姜黄素对高血压病大鼠全脑缺血再灌注损伤时海马炎性反应的影响

doi:10.3760/cma.j.issn.0254-1416.2014.06.031

科研成果详情

题名	姜黄素对高血压病大鼠全脑缺血再灌注损伤时海马炎性反应的影响
其他题名	Effect of curcumin on inflammatory responses in hippocampus during global cerebral ischemia- reperfusion injury in hypertensive rats
作者	汪小丹; 沈露露; 俞陈陈; 曹红; 姬斌; 李军
发表日期	2014-06-20
发表期刊	中华麻醉学杂志影响因子和分区
语种	中文
原始文献类型	学术期刊
关键词	姜黄素高血压炎症脑再灌注损伤海马
其他关键词	Curcumin ; Hypertension ; Inflammation ; Brain ; Reperfusion injury ; Hippocampus
摘要	目的评价姜黄素对高血压病大鼠全脑缺血再灌注损伤时海马炎性反应的影响。方法选择SPF级雄性Wistar-Kyoto大鼠48只,8周龄,体重180~200 g,采用随机数字表法,将其分为2组(n=24):假手术组(W-Sham组)、缺血再灌注组(W-I/R组)。SPF级雄性自发性高血压(SH)大鼠72只,8周龄,体重180~200 g,采用随机数字表法,将其分为3组(n=24):假手术组(S-Sham组)、缺血再灌注组(S-I/R组)、姜黄素组(S-Cur组)。采用四血管阻断法建立全脑缺血再灌注损伤模型(缺血10min后恢复灌注)。S-Cur组于再灌注30 min时腹腔注射姜黄素100 mg/kg,W-Sham组、S-Sham组、W-I/R组和S-I/R组于再灌注30 min时腹腔注射玉米油5 ml/kg。于再灌注7 d时行跳台实验测定学习和记忆成绩,于再灌注3 h、1、3和7 d(T_(1-4))时处死取海马组织,苏木素-伊红染色后观察海马CA1区锥体细胞形态,Nissl染色后计数海马CA1区平均锥体细胞密度,ELISA法测定海马IL-1β、TNF-α和IL-10的含量。结果与W-Sham组比较,W-I/R组学习和记忆成绩降低,海马CA1区平均锥体细胞密度减少,T_(1-3)时海马IL-1β含量升高,T_1时海马TNF-α含量升高,海马IL-10含量于T_2时升高,T_(1,3,4)时降低(P<0.05),S-Sham组上述各指标比较差异无统计学意义(P>0.05);与W-I/R组比较,S-I/R组学习和记忆成绩降低,T_(1,3)时海马IL-1β含量降低,T_1时海马TNF-α含量升高,T_2时海马IL-10含量降低(P<0.05),海马CA1区平均锥体细胞密度差异无统计学意义(P>0.05),海马CA1区锥体细胞损伤严重;与S-I/R组比较,S-Cur组学习和记忆成绩升高,海马CA1区平均锥体细胞密度增多,T_(2,3)时海马IL-1β含量降低,T_(1-4)时海马TNF-α含量降低,T_2时海马IL-10含量升高(P<0.05),海马CA1区锥体细胞损伤减轻。结论姜黄素减轻高血压病大鼠全脑缺血再灌注损伤的机制可能与抑制海马炎性反应有关。
其他摘要	Objective To evaluate the effect of curcumin on the inflammatory responses in the hippocampus during global cerebral ischemia-reperfusion (I/R) injury in hypertensive rats.Methods Forty-eight SPF male Wistar-Kyoto rats, aged 8 weeks, weighing 180-200 g, were randomly divided into 2 groups (n = 24 each) using a random number table: sham operation group (W-Sham group) and I/R group (W-I/R group). Seventy-two SPF male spontaneously hypertensive rats, aged 8 weeks, weighing 180-200 g, were randomly divided into 3 groups (n = 24 each) using a random number table: sham operation group (S-Sham group),I/R group (SI/R group), and curcumin group (S-Cur group). Global cerebral ischemia was induced by 4-vessel occlusion method (10 min of transient global ischemia followed by reperfusion). Curcumin 100 mg/kg was injected intraperitoneally at 30 min of reperfusion in S-Cur group, and corn oil 5 ml/kg was injected intraperitoneally at 30 min of reperfusion in the other groups. The ability of learning and memory was tested by step-down test at 7 days of reperfusion. Rats were sacrificed at 3 h and 1,3 and 7 days of reperfusion (T_(1-4)) and the hippocampi were removed. The morphological changes of pyramidal cells in hippocampal CA1 area were observed by HE staining. The mean density of pyramidal cells in hippocampal CA1 area was quantified by Nissl staining. The contents of interleukin-lp (IL-1β),tumor necrosis factor-alpha (TNF-α) and 11,-10 in the hippocampus were determined by ELISA. Results Compared with W-Sham group, the ability of learning and memory was significantly decreased, the mean density of pyramidal cells in hippocampal CA1 area was decreased, the contents of IL-1β at T_(1-3),TNF-α at T_1,and IL-10 at T_2 were increased, and the contents of IL-10 were decreased at T_(1,3,4) in W-I/R group, and no significant changes were found in the parameters mentioned above in S-Sham group. Compared with W-I/R group, the ability of learning and memory was significantly decreased, the contents of IL-lβ at T_(1,3) and IL-10 at T_2 were decreased, the content of TNF-α was increased at T_1,no significant change was found in the mean density of pyramidal cells in hippocampal CA1 area, and the damage to pyramidal cells in hippocampal CA1 area was severe in S-I/R group. Compared with S-I/R group, the ability of learning and memory was significantly increased, the mean density of pyramidal cells in hippocampal CA1 area was increased, the contents of IL-1β at T_(2,3) and TNF-α at T_(1-4) were increased, and the content of IL-10 was increased at T_2,and the damage to pyramidal cells in hippocampal CA1 area was reduced in S-Cur group. Conclusion Inhibition of inflammatory responses in the hippocampus may be involved in the mechanism by which curcumin reduces global cerebral I/R injury in hypertensive rats.
资助项目	浙江省科技厅公益类项目（2011C23022）
ISSN	0254-1416
卷号	34 期号:06 页码:753-757
DOI	10.3760/cma.j.issn.0254-1416.2014.06.031
页数	5
收录类别	CNKI ; 万方 ; 维普 ; 北大核心 ; ISTIC ; CSCD ; PKU
学科领域	医药、卫生 ; 神经病学与精神病学
URL	查看原文
CSCD记录号	CSCD:5219412
维普分类号	R285.5
CN号	13-1073/R
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/51684
专题	温州医科大学
作者单位	温州医科大学附属第二医院麻醉科
推荐引用方式 GB/T 7714	汪小丹,沈露露,俞陈陈,等. 姜黄素对高血压病大鼠全脑缺血再灌注损伤时海马炎性反应的影响[J]. 中华麻醉学杂志,2014,34(06):753-757.
APA	汪小丹, 沈露露, 俞陈陈, 曹红, 姬斌, & 李军. (2014). 姜黄素对高血压病大鼠全脑缺血再灌注损伤时海马炎性反应的影响. 中华麻醉学杂志, 34(06), 753-757.
MLA	汪小丹,et al."姜黄素对高血压病大鼠全脑缺血再灌注损伤时海马炎性反应的影响".中华麻醉学杂志 34.06(2014):753-757.