题名 | Application of citrate as a tricarboxylic acid (TCA) cycle intermediate, prevents diabetic-induced heart damages in mice |
作者 | |
发表日期 | 2016 |
发表期刊 | Iranian journal of basic medical sciences 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
关键词 | Citrate Diabetes Heart Nitration Tricarboxylic acid. Tricarboxylic acid |
其他关键词 | CARDIAC PATHOLOGICAL-CHANGES ; OXIDATIVE STRESS ; METALLOTHIONEIN ; CARDIOMYOPATHY ; ATP ; COMPLICATIONS ; MITOCHONDRIA ; INHIBITION ; ACTIVATION ; NITRATION |
摘要 | Objectives:Higher cellular reactive oxygen species (ROS) levels is important in reducing cellular energy charge (EC) by increasing the levels of key metabolic protein, and nitrosative modifications, and have been shown to damage the cardiac tissue of diabetic mice. However, the relation between energy production and heart function is unclear. Materials and methods:Streptozotocin (STZ, 150 mg/kg body weight) was injected intraperitoneally once to mice that had been fasted overnight for induction of diabetes. After diabetic induction, mice received citrate (5 µg/kg) through intraperitoneal injection every other day for 5 weeks. The caspase-3, plasminogen activator inhibitor 1 (PAI1), protein kinase B (PKB), commonly known as AKT and phosphorylated-AKT (p-AKT) proteins were examined to elucidate inflammation and apoptosis in the heart. For histological analysis, heart samples were fixed with 10% formalin and stained with hematoxylin-eosin (HE) and Sirius red to assess pathological changes and fibrosis. The expression levels[AGA1] of marker proteins, tyrosine nitration, activity of ATP synthase and succinyl-CoA3-ketoacid coenzyme A transferase-1 (SCOT), and EC were measured. Results:Intraperitoneal injection of citrate significantly reduced caspase-3 and PAI-1 protein levels and increased p-AKT level on the 5(th) week; EC in the heart was found to be increased as well. Further, the expression level, activity, and tyrosine nitration of ATP synthase and SCOT were not affected after induction of diabetes. Conclusion:Results indicate that application of citrate, a tricarboxylic acid (TCA) cycle intermediate, might alleviate cardiac dysfunction by reducing cardiac inflammation, apoptosis, and increasing cardiac EC. |
资助项目 | Western Michigan University[CZ5342]; |
出版者 | MASHHAD UNIV MED SCIENCES |
出版地 | MASHHAD |
ISSN | 2008-3866 |
EISSN | 2008-3874 |
卷号 | 19期号:1页码:43-8. |
页数 | 6 |
WOS类目 | Medicine, Research & Experimental ; Pharmacology & Pharmacy |
WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
WOS记录号 | WOS:000371470600007 |
收录类别 | PUBMED ; SCOPUS ; SCIE |
URL | 查看原文 |
Pubmed记录号 | 27096063 |
PMC记录号 | PMC4823615 |
Scopus记录号 | 2-s2.0-84969437840 |
通讯作者地址 | [Xu, Jinzhong]Wenling first people’s hospital,The Affiliated Hospital of Wenzhou Medical University,Wenling,317500,China |
scopus学科分类 | Biophysics;Biochemistry;Immunology and Microbiology (all);Drug Discovery;Clinical Biochemistry |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/55599 |
专题 | 基础医学院(机能实验教学中心)_基础医学实验教学中心_机能实验教学中心(挂靠、校级) 眼视光学院(生物医学工程学院)、附属眼视光医院 其他_附属温岭医院(温岭市第一人民医院) |
通讯作者 | Xu, Jinzhong |
作者单位 | 1.Department of Emergency,Zhengzhou Central Hospital,Zhengzhou University,Zhengzhou,450007,China; 2.Function Experiment Teaching Center,Wenzhou Medical University,Wenzhou,325305,China; 3.The Affiliated Eye Hospital,Wenzhou Medical University,Wenzhou,325000,China; 4.Department of Pain management,Wenzhou Hospital of integrated Chinese and Western Medicine,Wenzhou,325000,China; 5.Wenling First People’s Hospital,The Affiliated Hospital of Wenzhou Medical University,Wenling,317500,China |
通讯作者单位 | 温州医科大学 |
推荐引用方式 GB/T 7714 | Liang, Qianqian,Wang, Baoyu,Pang, Lingxia,et al. Application of citrate as a tricarboxylic acid (TCA) cycle intermediate, prevents diabetic-induced heart damages in mice[J]. Iranian journal of basic medical sciences,2016,19(1):43-8.. |
APA | Liang, Qianqian., Wang, Baoyu., Pang, Lingxia., Wang, Youpei., Zheng, Meiqin., ... & Xu, Jinzhong. (2016). Application of citrate as a tricarboxylic acid (TCA) cycle intermediate, prevents diabetic-induced heart damages in mice. Iranian journal of basic medical sciences, 19(1), 43-8.. |
MLA | Liang, Qianqian,et al."Application of citrate as a tricarboxylic acid (TCA) cycle intermediate, prevents diabetic-induced heart damages in mice".Iranian journal of basic medical sciences 19.1(2016):43-8.. |
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