Macrolide-Resistant Mycoplasma pneumoniae in Adults in Zhejiang, China

doi:10.1128/AAC.04308-14

科研成果详情

题名	Macrolide-Resistant Mycoplasma pneumoniae in Adults in Zhejiang, China
作者	Zhou, Zibo1; Li, Xiangzhi 1; Chen, Xiaojian 2; Luo, Fangjun 3; Pan, Changwang 1; Zheng, Xiaoping 3; Tan, Feng1
发表日期	2015-02
发表期刊	ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	COMMUNITY-ACQUIRED PNEUMONIA ; REAL-TIME PCR ; LABORATORY DIAGNOSIS ; NESTED PCR ; INFECTION ; CHILDREN ; STRAINS ; ELECTROPHORESIS ; SUSCEPTIBILITY ; PREVALENCE
摘要	Mycoplasma pneumoniae is a major pathogen causing community-acquired pneumoniae (CAP), which is generally treated with macrolides. In recent years, however, although macrolide-resistant M. pneumoniae has been reported frequently, particularly in China, very little is known about the prevalence of macrolide-resistant M. pneumoniae infection in adults. In this study, we survey the macrolide-resistant M. pneumoniae in adults in Zhejiang province and characterize the mechanisms of resistance to macrolide. Six hundred fifty throat swab samples were collected from adult patients with CAP from January 2012 to August 2014. These samples were assayed by nested PCR and then cultivated for M. pneumoniae. All isolates were sequenced to determine the mutation in domain V of the 23S rRNA gene. The activities of 10 antibiotics against macrolide-resistant M. pneumoniae isolates were also investigated in vitro. Moreover, restriction fragment length polymorphism (RFLP) analysis of the amplified P1 gene was used to type 50 resistant strains. One hundred percent (71/71) of M. pneumoniae strains isolated from adults with CAP were resistant to erythromycin (MIC = 128 to >256 mu g/ml), clarithromycin (MIC = 128 to >256 mu g/ml), and azithromycin (MIC = 32 to >64 mu g/ml). Furthermore, all macrolide-resistant M. pneumoniae strains identified had an A2063G mutation in domain V of the 23S rRNA gene. Forty-six resistant strains (92.0%) were classified into type I strain on the basis of P1 gene PCR-RFLP analysis. According to these findings, it is suggested that macrolide-resistant M. pneumoniae infection is very prevalence among adults in Zhejiang province. Thus, there is necessary to perform the epidemiological monitoring of macrolide-resistant M. pneumoniae in the future.
资助项目	Social Development Program of Science and Technology Department of Zhejiang Province of China [2011C13040]
出版者	AMER SOC MICROBIOLOGY
出版地	WASHINGTON
ISSN	0066-4804
EISSN	1098-6596
卷号	59 期号:2 页码:1048-1051
DOI	10.1128/AAC.04308-14
页数	4
WOS类目	Microbiology ; Pharmacology & Pharmacy
WOS研究方向	Microbiology ; Pharmacology & Pharmacy
WOS记录号	WOS:000348610000036
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	25451048
PMC记录号	PMC4335877
SCOPUSEID	2-s2.0-84921790150
通讯作者地址	[Pan, Changwang]Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University,Wenzhou, Zhejiang,China
Scopus学科分类	Pharmacology;Pharmacology (medical);Infectious Diseases
TOP期刊	TOP期刊
引用统计	被引频次：49[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/3615
专题	基础医学院（机能实验教学中心）附属第二医院第二临床医学院，附属第二医院、育英儿童医院第二临床医学院，附属第二医院、育英儿童医院_临床检验诊断学
通讯作者	Pan, Changwang
作者单位	1.Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University,Wenzhou, Zhejiang,China; 2.Department of Clinical Laboratory, Second Affiliated Hospital of Wenzhou Medical University,Wenzhou, Zhejiang,China; 3.Department of Clinical Laboratory, Zhuji People's Hospital,Zhuji, Zhejiang,China
第一作者单位	基础医学院（机能实验教学中心）
通讯作者单位	基础医学院（机能实验教学中心）
第一作者的第一单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	Zhou, Zibo,Li, Xiangzhi,Chen, Xiaojian,et al. Macrolide-Resistant Mycoplasma pneumoniae in Adults in Zhejiang, China[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,2015,59(2):1048-1051.
APA	Zhou, Zibo., Li, Xiangzhi., Chen, Xiaojian., Luo, Fangjun., Pan, Changwang., ... & Tan, Feng. (2015). Macrolide-Resistant Mycoplasma pneumoniae in Adults in Zhejiang, China. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 59(2), 1048-1051.
MLA	Zhou, Zibo,et al."Macrolide-Resistant Mycoplasma pneumoniae in Adults in Zhejiang, China".ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 59.2(2015):1048-1051.