Profiling and bioinformatics analyses of circular RNAs in myocardial ischemia/reperfusion injury model in mice

doi:10.4330/wjc.v17.i1.102147

科研成果详情

题名	Profiling and bioinformatics analyses of circular RNAs in myocardial ischemia/reperfusion injury model in mice
作者	Wang, Jiao-Ni 1; Zhou, Ying-Ying 2; Yu, Yong-Wei 3; Chen, Jun 4
发表日期	2025-01-26
发表期刊	WORLD JOURNAL OF CARDIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Rna-sequencing Circular RNA MicroRNA CeRNA Myocardial ischemia/reperfusion Bioinformatics analyses
其他关键词	ISCHEMIA-REPERFUSION INJURY ; ISCHAEMIA/REPERFUSION INJURY ; DYSFUNCTION ; MECHANISMS ; AUTOPHAGY ; PROTECTS ; DISEASE ; DEATH ; HEART
摘要	BACKGROUND Myocardial ischemia/reperfusion (I/R) injury, which is associated with high morbidity and mortality, is a main cause of unexpected myocardial injury after acute myocardial infarction. However, the underlying mechanism remains unclear. Circular RNAs (circRNAs), which are formed from protein-coding genes, can sequester microRNAs or proteins, modulate transcription and interfere with splicing. Authoritative studies suggest that circRNAs may play an important role in myocardial I/R injury. AIM To explore the role and mechanism of circRNAs in myocardial I/R injury. METHODS We constructed a myocardial I/R injury model using ligation of the left anterior descending coronary artery, and evaluated the success of the validated model using triphenyltetrazolium chloride and hematoxylin-eosin staining. Then, left ventricular samples from different groups were selected for mRNA-sequence, and differential gene screening was performed on the obtained results. The differentially obtained mRNAs were divided into up-regulated and down-regulated according to their expression levels, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis were performed, respectively. Then, the obtained circRNA and microRNA (miRNA) were paired for analysis, and the binding sites of miRNA and mRNA were virtual screened. Finally, the obtained circRNA, miRNA and mRNA were constructed by ceRNA mutual most useful network. RESULTS We used an RNA sequencing array to investigate the expression signatures of circRNAs in myocardial I/R injury using three samples from the I/R group and three samples from the sham group. A total of 142 upregulated and 121 downregulated circRNAs were found to be differentially expressed (fold change >= 2, P < 0.05). GO and KEGG functional analyses of these circRNAs were performed. GO analysis revealed that these circRNAs were involved mainly in cellular and intracellular processes. KEGG analysis demonstrated that 6 of the top 20 pathways were correlated with cell apoptosis. Furthermore, a circRNA-miRNA coexpression network and ceRNA network based on these genes were constructed, revealing that mmu-circ-0001452, mmu-circ-0001637, and mmu-circ-0000870 might be key regulators of myocardial I/R injury. CONCLUSION This research provides new insights into the mechanism of myocardial I/R, which mmu-circ-0001452, mmu-circ-0001637, and mmu-circ-0000870 are expected to be new therapeutic targets for myocardial I/R injury.
资助项目	Zhejiang Provincial Natural Science Foundation of China [LQ23H020004]; The Medical and Health Research Project of Zhejiang province [2024KY983]; Basic Medical Health Technology Project of Wenzhou Science and Technology Bureau [Y20210818, Y20210140]
出版者	BAISHIDENG PUBLISHING GROUP INC
ISSN	1949-8462
卷号	17 期号:1
DOI	10.4330/wjc.v17.i1.102147
页数	14
WOS类目	Cardiac & Cardiovascular Systems
WOS研究方向	Cardiovascular System & Cardiology
WOS记录号	WOS:001413493600008
收录类别	ESCI ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	3986622
SCOPUSEID	2-s2.0-85215662422
通讯作者地址	[Yu, Yong-Wei]Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Crit Care Med, 79 Qingchun Rd, Hangzhou 310000, Zhejiang, Peoples R China.
Scopus学科分类	Cardiology and Cardiovascular Medicine
SCOPUS_ID	SCOPUS_ID:85215662422
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/225574
专题	第二临床医学院，附属第二医院、育英儿童医院_内科学_内分泌附属第一医院
通讯作者	Yu, Yong-Wei; Chen, Jun
作者单位	1.Zhejiang Univ, Sir Run Run Shaw Hosp, Sch Med, Dept Diagnost Ultrasound & Echocardiog, Hangzhou 310002, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Affliated & Yuying Childrens Hosp 2, Dept Endocrinol, Wenzhou 325000, Zhejiang, Peoples R China; 3.Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Crit Care Med, 79 Qingchun Rd, Hangzhou 310000, Zhejiang, Peoples R China; 4.Wenzhou Med Univ, Cardiac Care Unit, Affiliated Hosp 1, Wenzhou 325000, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jiao-Ni,Zhou, Ying-Ying,Yu, Yong-Wei,et al. Profiling and bioinformatics analyses of circular RNAs in myocardial ischemia/reperfusion injury model in mice[J]. WORLD JOURNAL OF CARDIOLOGY,2025,17(1).
APA	Wang, Jiao-Ni, Zhou, Ying-Ying, Yu, Yong-Wei, & Chen, Jun. (2025). Profiling and bioinformatics analyses of circular RNAs in myocardial ischemia/reperfusion injury model in mice. WORLD JOURNAL OF CARDIOLOGY, 17(1).
MLA	Wang, Jiao-Ni,et al."Profiling and bioinformatics analyses of circular RNAs in myocardial ischemia/reperfusion injury model in mice".WORLD JOURNAL OF CARDIOLOGY 17.1(2025).