COMPARISON OF SALT-TOLERANCE AND GENETIC EVOLUTIONARY ANALYSIS FOR FIVE ISOLATES OF Spirulina platensis FROM CONTRASTING ECOLOGICAL ENVIRONMENTS

科研成果详情

题名	COMPARISON OF SALT-TOLERANCE AND GENETIC EVOLUTIONARY ANALYSIS FOR FIVE ISOLATES OF Spirulina platensis FROM CONTRASTING ECOLOGICAL ENVIRONMENTS
作者	Zeng, Aibing 1; Zhu, Junlan 1; Zhao, Xiaokai 1; Wang, Huili1; Li, Aiying 2; Dahlgren, Randy A.3; Yan, Hao 1; Wang, Xuedong 3
发表日期	2013-12-31
发表期刊	FRESENIUS ENVIRONMENTAL BULLETIN 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Arthrospira (Spirulina) platensis salt-tolerance cluster analysis phylogenetic tree internal transcribed spacer
其他关键词	16S RIBOSOMAL-RNA ; MOLECULAR MARKERS ; CYANOBACTERIA ; PROTEINS ; SUPPORT ; GROWTH
摘要	Five strains of Spirulina platensis were collected from contrasting ecological environments and investigated for their salt-tolerance and evolutionary relationship. First, we analyzed the differentially expressed protein profiles of the Spi-yz strain (Qingdao seashore, China) cultivated between 0.02 and 1 M NaCl concentration. Then, we selected six positive proteins from the Spi-yz strain whose expression was up-regulated by greater than 3-fold at the higher NaCl concentration. The ORFs for the six up-regulated proteins related to salt-tolerance of Spi-yz were ORF4201, ORF1368, ORF1074, ORF4938, ORF4554 and ORF4634. Subsequently, we amplified full-length ORFs of the six genes using total DNA from four other Spirulina strains as a template. The four Spirulina strains were FACHB-350 (Texas, USA), FACHB-793 (Jiangxi, China), FACHB-834 (Dongying salt marsh, China) and FACHB-904 (Guangdong seashore, China). The COG functional classification of the six up-regulated proteins belongs to putative D-alanyl-D-alanine dipeptidase, redoxin domain protein, EF-Ts, glutathione synthetase, phycocyanin alpha subunit, and phage shock protein A in protein MALDI-TOF/MS identification database, respectively. The homology of the six genes in different Spirulina strains was in the range 83-99% for ORF1074, 85-99% for ORF4938, 79-99% for ORF1368, 85-99% for ORF4634, 85-97% for ORF4554, and 84-99% for ORF4201. Higher homology was observed in four genes (ORF1074, ORF4938, ORF1368 and ORF4634) of the FACHB-793, FACHB-834 and FACHB-904 strains. The similar salt-tolerance characteristics of FACHB-793 and FACHB-904 suggest that the four genes might be closely related to salt-resistant response. In addition, UPGMA cluster analysis was performed to evaluate the different morphological characteristics and cultural traits of the five Spirulina strains and to confirm the six genes related to salt-resistant response. Finally, a phylogenetic tree was constructed from the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer (ITS) homology analysis to explore their phylogenetic relationship. Differences in salt-tolerance and morphological features for the five Spirulina strains had a close relationship with their long-term geographical environment. As a reflection of genetic evolution, their salt-resistance ability and morphological characteristics are consistent with their corresponding molecular evolution.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31071115]; Zhejiang Provincial Natural Science FoundationNatural Science Foundation of Zhejiang Province [Y3110050, LY13B070011]; Initial Foundation of Wenzhou Medical College [QTJ13006]; Zhejiang Provincial Xinmiao Talent Project [2012R413031]; Zhejiang Environmental Protection Department [2012B014]
出版者	PARLAR SCIENTIFIC PUBLICATIONS (P S P)
出版地	FREISING
ISSN	1018-4619
EISSN	1610-2304
卷号	22 期号:6 页码:1697-1709
页数	13
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
WOS记录号	WOS:000320901100007
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-84880062615
通讯作者地址	[Wang, Huili]College of Life Sciences,Wenzhou Medical College,University-Town,China
Scopus学科分类	Environmental Chemistry;Waste Management and Disposal;Pollution
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/4321
专题	检验医学院（生命科学学院、生物学实验教学中心）仁济学院_环境与公共卫生学部
通讯作者	Wang, Huili
作者单位	1.College of Life Sciences,Wenzhou Medical College,University-Town,China; 2.College of Life Sciences,Central China Normal University,China; 3.College of Environmental Science and Public Health,Wenzhou Medical College,University-Town,China
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Zeng, Aibing,Zhu, Junlan,Zhao, Xiaokai,et al. COMPARISON OF SALT-TOLERANCE AND GENETIC EVOLUTIONARY ANALYSIS FOR FIVE ISOLATES OF Spirulina platensis FROM CONTRASTING ECOLOGICAL ENVIRONMENTS[J]. FRESENIUS ENVIRONMENTAL BULLETIN,2013,22(6):1697-1709.
APA	Zeng, Aibing., Zhu, Junlan., Zhao, Xiaokai., Wang, Huili., Li, Aiying., ... & Wang, Xuedong. (2013). COMPARISON OF SALT-TOLERANCE AND GENETIC EVOLUTIONARY ANALYSIS FOR FIVE ISOLATES OF Spirulina platensis FROM CONTRASTING ECOLOGICAL ENVIRONMENTS. FRESENIUS ENVIRONMENTAL BULLETIN, 22(6), 1697-1709.
MLA	Zeng, Aibing,et al."COMPARISON OF SALT-TOLERANCE AND GENETIC EVOLUTIONARY ANALYSIS FOR FIVE ISOLATES OF Spirulina platensis FROM CONTRASTING ECOLOGICAL ENVIRONMENTS".FRESENIUS ENVIRONMENTAL BULLETIN 22.6(2013):1697-1709.