A rapid and sensitive UPLC-MS/MS method for the determination of flibanserin in rat plasma: application to a pharmacokinetic study

doi:10.1186/s13065-019-0620-9

科研成果详情

题名	A rapid and sensitive UPLC-MS/MS method for the determination of flibanserin in rat plasma: application to a pharmacokinetic study
作者	He, Long 1; You, Wenting 2; Wang, Sa 3; Jiang, Tian1; Chen, Caiming 2
发表日期	2019-08-27
发表期刊	BMC CHEMISTRY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Flibanserin Rat plasma UHPLC-MS MS Pharmacokinetics
其他关键词	SEXUAL DESIRE DISORDER ; LIQUID-CHROMATOGRAPHY ; WOMEN ; PHARMACOLOGY ; EFFICACY ; SAMPLE
摘要	Background In this work, we aim to develop and validate a fast, simple, and sensitive method for the quantitative determination of flibanserin and the exploration of its pharmacokinetics. Methods Ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was the method of choice for this investigation and carbamazepine was selected as an internal standard (IS). The plasma samples were processed by one-step protein precipitation using acetonitrile. The highly selective chromatographic separation of flibanserin and carbamazepine (IS) was realised using an Agilent RRHD Eclipse Plus C18 (2.1 x 50 mm, 1.8 mu) column with a gradient mobile phase consisting of 0.1% formic acid in water and acetonitrile. The analytes were detected using positive-ion electrospray ionization mass spectrometry via multiple reaction monitoring (MRM). The target fragment ions were m/z 391.3 -> 161.3 for flibanserin and m/z 237.1 -> 194 for carbamazepine (IS). The method was validated by linear calibration plots over the range of 100-120,000 ng/mL for flibanserin (R-2 = 0.999) in rat plasma. Results The extraction recovery of flibanserin was in the range of 91.5-95.8%. The determined inter- and intra-day precision was below 12.0%, and the accuracy was from - 6.6 to 12.0%. No obvious matrix effect and astaticism was observed for flibanserin. The target analytes were long-lasting and stable in rat plasma for 12 h at room temperature, 48 h at 4 degrees C, 30 days at - 20 degrees C, as well as after three freeze-thaw cycles (from - 20 degrees C to room temperature). The proposed method has been fully validated and successfully applied to the pharmacokinetic study of flibanserin.
资助项目	Scientific Research fund of Taizhou Science and Technology Bureau [1401ky44]
出版者	BMC
出版地	LONDON
EISSN	2661-801X
卷号	13 期号:1
DOI	10.1186/s13065-019-0620-9
页数	8
WOS类目	Chemistry, Multidisciplinary
WOS研究方向	Chemistry
WOS记录号	WOS:000483369400001
收录类别	SSCI ; SCIE
PubMed ID	3146348
通讯作者地址	[Chen, Caiming]Wenzhou Med Univ, Dept Pharm, Affiliated Wenling Hosp, 190 Taiping South Rd, Wenling 317500, Zhejiang, Peoples R China.
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/2973
专题	其他_附属温岭医院（温岭市第一人民医院）药学院（分析测试中心）_药学系
通讯作者	Chen, Caiming
作者单位	1.Wenzhou Med Univ, Clin Lab, Affiliated Wenling Hosp, Wenling 317500, Peoples R China; 2.Wenzhou Med Univ, Dept Pharm, Affiliated Wenling Hosp, 190 Taiping South Rd, Wenling 317500, Zhejiang, Peoples R China; 3.Wenzhou Med Univ, Neurol Dept, Affiliated Wenling Hosp, Wenling 317500, Peoples R China
第一作者单位	其他_附属温岭医院（温岭市第一人民医院）
通讯作者单位	药学院（分析测试中心）_药学系
第一作者的第一单位	其他_附属温岭医院（温岭市第一人民医院）
推荐引用方式 GB/T 7714	He, Long,You, Wenting,Wang, Sa,et al. A rapid and sensitive UPLC-MS/MS method for the determination of flibanserin in rat plasma: application to a pharmacokinetic study[J]. BMC CHEMISTRY,2019,13(1).
APA	He, Long, You, Wenting, Wang, Sa, Jiang, Tian, & Chen, Caiming. (2019). A rapid and sensitive UPLC-MS/MS method for the determination of flibanserin in rat plasma: application to a pharmacokinetic study. BMC CHEMISTRY, 13(1).
MLA	He, Long,et al."A rapid and sensitive UPLC-MS/MS method for the determination of flibanserin in rat plasma: application to a pharmacokinetic study".BMC CHEMISTRY 13.1(2019).