[Effects of electroacupuncture on activation of microglia cells in spinal cord in rats with neuropathic pain]

doi:10.13703/j.0255-2930.2017.04.016

科研成果详情

题名	[Effects of electroacupuncture on activation of microglia cells in spinal cord in rats with neuropathic pain]
其他题名	Effects of electroacupuncture on activation of microglia cells in spinal cord in rats with neuropathic pain
作者	Sisi Li; Pengpeng Gu; Wenzhan Tu; Xia Jiang; Wenci Chen; Qimiao Hu; Songhe Jiang
发表日期	2017-04-12
发表期刊	Zhongguo zhen jiu = Chinese acupuncture &moxibustion 影响因子和分区
语种	英语
原始文献类型	学术期刊
关键词	acupoint, GB34 (Yanglingquan) acupoint, ST36 (Zusanli) brain-derived neurotrophic factor (BDNF) electroacupuncture (EA) microglial cells neuropathic pain.
其他关键词	neuropathic pain ; electroacupuncture (EA) ; microglial cells ; brain-derived neurotrophic factor (BDNF) ; acupoint ; ST36 (Zusanli) ; GB34 (Yanglingquan)
摘要	Objective:To observe the effects of electroacupuncture (EA) on the activation of microglia cells in the L4 to L6 spinal cord in rats with neuropathic pain, so as to investigate whether EA could inhibit the activation of spinal microglial cells and regulate the expression of brain-derived neurotrophic factor (BDNF) to achieve the analgesic effect. Methods:Forty male Sprague Dawley rats were randomly divided into a normal group, a sham-model group, a model group and an EA group, 10 rats in each one. The rats in the normal group received no treatment; the rats in sham-model group were treated with operation to exposure sciatic nerve for 2 to 3 min (no knot); the rats in the remaining groups were treated with model establishment of chronic constrictive injury (CCI). 7 days after model establishment, the rats in the EA group were treated with EA at "Zusanli" (ST 36) and "Yanglingquan" (GB 34), 30 min per time, once a day for consecutive 7 days. Only immobilization was used in the remaining groups the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) of affected side feet were respectively measured before model establishment and 3 days, 5 days, 7 days, 10 days, 12 days and 14 days after model establishment; 14 days after model establishment, rats were sacrificed; the immunohistochemical method was used to measure the expression of Iba1 and BDNF in the sample of L4 to L6 spinal cord; real-time fluorescent quantitative PCR was used to measure the expression BDNF mRNA. Results:Compared with the sham-model group, the pain threshold was decreased significantly in the model group (P<0.05), leading to hyperpathia. After EA treatment, compared with the model group, the pain threshold was increased significantly in the EA group (P<0.05). 14 days after operation, the microglia cells in the L4 to L6 spinal cord, expression of BDNF and level of mRNA in the model group were significantly higher than those in the normal group and sham-model group (all P<0.01); those in the EA group were significantly lower than those in the model group (all P<0.01). Conclusions:The analgesic effect on neuropathic pain is likely to be achieved by EA through inhibiting the activation of spinal microglia cells and down-regulating the expression of BDNF.
其他摘要	Objective To observe the effects of electroacupuncture (EA) on the activation of microglia cells in the L_4 to L_6 spinal cord in rats with neuropathic pain, so as to investigate whether EA could inhibit the activation of spinal microglial cells and regulate the expression of brain-derived neurotrophic factor (BDNF) to achieve the analgesic effect. Methods Forty male Sprague Dawley rats were randomly divided into a normal group, a sham-model group, a model group and an EA group, 10 rats in each one. The rats in the normal group received no treatment; the rats in sham-model group were treated with operation to exposure sciatic nerve for 2 to 3 min (no knot); the rats in the remaining groups were treated with model establishment of chronic constrictive injury (CCI). 7 days after model establishment, the rats in the EA group were treated with EA at "Zusanli" (ST 36) and "Yanglingquan" (GB 34), 30 min per time, once a day for consecutive 7 days. Only immobilization was used in the remaining groups the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) of affected side feet were respectively measured before model establishment and 3 days, 5 days, 7 days, 10 days, 12 days and 14 days after model establishment; 14 days after model establishment, rats were sacrificed; the immunohistochemical method was used to measure the expression of Iba1 and BDNF in the sample of L_4 to L_6 spinal cord; real-time fluorescent quantitative PCR was used to measure the expression BDNF mRNA. Results Compared with the sham-model group, the pain threshold was decreased significantly in the model group (P<0.05), leading to hyperpathia. After EA treatment, compared with the model group, the pain threshold was increased significantly in the EA group (P<0.05). 14 days after operation, the microglia cells in the L_4 to L_6 spinal cord, expression of BDNF and level of mRNA in the model group were significantly higher than those in the normal group and sham-model group (all P<0.01); those in the EA group were significantly lower than those in the model group (all P<0.01). Conclusion The analgesic effect on neuropathic pain is likely to be achieved by EA through inhibiting the activation of spinal microglia cells and down-regulating the expression of BDNF.
资助项目	国家自然科学基金面上项目:81574074;浙江省自然科学基金资助项目:LY16H270016;温州市科技局资助项目:Y20140221
ISSN	0255-2930
卷号	37 期号:4 页码:411-416.
DOI	10.13703/j.0255-2930.2017.04.016
页数	6
收录类别	PUBMED ; CNKI ; 万方 ; 北大核心 ; PKU ; CSCD ; ISTIC
学科领域	医药、卫生 ; 中国医学
URL	查看原文
CSCD记录号	CSCD:5955355
PubMed ID	29231595
CNKI分类号	R245
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/34104
专题	第二临床医学院，附属第二医院、育英儿童医院_康复医学_康复科
作者单位	Department of Rehabilitation, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China.
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院_康复医学_康复科
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院_康复医学_康复科
推荐引用方式 GB/T 7714	Sisi Li,Pengpeng Gu,Wenzhan Tu,et al. [Effects of electroacupuncture on activation of microglia cells in spinal cord in rats with neuropathic pain][J]. Zhongguo zhen jiu = Chinese acupuncture &moxibustion,2017,37(4):411-416..
APA	Sisi Li., Pengpeng Gu., Wenzhan Tu., Xia Jiang., Wenci Chen., ... & Songhe Jiang. (2017). [Effects of electroacupuncture on activation of microglia cells in spinal cord in rats with neuropathic pain]. Zhongguo zhen jiu = Chinese acupuncture &moxibustion, 37(4), 411-416..
MLA	Sisi Li,et al."[Effects of electroacupuncture on activation of microglia cells in spinal cord in rats with neuropathic pain]".Zhongguo zhen jiu = Chinese acupuncture &moxibustion 37.4(2017):411-416..

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