The protective effect of ulinastatin on paraquat-induced injury in HK-2 cells and the underlying mechanisms

科研成果详情

题名	The protective effect of ulinastatin on paraquat-induced injury in HK-2 cells and the underlying mechanisms
作者	Xingrong She; Guangliang Hong; Jiaping Tan; Guangju Zhao; Mengfang Li; Zhongqiu Lu
发表日期	2015-07-01
发表期刊	Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Objective:To investigate the protective effect of ulinastatin (UTI) on HK-2 cells during paraquat (PQ)-induced injury and its underlying mechanisms. Methods:Routinely cultured HK-2 cells were divided into blank control group, PQ group, UTI+PQ group and UTI group. Cell viability was determined by CCK-8 assay. The concentration of PQ in HK-2 cells were measured by high performance liquid chromatography (HPLC). The production of total reactive oxygen species (ROS) were detected by fluorescence microscopy. The activities of superoxide dismutase activity (SOD) and the content of malondialdehyde (MDA) in HK-2 cells were observed by chemical colorimetry. The levels of tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA). Results:PQ, even at a dose of 200 µM, could significant suppress the viability of HK-2 cells in a dose-dependent and time-dependent. UTI showed no significant inhibitory effect on the viability of HK-2 cells when given at a dose below 8 000 U/ml (P > 0.05). Compared with the PQ group, the UTI+PQ group had significantly increased the viability of HK-2 cells in a dose-dependent of UTI (P < 0.05). Compared with the PQ group on the same hour, the UTI+PQ group showed decreased in PQ concentration in HK-2 cells (P < 0.05 for all except 6 h). Compared with the blank control group, the PQ group had significantly decreased SOD activity and significantly increased ROS level and MDA content (P < 0.05). Compared with the PQ group, the UTI+PQ group had significantly increased SOD activity and significantly decreased ROS level and MDA content (P < 0.05). Compared with the blank control group, the PQ group had significantly increased IL-6 and TNF-α level (P < 0.05); Compared with the PQ group, the UTI+PQ group had significantly decreased IL-6 and TNF-α level (P < 0.05). Conclusion:UTI significantly reduces the PQ-induced oxidative damage and inflammatory injury and its mechanism may be by reducing the accumulation of PQ in HK-2 cells.
ISSN	1001-9391
卷号	33 期号:7 页码:501-6.
收录类别	PUBMED ; SCOPUS
URL	查看原文
PubMed ID	26653645
SCOPUSEID	2-s2.0-84979796761
Scopus学科分类	Medicine (all)
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/55576
专题	附属第一医院_急诊科
作者单位	Emergency Department of the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
第一作者单位	附属第一医院_急诊科
第一作者的第一单位	附属第一医院_急诊科
推荐引用方式 GB/T 7714	Xingrong She,Guangliang Hong,Jiaping Tan,et al. The protective effect of ulinastatin on paraquat-induced injury in HK-2 cells and the underlying mechanisms[J]. Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases,2015,33(7):501-6..
APA	Xingrong She, Guangliang Hong, Jiaping Tan, Guangju Zhao, Mengfang Li, & Zhongqiu Lu. (2015). The protective effect of ulinastatin on paraquat-induced injury in HK-2 cells and the underlying mechanisms. Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases, 33(7), 501-6..
MLA	Xingrong She,et al."The protective effect of ulinastatin on paraquat-induced injury in HK-2 cells and the underlying mechanisms".Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 33.7(2015):501-6..