Chronic Toxicological Effects of beta-Diketone Antibiotics on Zebrafish (Danio rerio) Using Transcriptome Profiling of Deep Sequencing

doi:10.1002/tox.22141

科研成果详情

题名	Chronic Toxicological Effects of beta-Diketone Antibiotics on Zebrafish (Danio rerio) Using Transcriptome Profiling of Deep Sequencing
作者	Wang, Huili1; Yin, Xiaohan 2; Li, Fanghui 2; Dahlgren, Randy A.3; Zhang, Yuna 2; Zhang, Hongqin1; Wang, Xuedong 2
发表日期	2016-11
发表期刊	ENVIRONMENTAL TOXICOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	transcriptome beta-diketone antibiotics zebrafish deep sequencing immune system
其他关键词	GLUTATHIONE ; TETRACYCLINE ; CONTAMINANTS ; APOPTOSIS ; TRANSPORT ; GENOME ; MRP1 ; TOOL
摘要	Transcriptome analysis is important for interpreting the functional elements of the genome and revealing the molecular constituents of cells and tissues. Herein, differentially transcribed genes were identified by deep sequencing after zebrafish (Danio rerio) were exposed to beta-diketone antibiotics (DKAs); 23,129 and 23,550 mapped genes were detected in control and treatment groups, a total of 3238 genes were differentially expressed between control and treatment groups. Of these genes, 328 genes (213 up- and 115 down-regulation) had significant differential expression (p < 0.05) and an expression ratio (control/treatment) of >2 or <0.5. Additionally, we performed Gene Ontology (GO) category and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses, and found 266 genes in the treatment group with annotation terms linked to the GO category. A total of 77 differentially expressed transcriptional genes were associated with 132 predicted KEGG metabolic pathways. Serious liver tissue damage was reflected and consistent with the differences in genetic classification and function from the transcriptome analysis. These results enhance our understanding of zebrafish developmental processes under exposure to DKA stress. (C) 2015 Wiley Periodicals, Inc.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270548, 21377100]; Zhejiang Provincial Environmental Protection Department [2012B014]
出版者	WILEY
出版地	HOBOKEN
ISSN	1520-4081
EISSN	1522-7278
卷号	31 期号:11 页码:1357-1371
DOI	10.1002/tox.22141
页数	40
WOS类目	Environmental Sciences ; Toxicology ; Water Resources
WOS研究方向	Environmental Sciences & Ecology ; Toxicology ; Water Resources
WOS记录号	WOS:000388616700007
收录类别	SCIE ; PUBMED ; EI ; SCOPUS
EI入藏号	20151500733274
EI主题词	Genes
URL	查看原文
PubMed ID	25846866
SCOPUSEID	2-s2.0-84926443189
通讯作者地址	[Wang, Xuedong]Department of Environmental Sciences,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类	Toxicology;Management, Monitoring, Policy and Law;Health, Toxicology and Mutagenesis
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/5408
专题	检验医学院（生命科学学院、生物学实验教学中心）_生物化学与分子生物学系公共卫生学院_环境科学系
通讯作者	Wang, Xuedong
作者单位	1.Department of biochemistry and molecular biology,College of Life Sciences,Wenzhou Medical University,Wenzhou,325035,China; 2.Department of Environmental Sciences,Wenzhou Medical University,Wenzhou,325035,China; 3.Department of Land,Air and Water Resources,University of California,Davis,95616,United States
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	温州医科大学
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Wang, Huili,Yin, Xiaohan,Li, Fanghui,et al. Chronic Toxicological Effects of beta-Diketone Antibiotics on Zebrafish (Danio rerio) Using Transcriptome Profiling of Deep Sequencing[J]. ENVIRONMENTAL TOXICOLOGY,2016,31(11):1357-1371.
APA	Wang, Huili., Yin, Xiaohan., Li, Fanghui., Dahlgren, Randy A.., Zhang, Yuna., ... & Wang, Xuedong. (2016). Chronic Toxicological Effects of beta-Diketone Antibiotics on Zebrafish (Danio rerio) Using Transcriptome Profiling of Deep Sequencing. ENVIRONMENTAL TOXICOLOGY, 31(11), 1357-1371.
MLA	Wang, Huili,et al."Chronic Toxicological Effects of beta-Diketone Antibiotics on Zebrafish (Danio rerio) Using Transcriptome Profiling of Deep Sequencing".ENVIRONMENTAL TOXICOLOGY 31.11(2016):1357-1371.