Study on the population-genetics of Mycobacterium tuberculosis from Sichuan Basin in China

科研成果详情

题名	Study on the population-genetics of Mycobacterium tuberculosis from Sichuan Basin in China
作者	Ying Li; Yuhong Fu; Mei Yuan; Lingling Dong; Hairong Huang; Weimin Li; Jimin Gao
发表日期	2015-04-01
发表期刊	Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 影响因子和分区
语种	英语
原始文献类型	Article
摘要	Objective:To explore the population-genetics characteristics of Mycobacterium tuberculosis (MTB) prevailing at the Sichuan basin of China. Methods:A total of 413 MTB strains collected from Sichuan basin were genotyped by large sequence polymorphism (LSP) and 15 loci variable number tandem repeat (VNTR). Difference between the distribution of lineage population was analyzed by χ(2)-test and the discriminatory ability of each VNTR locus was evaluated under the Hunter-Gaston Discriminatory Index (HGI). Both phylogeny on population level and genetic structure were demonstrated through N-J tree and the Minimal Spanning Tree (MST). Genetic differentiation of different lineage strains was analyzed by Analysis of Molecular Variance (AMOVA). Time of the Most Recent Common Ancestor (TMRCA) was calculated based on the Bayesian model. Results:Four hundred and thirteen MTBs were divided into two major lineages, in which the Beijing lineage accounted for 56.2% (232/413) and the Euro-American lineage for 43.8% (181/413). There was no significant difference of population distribution between the two lineages (P > 0.05). The N-J tree of Beijing lineage MTB presented distinctly "star-like" and 72.4% strains were grouped to one clonal complex in MST. The Euro-American lineages MTB presented "branch-like" in N-J tree and were grouped into multiple clonal complexes in MST. There was significant genetic differentiation in Beijing lineage MTBs between Chongqing and Sichuan (FST = 0.018 91, P < 0.05), but not in the Euro-American lineage MTB (FST = 0.005 19, P > 0.05). TMRCA of the largest clonal complex in Euro-American lineage MTB appeared to be 723 (95% CI: 517-946) years. Conclusion:Both Beijing lineage and Euro-American lineage MTBs were competitively prevalent in the Sichuan basin. There was some difference noticed between the two lineages referring to genetic differentiation. The invasion of Euro-American lineages MTB to the basin area might be associated with a war occurred in this area about 720 years ago.
ISSN	0254-6450
卷号	36 期号:4 页码:374-8.
收录类别	PUBMED ; SCOPUS
URL	查看原文
PubMed ID	25975553
SCOPUSEID	2-s2.0-84940988750
Scopus学科分类	Medicine (all)
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/55571
专题	检验医学院（生命科学学院、生物学实验教学中心）_模式生物技术与应用重点实验室
作者单位	Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035, China
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）_模式生物技术与应用重点实验室
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）_模式生物技术与应用重点实验室
推荐引用方式 GB/T 7714	Ying Li,Yuhong Fu,Mei Yuan,et al. Study on the population-genetics of Mycobacterium tuberculosis from Sichuan Basin in China[J]. Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi,2015,36(4):374-8..
APA	Ying Li., Yuhong Fu., Mei Yuan., Lingling Dong., Hairong Huang., ... & Jimin Gao. (2015). Study on the population-genetics of Mycobacterium tuberculosis from Sichuan Basin in China. Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi, 36(4), 374-8..
MLA	Ying Li,et al."Study on the population-genetics of Mycobacterium tuberculosis from Sichuan Basin in China".Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 36.4(2015):374-8..