALLOTHIONEIN ; CARDIOMYOPATHY ; ATP ; COMPLICATIONS ; MITOCHONDRIA ; INHIBITION ; ACTIVATION ; NITRATION
摘要	Objectives:Higher cellular reactive oxygen species (ROS) levels is important in reducing cellular energy charge (EC) by increasing the levels of key metabolic protein, and nitrosative modifications, and have been shown to damage the cardiac tissue of diabetic mice. However, the relation between energy production and heart function is unclear. Materials and methods:Streptozotocin (STZ, 150 mg/kg body weight) was injected intraperitoneally once to mice that had been fasted overnight for induction of diabetes. After diabetic induction, mice received citrate (5 µg/kg) through intraperitoneal injection every other day for 5 weeks. The caspase-3, plasminogen activator inhibitor 1 (PAI1), protein kinase B (PKB), commonly known as AKT and phosphorylated-AKT (p-AKT) proteins were examined to elucidate inflammation and apoptosis in the heart. For histological analysis, heart samples were fixed with 10% formalin and stained with hematoxylin-eosin (HE) and Sirius red to assess pathological changes and fibrosis. The expression levels[AGA1] of marker proteins, tyrosine nitration, activity of ATP synthase and succinyl-CoA3-ketoacid coenzyme A transferase-1 (SCOT), and EC were measured. Results:Intraperitoneal injection of citrate significantly reduced caspase-3 and PAI-1 protein levels and increased p-AKT level on the 5(th) week; EC in the heart was found to be increased as well. Further, the expression level, activity, and tyrosine nitration of ATP synthase and SCOT were not affected after induction of diabetes. Conclusion:Results indicate that application of citrate, a tricarboxylic acid (TCA) cycle intermediate, might alleviate cardiac dysfunction by reducing cardiac inflammation, apoptosis, and increasing cardiac EC.
资助项目	Western Michigan University[CZ5342];
出版者	MASHHAD UNIV MED SCIENCES
出版地	MASHHAD
ISSN	2008-3866
EISSN	2008-3874
卷号	19 期号:1 页码:43-8.
页数	6
WOS类目	Medicine, Research & Experimental ; Pharmacology & Pharmacy
WOS研究方向	Research & Experimental Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:000371470600007
收录类别	PUBMED ; SCOPUS ; SCIE
URL	查看原文
Pubmed记录号	27096063
PMC记录号	PMC4823615
Scopus记录号	2-s2.0-84969437840
通讯作者地址	[Xu, Jinzhong]Wenling first people’s hospital,The Affiliated Hospital of Wenzhou Medical University,Wenling,317500,China
scopus学科分类	Biophysics;Biochemistry;Immunology and Microbiology (all);Drug Discovery;Clinical Biochemistry
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/55599
专题	基础医学院（机能实验教学中心）_基础医学实验教学中心_机能实验教学中心（挂靠、校级）眼视光学院（生物医学工程学院）、附属眼视光医院其他_附属温岭医院（温岭市第一人民医院）
通讯作者	Xu, Jinzhong
作者单位	1.Department of Emergency,Zhengzhou Central Hospital,Zhengzhou University,Zhengzhou,450007,China; 2.Function Experiment Teaching Center,Wenzhou Medical University,Wenzhou,325305,China; 3.The Affiliated Eye Hospital,Wenzhou Medical University,Wenzhou,325000,China; 4.Department of Pain management,Wenzhou Hospital of integrated Chinese and Western Medicine,Wenzhou,325000,China; 5.Wenling First People’s Hospital,The Affiliated Hospital of Wenzhou Medical University,Wenling,317500,China
通讯作者单位	温州医科大学
推荐引用方式 GB/T 7714	Liang, Qianqian,Wang, Baoyu,Pang, Lingxia,et al. Application of citrate as a tricarboxylic acid (TCA) cycle intermediate, prevents diabetic-induced heart damages in mice[J]. Iranian journal of basic medical sciences,2016,19(1):43-8..
APA	Liang, Qianqian., Wang, Baoyu., Pang, Lingxia., Wang, Youpei., Zheng, Meiqin., ... & Xu, Jinzhong. (2016). Application of citrate as a tricarboxylic acid (TCA) cycle intermediate, prevents diabetic-induced heart damages in mice. Iranian journal of basic medical sciences, 19(1), 43-8..
MLA	Liang, Qianqian,et al."Application of citrate as a tricarboxylic acid (TCA) cycle intermediate, prevents diabetic-induced heart damages in mice".Iranian journal of basic medical sciences 19.1(2016):43-8..