LINC00518 affects the proliferation, invasion and migration of cutaneous malignant melanoma cells via miR-526b-3p/EIF5A2 axis

doi:10.18388/abp.2020_5746

科研成果详情

题名	LINC00518 affects the proliferation, invasion and migration of cutaneous malignant melanoma cells via miR-526b-3p/EIF5A2 axis
作者	Xu, Jie 1; Zhang, Fan 2; Lin, Manman 1; Tong, Yun 1
发表日期	2022
发表期刊	ACTA BIOCHIMICA POLONICA 影响因子和分区
语种	英语
原始文献类型	Article
关键词	cutaneous malignant melanoma LINC00518 MiR-526b-3p proliferation invasion migration
其他关键词	HOXD-AS1 PROMOTES ; COLORECTAL-CANCER ; EIF5A2 ; PROGRESSION
摘要	Objective: To investigate the mechanism of LINC00518 affecting the proliferation, invasion, and migration of cu-taneous malignant melanoma (CMM) cells via miR-526b-3p/EIF5A2 axis. Methods: qRT-PCR was performed to measure the expression of LINC00518, miR-526b-3p, and EIF5A2 in CMM tissues from 40 patients. Si-LINC00518, pcDNA-LINC00518, miR-526b-3p mimic, miR-526b-3p in-hibitor, si-EIF5A2, and their corresponding negative con-trols were transfected alone or co-transfected into CMM cells A375 and A2058. The expression of LINC00518, miR-526b-3p and EIF5A2 in A375 and A2058 cells was measured. Cell proliferation was tested by CCK-8 assay and EdU assay. Cell invasion and migration were de-tected by Transwell and scratch tests, respectively. The binding between LINC00518 and miR-526b-3p, and the binding between miR-526b-3p and EIF5A2 were verified by dual-luciferase reporter and RNA pull-down assays. Results: LINC00518 and EIF5A2 were up-regulated and miR-526b-3p was down-regulated in CMM tissues and cells. CMM patients with highly expressed LINC00518 showed decreased survival time than those with lowly expressed LINC00518. Transfection of si-LINC00518, miR-526b-5p mimic or si-EIF5A2 weakened the prolif-erative, migratory, and invasive abilities of melanoma cells, while transfection of miR-526b-5p inhibitor or pcDNA-LINC00518 enhanced the progression of mela-noma cells. Moreover, the proliferative, migratory, and invasive potentials of melanoma cells were decreased after co-transfection of si-EIF5A2 and pcDNA-LINC00518 compared with cells transfected with pcDNA-LINC00518 alone. LINC00518 bound to miR-526b-3p and miR-526b-3p targeted EIF5A2. LINC00518 negatively regulated miR-526b-3p expression but positively regulated EIF5A2. Furthermore, EIF5A2 expression was negatively associat-ed with miR-526b-3p expression. Conclusion: LINC00518 encourages CMM through the miR-526b-3p/EIF5A2 axis in terms of cell proliferation, invasion, and migration.
出版者	ACTA BIOCHIMICA POLONICA
出版地	WARSAW
ISSN	0001-527X
EISSN	1734-154X
卷号	69 期号:1 页码:101-111
DOI	10.18388/abp.2020_5746
页数	11
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
WOS记录号	WOS:000759554800001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	35189053
SCOPUSEID	2-s2.0-85125682923
通讯作者地址	[Tong, Yun]Plastic and Cosmetic Center,Affiliated Jinhua Hospital,Zhejiang University School of Medicine,Zhejiang,Jinhua,321000,China
Scopus学科分类	Biochemistry, Genetics and Molecular Biology (all)
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/4193
专题	附属第一医院
通讯作者	Tong, Yun
作者单位	1.Plastic and Cosmetic Center,Affiliated Jinhua Hospital,Zhejiang University School of Medicine,Zhejiang,Jinhua,321000,China; 2.Department of Dermatology,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China
推荐引用方式 GB/T 7714	Xu, Jie,Zhang, Fan,Lin, Manman,et al. LINC00518 affects the proliferation, invasion and migration of cutaneous malignant melanoma cells via miR-526b-3p/EIF5A2 axis[J]. ACTA BIOCHIMICA POLONICA,2022,69(1):101-111.
APA	Xu, Jie, Zhang, Fan, Lin, Manman, & Tong, Yun. (2022). LINC00518 affects the proliferation, invasion and migration of cutaneous malignant melanoma cells via miR-526b-3p/EIF5A2 axis. ACTA BIOCHIMICA POLONICA, 69(1), 101-111.
MLA	Xu, Jie,et al."LINC00518 affects the proliferation, invasion and migration of cutaneous malignant melanoma cells via miR-526b-3p/EIF5A2 axis".ACTA BIOCHIMICA POLONICA 69.1(2022):101-111.