Effects of Helicobacter pylori and Heat Shock Protein 70 on the Proliferation of Human Gastric Epithelial Cells

doi:10.1155/2014/794342

科研成果详情

题名	Effects of Helicobacter pylori and Heat Shock Protein 70 on the Proliferation of Human Gastric Epithelial Cells
作者	Tao, Liping1; Zou, Hai 2; Huang, Zhimin 1
发表日期	2014
发表期刊	Gastroenterology research and practice 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	IN-VITRO ; APOPTOSIS ; EXPRESSION ; PROTEIN ; HSP70 ; GROWTH ; INDUCTION ; MECHANISM ; CAGA
摘要	Infection of Helicobacter pylori (H. pylori) changed the proliferation of gastric epithelial cells and decreased the expression of heat shock protein 70 (HSP70). However, the effects of H. pylori on the proliferation of gastric epithelial cells and the roles of HSP70 during the progress need further investigation. Objective. To investigate the effects of Helicobacter pylori (H. pylori) and heat shock protein 70 (HSP70) on the proliferation of human gastric epithelial cells. Methods. H. pylori and a human gastric epithelial cell line (AGS) were cocultured. The proliferation of AGS cells was quantitated by an MTT assay, and the expression of HSP70 in AGS cells was detected by Western blotting. HSP70 expression in AGS cells was silenced by small interfering RNA (siRNA) to investigate the role of HSP70. The siRNA-treated AGS cells were cocultured with H. pylori and cell proliferation was measured by an MTT assay. Results. The proliferation of AGS cells was accelerated by coculturing with H. pylori for 4 and 8 h, but was suppressed at 24 and 48 h. HSP70 expression was decreased in AGS cells infected by H. pylori for 48 h. The proliferation in HSP70-silenced AGS cells was inhibited after coculturing with H. pylori for 24 and 48 h compared with the control group. Conclusions. Coculture of H. pylori altered the proliferation of gastric epithelial cells and decreased HSP70 expression. HSP70 knockdown supplemented the inhibitory effect of H. pylori on proliferation of epithelial cells. These results indicate that the effects of H. pylori on the proliferation of gastric epithelial cells at least partially depend on the decreased expression of HSP70 induced by the bacterium.
出版者	HINDAWI LTD
出版地	LONDON
ISSN	1687-6121
EISSN	1687-630X
卷号	2014 页码:794342
DOI	10.1155/2014/794342
页数	5
WOS类目	Gastroenterology & Hepatology
WOS研究方向	Gastroenterology & Hepatology
WOS记录号	WOS:000340403200001
收录类别	PUBMED ; SCOPUS ; SCIE
URL	查看原文
PubMed ID	25161665
PMC记录号	PMC4138794
SCOPUSEID	2-s2.0-84934997442
通讯作者地址	[Zou, Hai]Department of Hepatology, Sixth Affiliated Hospital of Wenzhou Medical University,Lishui, Zhejiang,323000,China
Scopus学科分类	Hepatology;Gastroenterology
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/23046
专题	附属第一医院其他_附属第六医院（丽水市人民医院）
通讯作者	Zou, Hai
作者单位	1.Department of Gastroenterology of the First Affiliated Hospital, Wenzhou Medical University,Wenzhou, Zhejiang,325000,China; 2.Department of Hepatology, Sixth Affiliated Hospital of Wenzhou Medical University,Lishui, Zhejiang,323000,China
第一作者单位	附属第一医院
通讯作者单位	温州医科大学
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Tao, Liping,Zou, Hai,Huang, Zhimin. Effects of Helicobacter pylori and Heat Shock Protein 70 on the Proliferation of Human Gastric Epithelial Cells[J]. Gastroenterology research and practice,2014,2014:794342.
APA	Tao, Liping, Zou, Hai, & Huang, Zhimin. (2014). Effects of Helicobacter pylori and Heat Shock Protein 70 on the Proliferation of Human Gastric Epithelial Cells. Gastroenterology research and practice, 2014, 794342.
MLA	Tao, Liping,et al."Effects of Helicobacter pylori and Heat Shock Protein 70 on the Proliferation of Human Gastric Epithelial Cells".Gastroenterology research and practice 2014(2014):794342.