Tumor protein P63 Regulated 1 contributes to inflammation and cell proliferation of cystitis glandularis through regulating the NF-kB/cyclooxygenase-2/ prostaglandin E2 axis

doi:10.17305/bjbms.2021.6763

科研成果详情

题名	Tumor protein P63 Regulated 1 contributes to inflammation and cell proliferation of cystitis glandularis through regulating the NF-kB/cyclooxygenase-2/ prostaglandin E2 axis
作者	Hong, Tao 1; Piao, Songzhe 2; Sun, Liangxue 2; Tao, Yiran 2; Ke, Mang2
发表日期	2022
发表期刊	BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Tumor protein P63 regulated 1 inflammation proliferation cystitis glandularis NF-kB cyclooxygenase 2 prostaglandin E2
其他关键词	NF-KAPPA-B ; EXPRESSION ; PATHWAY ; COX-2
摘要	Cystitis glandularis is characterized by chronic inflammation and hyperproliferation of the bladder mucosa, and it contributes to the progression of bladder adenocarcinoma. Tumor Protein P63 Regulated 1 (TPRG1) is related to cellular inflammatory response, and dysregulation of TPRG1 in tumor tissues is associated with tumor recurrence. The effect of TPRG1 on cystitis glandularis was investigated in this study. Firstly, bladder specimens were isolated from patients with cystitis glandularis and Escherichia coli-induced cystitis rat. Expression of TPRG1 was found to be up-regulated in the bladder specimen. Moreover, adeno-associated virus (AAV)-mediated silencing of TPRG1 was injected into rat, and data from hematoxylin and eosin (H & E) staining showed that injection with AAV-shTPRG1 ameliorated E. coli-induced histological changes in bladder tissues of rats and suppressed the inflammatory response. Secondly, TPRG1 was also increased in primary cystitis glandularis cells. Knockdown of TPRG1 decreased cell proliferation and suppressed the migration of primary cystitis glandularis cells. Thirdly, cyclooxygenase-2 (COX-2) was up-regulated in the bladder specimens isolated from patients with cystitis glandularis and E. coli-induced cystitis rat. Injection with AAV-shTPRG1 reduced protein expression of COX-2, p65 and prostaglandin E2 (PGE2) in the bladder specimen. Finally, interference of COX-2 attenuated TPRG1 over-expression-induced increase in cell proliferation and migration in the primary cystitis glandularis cells. In conclusion, TPRG1 promoted inflammation and cell proliferation of cystitis glandularis through activation of NF-kB/ COX2/PGE2 axis.
资助项目	Health science and Technology Project of Zhejiang Province [2021KY392]; Medical Science and Technology Plan of Zhejiang Province [2020KY354]
出版者	ASSOC BASIC MEDICAL SCI FEDERATION BOSNIA & HERZEGOVINA SARAJEVO
出版地	CEKALUSA
ISSN	1512-8601
EISSN	1840-4812
卷号	22 期号:1 页码:100-109
DOI	10.17305/bjbms.2021.6763
页数	10
WOS类目	Medicine, Research & Experimental
WOS研究方向	Research & Experimental Medicine
WOS记录号	WOS:000759323200010
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	34998360
PMC记录号	PMC8860313
SCOPUSEID	2-s2.0-85125001715
通讯作者地址	[Ke, Mang]Department of Urology,Taizhou Hospital of Zhejiang Province,Wenzhou Medical University,No. 150 Ximen Street, Zhejiang,Linhai,China
Scopus学科分类	Medicine (all)
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/3236
专题	温州医科大学
通讯作者	Ke, Mang
作者单位	1.Department of Urology,Taizhou Hospital of Zhejiang Province,Shaoxing University,Zhejiang,Linhai,China; 2.Department of Urology,Taizhou Hospital of Zhejiang Province,Wenzhou Medical University,No. 150 Ximen Street, Zhejiang,Linhai,China
推荐引用方式 GB/T 7714	Hong, Tao,Piao, Songzhe,Sun, Liangxue,et al. Tumor protein P63 Regulated 1 contributes to inflammation and cell proliferation of cystitis glandularis through regulating the NF-kB/cyclooxygenase-2/ prostaglandin E2 axis[J]. BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES,2022,22(1):100-109.
APA	Hong, Tao, Piao, Songzhe, Sun, Liangxue, Tao, Yiran, & Ke, Mang. (2022). Tumor protein P63 Regulated 1 contributes to inflammation and cell proliferation of cystitis glandularis through regulating the NF-kB/cyclooxygenase-2/ prostaglandin E2 axis. BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES, 22(1), 100-109.
MLA	Hong, Tao,et al."Tumor protein P63 Regulated 1 contributes to inflammation and cell proliferation of cystitis glandularis through regulating the NF-kB/cyclooxygenase-2/ prostaglandin E2 axis".BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES 22.1(2022):100-109.