Apelin-13 Administration Protects Against LPS-Induced Acute Lung Injury by Inhibiting NF-kappa B Pathway and NLRP3 Inflammasome Activation

doi:10.1159/000493653

科研成果详情

题名	Apelin-13 Administration Protects Against LPS-Induced Acute Lung Injury by Inhibiting NF-kappa B Pathway and NLRP3 Inflammasome Activation
作者	Zhang, Hailin1,2; Chen, Sha3; Zeng, Meichun 4; Lin, Daopeng 1,2; Wang, Yu 3; Wen, Xunhang 1,2; Xu, Changfu1,2; Yang, Li5; Fan, Xiaofang3; Gong, Yongsheng3; Zhang, Hongyu6; Kong, Xiaoxia3
发表日期	2018-12-31
发表期刊	CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Acute lung injury (ALI) Lipopolysaccharide (LPS) Apelin-13 NF-kappa B NLRP3
其他关键词	ORPHAN RECEPTOR APJ ; ALVEOLAR MACROPHAGES ; CARDIAC CONTRACTILITY ; ENDOTHELIAL-CELLS ; ENDOGENOUS LIGAND ; EPITHELIAL-CELLS ; PEPTIDE APELIN ; IN-VIVO ; CONTRIBUTES ; EXPRESSION
摘要	Background/Aims: Acute lung injury (ALI) is induced by a variety of external and internal factors and leads to acute progressive respiratory failure. Previous studies have shown that apelin-13 can decrease the acute lung injury induced by LPS, but the specific mechanism is unclear. Therefore, a mouse lung injury model and a cell model were designed to explore the mechanism of how apelin-13 alleviates the acute lung injury caused by LPS. Methods: The effect of apelin-13 on LPS-induced structural damage was determined by H&E staining and by the wet/dry weight ratio. The related inflammatory factors in BALF were examined by ELISA. The apoptotic pathway and the NF-kappa B and NLRP3 inflammasome pathways were evaluated by using Western blotting and immunofluorescence staining. Results: LPS induced the structural damage and the production of inflammatory cytokines in the lung tissues of mice. These deleterious effects were attenuated by apelin-13 administration. The protective effects of apelin-13 were associated with decreased reactive oxygen species (ROS) formation and the inhibition of the activation of the NF-kappa B and NLRP3 inflammasome pathways in mice and in Raw264.7 cells. Conclusion: Taken together, these data suggest that apelin-13 administration ameliorates LPS-induced acute lung injury by suppressing ROS formation, as well as by inhibiting the NF-kappa B pathway and the activation of the NLRP3 inflammasome in the lungs. (C) 2018 The Author(s) Published by S. Karger AG, Basel
资助项目	Zhejiang Provincial Natural Science Funding [LY17H010009, LY17H010005, Y17H160193]; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81772450, 81472165, 81600033]
出版者	KARGER
出版地	BASEL
ISSN	1015-8987
EISSN	1421-9778
卷号	49 期号:5 页码:1918-1932
DOI	10.1159/000493653
页数	15
WOS类目	Cell Biology ; Physiology
WOS研究方向	Cell Biology ; Physiology
WOS记录号	WOS:000447182000019
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	30235451
SCOPUSEID	2-s2.0-85053861508
通讯作者地址	[Zhang, Hongyu;Kong, Xiaoxia]Wenzhou Med Univ, Inst Hypoxia Res, Wenzhou 325035, Zhejiang, Peoples R China.
Scopus学科分类	Medicine (all)
TOP期刊	TOP期刊
引用统计	被引频次：59[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/4135
专题	第二临床医学院、附属第二医院、育英儿童医院基础医学院（机能实验教学中心）药学院（分析测试中心）附属第二医院附属第一医院附属第二医院_儿童呼吸科附属第一医院_呼吸内科
通讯作者	Zhang, Hongyu; Kong, Xiaoxia
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 2, Dept Childrens Respirat, Wenzhou, Peoples R China; 2.Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou, Peoples R China; 3.Wenzhou Med Univ, Inst Hypoxia Res, Sch Basic Med Sci, Wenzhou, Peoples R China; 4.Zhejiang Univ, Coll Med, Affiliated Hosp 1, Dept Pathol, Hangzhou, Zhejiang, Peoples R China; 5.Wenzhou Med Univ, Affiliated Hosp 1, Dept Resp Med, Wenzhou, Peoples R China; 6.Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou, Peoples R China
第一作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院; 儿童呼吸科
通讯作者单位	温州医科大学
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Zhang, Hailin,Chen, Sha,Zeng, Meichun,et al. Apelin-13 Administration Protects Against LPS-Induced Acute Lung Injury by Inhibiting NF-kappa B Pathway and NLRP3 Inflammasome Activation[J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2018,49(5):1918-1932.
APA	Zhang, Hailin., Chen, Sha., Zeng, Meichun., Lin, Daopeng., Wang, Yu., ... & Kong, Xiaoxia. (2018). Apelin-13 Administration Protects Against LPS-Induced Acute Lung Injury by Inhibiting NF-kappa B Pathway and NLRP3 Inflammasome Activation. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 49(5), 1918-1932.
MLA	Zhang, Hailin,et al."Apelin-13 Administration Protects Against LPS-Induced Acute Lung Injury by Inhibiting NF-kappa B Pathway and NLRP3 Inflammasome Activation".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 49.5(2018):1918-1932.