Whole CagA gene amplification of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism

科研成果详情

题名	Whole CagA gene amplification of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism
作者	Ye, Siying 1; Ao, Jienan 2; Peng, Ying3; Yue, Haifeng 4; Liao, Fang 1; Hu, Guoping 4; Xu, Yang 1; Zhang, Zhengmao 1
发表日期	2002
发表期刊	Journal of Huazhong University of Science and Technology - Medical Science 影响因子和分区
语种	英语
原始文献类型	Journal article (JA)
关键词	Biochemistry DNA DNA fragments Fingerprinting PCR
摘要	To set up a method of amplification for the whole CagA gene of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism(RFLP), nested PCR was employed in combination with TD-PCR to amplify the gene and EcoRI and Hind I were used to generate the RFLP fingerprinting. Target DNA fragments from 13 of 20 samples were successfully amplified and the relevant RFLP fingerprintings were obtained. It is concluded that the method can be used to amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile.
出版者	Tongji Medical University
ISSN	0257-716X
卷号	22 期号:4 页码:276-278
页数	7
收录类别	EI ; SCOPUS
EI入藏号	2005469470974
EI主题词	Genes
URL	查看原文
PubMed ID	12674756
SCOPUSEID	2-s2.0-3042833001
通讯作者地址	[Ye, Siying]Department of Pathogenic Organisms,School of Basic Medical Sciences,Huazhong University of Science and Technology,China
Scopus学科分类	Biochemistry;Biomaterials;Biomedical Engineering;Genetics
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/34857
专题	检验医学院（生命科学学院、生物学实验教学中心）_细胞与分子生物医学研究所
通讯作者	Ye, Siying
作者单位	1.Department of Pathogenic Organisms,School of Basic Medical Sciences,Huazhong University of Science and Technology,China; 2.Medical College of Jinan University,China; 3.Center of Cellular and Molecular Biology,Wenzhou Medical College,China; 4.Department of Microbiology And Infectious Diseases,University of Calgary,Faculty of Medicine,Canada
推荐引用方式 GB/T 7714	Ye, Siying,Ao, Jienan,Peng, Ying,et al. Whole CagA gene amplification of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism[J]. Journal of Huazhong University of Science and Technology - Medical Science,2002,22(4):276-278.
APA	Ye, Siying., Ao, Jienan., Peng, Ying., Yue, Haifeng., Liao, Fang., ... & Zhang, Zhengmao. (2002). Whole CagA gene amplification of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism. Journal of Huazhong University of Science and Technology - Medical Science, 22(4), 276-278.
MLA	Ye, Siying,et al."Whole CagA gene amplification of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism".Journal of Huazhong University of Science and Technology - Medical Science 22.4(2002):276-278.